UniProtKB - Q5RDS0 (PLPL6_PONAB)
Protein
Patatin-like phospholipase domain-containing protein 6
Gene
PNPLA6
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Functioni
Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Catalyzes the hydrolysis of several naturally occurring membrane-associated lipids. Hydrolyzes lysophospholipids and monoacylglycerols, preferring the 1-acyl to the 2-acyl isomer. Does not catalyze hydrolysis of di- or triacylglycerols or fatty acid amides.By similarity
Catalytic activityi
- a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H+ + sn-glycerol 3-phosphocholineBy similarityEC:3.1.1.5By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H+ + hexadecanoate + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- a 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + H+ + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-sn-glycero-3-phosphate + H2O = H+ + hexadecanoate + sn-glycerol 3-phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Inhibited by a series a OPs such as mipafox (MPX), phenyl saligenin phosphate (PSP), phenyl dipentyl phosphinate (PDPP), diisopropyl fluorophosphate and paraoxon.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1004 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 1124 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 186 – 313 | cNMP 1Add BLAST | 128 | |
Nucleotide bindingi | 502 – 624 | cNMP 2Add BLAST | 123 | |
Nucleotide bindingi | 620 – 740 | cNMP 3Add BLAST | 121 |
GO - Molecular functioni
- lysophospholipase activity Source: UniProtKB
- phosphatidyl phospholipase B activity Source: UniProtKB-EC
GO - Biological processi
- lipid catabolic process Source: UniProtKB-KW
- phosphatidylcholine metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Patatin-like phospholipase domain-containing protein 6CuratedAlternative name(s): Neuropathy target esterase (EC:3.1.1.5By similarity) |
Gene namesi | Name:PNPLA6 Synonyms:NTE |
Organismi | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
Taxonomic identifieri | 9601 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass type III membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 50 | LumenalSequence analysisAdd BLAST | 50 | |
Transmembranei | 51 – 71 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 72 – 1365 | CytoplasmicSequence analysisAdd BLAST | 1294 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000292201 | 1 – 1365 | Patatin-like phospholipase domain-containing protein 6Add BLAST | 1365 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 11 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 345 | PhosphoserineBy similarity | 1 | |
Modified residuei | 352 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 353 | PhosphoserineBy similarity | 1 | |
Modified residuei | 363 | PhosphoserineBy similarity | 1 | |
Modified residuei | 411 | PhosphoserineBy similarity | 1 | |
Modified residuei | 455 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Glycosylated.By similarity
Keywords - PTMi
Glycoprotein, PhosphoproteinFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 971 – 1137 | PNPLAPROSITE-ProRule annotationAdd BLAST | 167 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 975 – 980 | GXGXXGPROSITE-ProRule annotation | 6 | |
Motifi | 1002 – 1006 | GXSXGPROSITE-ProRule annotation | 5 | |
Motifi | 1124 – 1126 | DGA/GPROSITE-ProRule annotation | 3 |
Sequence similaritiesi
Belongs to the NTE family.Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2968, Eukaryota |
InParanoidi | Q5RDS0 |
OrthoDBi | 253518at2759 |
Family and domain databases
CDDi | cd00038, CAP_ED, 3 hits |
Gene3Di | 2.60.120.10, 3 hits |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR018490, cNMP-bd-like IPR000595, cNMP-bd_dom IPR001423, LysoPLipase_patatin_CS IPR002641, PNPLA_dom IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 3 hits PF01734, Patatin, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 3 hits |
SUPFAMi | SSF51206, SSF51206, 3 hits SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50042, CNMP_BINDING_3, 3 hits PS51635, PNPLA, 1 hit PS01237, UPF0028, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5RDS0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGTSSHGLAT NSSGAKVAER DGFQDVPAPG EGAAGRICGA QPVPFVPQVL
60 70 80 90 100
GVMIGAGVAV VVTAVLILLV VRRLRVPKTP APDGPRYRFR KRDKVLFYGR
110 120 130 140 150
KIMRKVSQST SSLVDTSVSA TSRPRMKKKL KMLNIAKKIL RIQKETPTLQ
160 170 180 190 200
RKEPPPAVLE ADLTEGDLAN SHLPSEVLYM FKNVRVLGHF EKPLFLELCR
210 220 230 240 250
HMVFQRLGQG DYVFRPGQPD ASIYVVQDGL LELCLPGPDG KECVVKEVVP
260 270 280 290 300
GDSVNSLLSI LDVITGHQHP QRTVSARAAR DSTVLRLPVE AFSAVFAKYP
310 320 330 340 350
ESLVRVVQII MVRLQRVTFL ALHNYLGLTN ELFSHEIQPL RLFPSPGLPT
360 370 380 390 400
RTSPVRGSKR MVSTSATDEP RETPGRPPDP TGAPLPGPTG DPVKPTSLET
410 420 430 440 450
PSAPLLSRCV SMPGDISGLQ GGPRSDFDMA YERGRISVSL QEGASGGSLA
460 470 480 490 500
APARTPTQEP REQPAGACEY SYCEDESATG GCPFGPYQGR QTSSIFEAAK
510 520 530 540 550
RELAKLMRIE DPSLLNSRVL LHHAKAGTII ARQGDQDVSL HFVLWGCLHV
560 570 580 590 600
YQHMIDKAED VCLFVAQPGE LVGQLAVLTG EPLIFTLRAQ RDCTFLRISK
610 620 630 640 650
SDFYEIMRAQ PSVVLSAAHT VAARMSPFVR QMDFAIDWTA VEAGRALYRQ
660 670 680 690 700
GDRSDCTYIV LNGRLRSVIQ RGSGKKELVG EYGRGDLIGV VEALTRQPRA
710 720 730 740 750
TTVHAVRDTE LAKLPEGTLG HIKRRHPQVV TRLIHLLSQK ILGNLQQLQG
760 770 780 790 800
PFPGSGLGVP PHSELTNPAS NLATVAVLPV CAEVPMVAFT LELQHALQAI
810 820 830 840 850
GPTLLLNSDI IRARLGASAL DSIQEFRLSG WLAQQEDAHR IVLYQTDASL
860 870 880 890 900
TPWTVRCLRQ ADCILIVGLG DQEPTLGQLE QMLENTAVRA LKQLVLLHRE
910 920 930 940 950
EGAGPTRTVE WLNMRSWCSG HPHLRCPRRL FSRRSPAKLH ELYEKVFSRR
960 970 980 990 1000
ADRHSDFSRL ARVLTGNTIA LVLGGGGARG CSHIGVLKAL EEAGVPVDLV
1010 1020 1030 1040 1050
GGTSIGSFIG ALYAEERSAS RTKQRAREWA KSMTSVLEPV LDLTYPVTSM
1060 1070 1080 1090 1100
FTGSAFNRSI HRVFQDKQIE DLWLPYFNVT TDITASAMRV HKDGSLWRYV
1110 1120 1130 1140 1150
RASMTLSGYL PPLCDPKDGH LLMDGGYINN LPADIARSMG AKTVIAIDVG
1160 1170 1180 1190 1200
SQDETDLSTY GDSLSGWWLL WKRLNPWADK VKVPDMAEIQ SRLAYVSCVR
1210 1220 1230 1240 1250
QLEVVKSSSY CEYLRPPIDC FKTMDFGKFD QVYDVGYQYG KAVFGGWSRG
1260 1270 1280 1290 1300
NVIEKMLTDR RSTDLNESRR ADVLAYPSSG FTDLAEIVSR IEPPTSYVSD
1310 1320 1330 1340 1350
GCADGEESDC LTEYEEDAGP DCSRDEGGSP EGASPSTASE MEEEKSILRQ
1360
RRCLPQEPPG SATDA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR857832 mRNA Translation: CAH90087.1 |
RefSeqi | NP_001124999.1, NM_001131527.1 |
Genome annotation databases
GeneIDi | 100453292 |
KEGGi | pon:100453292 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR857832 mRNA Translation: CAH90087.1 |
RefSeqi | NP_001124999.1, NM_001131527.1 |
3D structure databases
SMRi | Q5RDS0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000010625 |
Genome annotation databases
GeneIDi | 100453292 |
KEGGi | pon:100453292 |
Organism-specific databases
CTDi | 10908 |
Phylogenomic databases
eggNOGi | KOG2968, Eukaryota |
InParanoidi | Q5RDS0 |
OrthoDBi | 253518at2759 |
Family and domain databases
CDDi | cd00038, CAP_ED, 3 hits |
Gene3Di | 2.60.120.10, 3 hits |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR018490, cNMP-bd-like IPR000595, cNMP-bd_dom IPR001423, LysoPLipase_patatin_CS IPR002641, PNPLA_dom IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 3 hits PF01734, Patatin, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 3 hits |
SUPFAMi | SSF51206, SSF51206, 3 hits SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50042, CNMP_BINDING_3, 3 hits PS51635, PNPLA, 1 hit PS01237, UPF0028, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLPL6_PONAB | |
Accessioni | Q5RDS0Primary (citable) accession number: Q5RDS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2007 |
Last sequence update: | December 21, 2004 | |
Last modified: | December 2, 2020 | |
This is version 76 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families