UniProtKB - Q5RD56 (CFLAR_PONAB)
Protein
CASP8 and FADD-like apoptosis regulator
Gene
CFLAR
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Functioni
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity (By similarity).By similarity
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: InterPro
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- regulation of apoptotic process Source: InterPro
Keywordsi
Biological process | Apoptosis |
Protein family/group databases
MEROPSi | C14.971 |
Names & Taxonomyi
Protein namesi | Recommended name: CASP8 and FADD-like apoptosis regulatorCleaved into the following 2 chains: |
Gene namesi | Name:CFLAR |
Organismi | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
Taxonomic identifieri | 9601 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000045838 | 1 – 376 | CASP8 and FADD-like apoptosis regulator subunit p43Add BLAST | 376 | |
ChainiPRO_0000045839 | 377 – 480 | CASP8 and FADD-like apoptosis regulator subunit p12By similarityAdd BLAST | 104 |
Post-translational modificationi
Proteolytically processed by CASP8 generating subunit p43 and p12.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 369 – 370 | Cleavage; by CASP8By similarity | 2 | |
Sitei | 376 – 377 | Cleavage; by CASP8By similarity | 2 |
Interactioni
Subunit structurei
TNFRSF6 stimulation triggers recruitment to the death-inducing signaling complex (DISC) formed by TNFRSF6, FADD and CASP8 (By similarity). A proteolytic fragment (p43) stays associated with the DISC (By similarity).
Interacts with RIPK1 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9601.ENSPPYP00000014600 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 73 | DED 1PROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 92 – 170 | DED 2PROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 435 | Not proteolytically processed and involved in apoptosis inhibitionBy similarityAdd BLAST | 435 | |
Regioni | 1 – 305 | Interaction with CASP8 propeptideBy similarityAdd BLAST | 305 | |
Regioni | 1 – 227 | Interaction with FADDBy similarityAdd BLAST | 227 | |
Regioni | 1 – 195 | Interaction with CASP8By similarityAdd BLAST | 195 | |
Regioni | 192 – 480 | Interaction with TRAF1 and TRAF2By similarityAdd BLAST | 289 | |
Regioni | 192 – 435 | Interaction with CASP3By similarityAdd BLAST | 244 | |
Regioni | 217 – 480 | Interaction with CASP8 subunits p18 and p10By similarityAdd BLAST | 264 | |
Regioni | 263 – 358 | CaspaseAdd BLAST | 96 | |
Regioni | 370 – 480 | Interaction with CASP8By similarityAdd BLAST | 111 |
Domaini
The caspase domain lacks the active site residues involved in catalysis.
Sequence similaritiesi
Belongs to the peptidase C14A family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3573, Eukaryota |
InParanoidi | Q5RD56 |
OrthoDBi | 939331at2759 |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR029030, Caspase-like_dom_sf IPR011029, DEATH-like_dom_sf IPR001875, DED_dom IPR002398, Pept_C14 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit |
Pfami | View protein in Pfam PF01335, DED, 1 hit |
SMARTi | View protein in SMART SM00115, CASc, 1 hit SM00031, DED, 2 hits |
SUPFAMi | SSF47986, SSF47986, 2 hits SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS50208, CASPASE_P20, 1 hit PS50168, DED, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5RD56-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAEVIHQVE EALDTDEKEM LLFSCRDVAI DVVPPNVRDL LDILRERGKL
60 70 80 90 100
SVGDLAELLY RVRRFDLLKR ILKMDRKAVE THLLRNPHLV SDYRVLMAEI
110 120 130 140 150
GEDLDKSDVS SLIFLMKDYM GRGKISKEKS FLDLVVELEK LNLVAPDQLD
160 170 180 190 200
LLEKCLKNIH RIDLKTKIQK YKQSVQGAGT SYRNVLQAAI QKSFKDPSNN
210 220 230 240 250
FRLHNGRSKE QRLKEQLGTQ QEPVKKSIQE SEAFLPQSVP EERYKMKSKP
260 270 280 290 300
LGICLIIDCI GNETELLRDT FTSLGYEVQK FLHLSMHGIS QILGQFACMP
310 320 330 340 350
EHRDYDSFVC VLVSRGGSQS VYGVDQTHSG LPLHHIRRMF MGDSCPYLAG
360 370 380 390 400
KPKIFFIQNY VVSEGQLEDS SLLEVDGPAM KNVEFKAQKR GLCTVHREAD
410 420 430 440 450
FFWSLCTADV SLLEWSHSSP SLYLQCLSQK LRQERKRPLL DLHIELNGYM
460 470 480
YDWNSRVSAK EKYYVWLQHT LRKKLIPSYT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858062 mRNA Translation: CAH90301.1 |
RefSeqi | NP_001125140.1, NM_001131668.1 |
Genome annotation databases
GeneIDi | 100172025 |
KEGGi | pon:100172025 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858062 mRNA Translation: CAH90301.1 |
RefSeqi | NP_001125140.1, NM_001131668.1 |
3D structure databases
SMRi | Q5RD56 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000014600 |
Protein family/group databases
MEROPSi | C14.971 |
Genome annotation databases
GeneIDi | 100172025 |
KEGGi | pon:100172025 |
Organism-specific databases
CTDi | 8837 |
Phylogenomic databases
eggNOGi | KOG3573, Eukaryota |
InParanoidi | Q5RD56 |
OrthoDBi | 939331at2759 |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR029030, Caspase-like_dom_sf IPR011029, DEATH-like_dom_sf IPR001875, DED_dom IPR002398, Pept_C14 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit |
Pfami | View protein in Pfam PF01335, DED, 1 hit |
SMARTi | View protein in SMART SM00115, CASc, 1 hit SM00031, DED, 2 hits |
SUPFAMi | SSF47986, SSF47986, 2 hits SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS50208, CASPASE_P20, 1 hit PS50168, DED, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CFLAR_PONAB | |
Accessioni | Q5RD56Primary (citable) accession number: Q5RD56 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2006 |
Last sequence update: | December 21, 2004 | |
Last modified: | December 2, 2020 | |
This is version 72 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries