UniProtKB - Q5RCN6 (CP4V2_PONAB)
Cytochrome P450 4V2
CYP4V2
Functioni
A cytochrome P450 monooxygenase involved in fatty acid metabolism in the eye. Catalyzes the omega-hydroxylation of polyunsaturated fatty acids (PUFAs) docosahexaenoate (DHA) and its precursor eicosapentaenoate (EPA), and may contribute to the homeostasis of these retinal PUFAs. Omega hydroxylates saturated fatty acids such as laurate, myristate and palmitate, the catalytic efficiency decreasing in the following order: myristate > laurate > palmitate (C14>C12>C16). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
By similarityCatalytic activityi
- dodecanoate + O2 + reduced [NADPH—hemoprotein reductase] = 12-hydroxydodecanoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- O2 + reduced [NADPH—hemoprotein reductase] + tetradecanoate = 14-hydroxytetradecanoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- hexadecanoate + O2 + reduced [NADPH—hemoprotein reductase] = 16-hydroxyhexadecanoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.80By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 + reduced [NADPH—hemoprotein reductase] = 20-hydroxy-(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 + reduced [NADPH—hemoprotein reductase] = 22-hydroxy-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.79By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Activity regulationi
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 329 | Heme; covalent, via 1 linkBy similarity | 1 | |
Metal bindingi | 467 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- long-chain fatty acid omega-hydroxylase activity Source: UniProtKB-EC
GO - Biological processi
- fatty acid omega-oxidation Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid metabolism |
Ligand | Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 4V2Alternative name(s): Docosahexaenoic acid omega-hydroxylase CYP4V2By similarity (EC:1.14.14.79By similarity) Long-chain fatty acid omega-monooxygenaseBy similarity (EC:1.14.14.80By similarity) |
Gene namesi | Name:CYP4V2 |
Organismi | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
Taxonomic identifieri | 9601 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 13 – 33 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051860 | 1 – 525 | Cytochrome P450 4V2Add BLAST | 525 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0157, Eukaryota KOG3627, Eukaryota |
InParanoidi | Q5RCN6 |
OrthoDBi | 1247045at2759 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAGLWLGLVW QKLLLWGAAS AVSLAGASLV LSLLQRVASY ARKWQQMRPI
60 70 80 90 100
PTVARAYPLV GHALLMKRDG REFFQQIIEY TEEYRHMPLL KLWVGPVPMV
110 120 130 140 150
ALYNAENVEV ILTSSRQIDK SSMYKFLEPW LGLGLLTSTG NKWRSRRKML
160 170 180 190 200
TPTFHFTILE DFLDIMNEQA NILVKKLEKH VNQEAFNCFF YITLCALDII
210 220 230 240 250
CETAMGKNIG AQSNDDSEYV RAVYRMSQMI FQRIKMPWLW LDLWYLMFKE
260 270 280 290 300
GWEHEKGLKI LHTFTNNVIA ERANEMNADE DCRGVGRGSA PSKNKRRAFL
310 320 330 340 350
DLLLSVTDDE GNRLSHEDIR EEVDTFMFEG HDTTAAAINW SLYLLGCNPE
360 370 380 390 400
VQQKVDHELD DVFGKSDRPA TVEDLKKLRY LECVIKETLR LFPSVPLFAR
410 420 430 440 450
SVSEDCEVAG YRVLKGTEAV IIPYALHRDP RYFPNPEEFQ PERFFPENAQ
460 470 480 490 500
GRHPYAYVPF SAGPRNCIGQ KFAVMEEKTI LSCILRHFWI ESNQKREELG
510 520
LEGQLILRPS NGIWIKLKRR DADEP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858234 mRNA Translation: CAH90471.1 |
RefSeqi | NP_001125245.1, NM_001131773.1 |
Genome annotation databases
GeneIDi | 100172140 |
KEGGi | pon:100172140 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858234 mRNA Translation: CAH90471.1 |
RefSeqi | NP_001125245.1, NM_001131773.1 |
3D structure databases
SMRi | Q5RCN6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000017045 |
Genome annotation databases
GeneIDi | 100172140 |
KEGGi | pon:100172140 |
Organism-specific databases
CTDi | 285440 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota KOG3627, Eukaryota |
InParanoidi | Q5RCN6 |
OrthoDBi | 1247045at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP4V2_PONAB | |
Accessioni | Q5RCN6Primary (citable) accession number: Q5RCN6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2005 |
Last sequence update: | December 21, 2004 | |
Last modified: | February 23, 2022 | |
This is version 79 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families