UniProtKB - Q5RCF8 (DHRS4_PONAB)
Dehydrogenase/reductase SDR family member 4
DHRS4
Functioni
NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones. Reduces 3-ketosteroids and benzil into 3beta-hydroxysteroids and R-benzoin, respectively, in contrast to the stereoselectivity of non-primate DHRS4s which produce 3alpha-hydroxysteroids and S-benzoin. Diplays low activity toward all-trans-retinal and no activity toward 9-cis-retinal as compared to non-primate mammals. In the reverse reaction, catalyze the NAD-dependent oxidation of 3beta-hydroxysteroids and alcohol, but with much lower efficiency. Involved in the metabolism of 3beta-hydroxysteroids, isatin and xenobiotic carbonyl compounds.
By similarityMiscellaneous
Catalytic activityi
- EC:1.1.1.184By similarityThis reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 169 | SubstrateBy similarity | 1 | |
Sitei | 176 | Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoinBy similarity | 1 | |
Sitei | 179 | Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoinBy similarity | 1 | |
Active sitei | 182 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 186 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 60 | NADPBy similarityAdd BLAST | 25 |
GO - Molecular functioni
- 3-keto sterol reductase activity Source: Ensembl
- alcohol dehydrogenase [NAD(P)+] activity Source: Ensembl
- carbonyl reductase (NADPH) activity Source: UniProtKB
- identical protein binding Source: UniProtKB
- oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor Source: Ensembl
GO - Biological processi
- alcohol metabolic process Source: Ensembl
- cellular ketone metabolic process Source: UniProtKB
- positive regulation of reactive oxygen species metabolic process Source: Ensembl
- steroid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Names & Taxonomyi
Protein namesi | Recommended name: Dehydrogenase/reductase SDR family member 4By similarity (EC:1.1.1.184By similarity)Alternative name(s): NADPH-dependent carbonyl reductaseBy similarity Short name: CRBy similarity NADPH-dependent retinol dehydrogenase/reductase Short name: NDRD Peroxisomal short-chain alcohol dehydrogenase Short name: PSCD Short chain dehydrogenase/reductase family 25C member 2By similarity Short name: Protein SDR25C2By similarity |
Gene namesi | Name:DHRS4 |
Organismi | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
Taxonomic identifieri | 9601 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Proteomesi |
|
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Peroxisome
- peroxisomal membrane Source: Ensembl
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054650 | 1 – 278 | Dehydrogenase/reductase SDR family member 4Add BLAST | 278 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 92 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 92 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 105 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 216 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 216 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 220 | PhosphoserineBy similarity | 1 | |
Modified residuei | 227 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 234 | N6-succinyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinInteractioni
Subunit structurei
Homotetramer.
By similarityGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000006452 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 276 – 278 | Peroxisomal targeting signal | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0725, Eukaryota |
InParanoidi | Q5RCF8 |
OrthoDBi | 1194344at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR029511, DHRS4-like IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PANTHERi | PTHR43943:SF8, PTHR43943:SF8, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MHKAGQLGLC ARAWNSVRMA SSGMTRRDPL ANKVALVTAS TDGIGFAIAR
60 70 80 90 100
RLAQDGAHVV VSSRKQQNVD QAVATLQGEG LSVTGTVCHV GKAEDRERLV
110 120 130 140 150
ATAVKLHGGI DILVSNAAVN PFFGSLMDVT EEVWDKTLDI NVKAPALMTK
160 170 180 190 200
AVVPEMEKRG GGSVVIVSSI AAFSPSPGFS PYNVSKTALL GLTKTLAIEL
210 220 230 240 250
APRNIRVNCL APGLIKTSFS RMLWMDKEKE ESMKETLRIR RLGEPEDCAG
260 270
IVSFLCSEDA SYITGETVVV GGGTPSRL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH2NKT8 | H2NKT8_PONAB | Dehydrogenase/reductase SDR family ... | DHRS4 | 278 | Annotation score: | ||
A0A8I5T2D6 | A0A8I5T2D6_PONAB | Dehydrogenase/reductase SDR family ... | DHRS4 | 172 | Annotation score: | ||
A0A8I5UBR7 | A0A8I5UBR7_PONAB | Dehydrogenase/reductase SDR family ... | DHRS4 | 155 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858312 mRNA Translation: CAH90549.1 CR858929 mRNA Translation: CAH91127.1 |
RefSeqi | NP_001125292.1, NM_001131820.2 XP_009247234.1, XM_009248959.1 |
Genome annotation databases
Ensembli | ENSPPYT00000006707; ENSPPYP00000006452; ENSPPYG00000005675 |
GeneIDi | 100172190 |
KEGGi | pon:100172190 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858312 mRNA Translation: CAH90549.1 CR858929 mRNA Translation: CAH91127.1 |
RefSeqi | NP_001125292.1, NM_001131820.2 XP_009247234.1, XM_009248959.1 |
3D structure databases
AlphaFoldDBi | Q5RCF8 |
SMRi | Q5RCF8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000006452 |
Genome annotation databases
Ensembli | ENSPPYT00000006707; ENSPPYP00000006452; ENSPPYG00000005675 |
GeneIDi | 100172190 |
KEGGi | pon:100172190 |
Organism-specific databases
CTDi | 10901 |
Phylogenomic databases
eggNOGi | KOG0725, Eukaryota |
InParanoidi | Q5RCF8 |
OrthoDBi | 1194344at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR029511, DHRS4-like IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PANTHERi | PTHR43943:SF8, PTHR43943:SF8, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHRS4_PONAB | |
Accessioni | Q5RCF8Primary (citable) accession number: Q5RCF8 Secondary accession number(s): Q5RAT3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | October 5, 2010 | |
Last modified: | May 25, 2022 | |
This is version 83 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families