UniProtKB - Q5RC71 (HXK1_PONAB)
Hexokinase-1
HK1
Functioni
Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine (By similarity).
Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity).
Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (By similarity).
By similarityCatalytic activityi
- EC:2.7.1.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.1.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.1.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.1.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.1.1By similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
: hexose metabolism Pathwayi
This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.
Pathwayi: glycolysis
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.By similarity This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 30 | ATP 1By similarity | 1 | |
Binding sitei | 155 | Substrate 1By similarity | 1 | |
Binding sitei | 209 | Glucose-6-phosphate 1By similarity | 1 | |
Binding sitei | 232 | Glucose-6-phosphate 1By similarity | 1 | |
Binding sitei | 235 | Substrate 1By similarity | 1 | |
Binding sitei | 260 | Substrate 1By similarity | 1 | |
Binding sitei | 345 | ATP 1By similarity | 1 | |
Binding sitei | 449 | Glucose-6-phosphate 1By similarity | 1 | |
Binding sitei | 657 | Glucose-6-phosphate 2By similarity | 1 | |
Binding sitei | 680 | ATP 2By similarity | 1 | |
Binding sitei | 680 | Glucose-6-phosphate 2By similarity | 1 | |
Binding sitei | 708 | Substrate 2By similarity | 1 | |
Binding sitei | 742 | Substrate 2By similarity | 1 | |
Binding sitei | 897 | Glucose-6-phosphate 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 84 – 89 | ATP 1By similarity | 6 | |
Nucleotide bindingi | 425 – 426 | ATP 1By similarity | 2 | |
Nucleotide bindingi | 532 – 537 | ATP 2By similarity | 6 | |
Nucleotide bindingi | 747 – 748 | ATP 2By similarity | 2 | |
Nucleotide bindingi | 784 – 788 | ATP 2By similarity | 5 | |
Nucleotide bindingi | 863 – 867 | ATP 2By similarity | 5 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- fructokinase activity Source: UniProtKB
- glucokinase activity Source: UniProtKB
- glucosamine kinase activity Source: RHEA
- glucose binding Source: InterPro
- mannokinase activity Source: UniProtKB
GO - Biological processi
- cellular glucose homeostasis Source: InterPro
- fructose 6-phosphate metabolic process Source: UniProtKB
- glucose 6-phosphate metabolic process Source: UniProtKB
- glycolytic process Source: UniProtKB-UniPathway
- inflammatory response Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- mannose metabolic process Source: UniProtKB
Keywordsi
Molecular function | Allosteric enzyme, Kinase, Transferase |
Biological process | Glycolysis, Immunity, Inflammatory response, Innate immunity |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00180 UPA00242 |
Names & Taxonomyi
Protein namesi | Recommended name: Hexokinase-1Curated (EC:2.7.1.1By similarity)Alternative name(s): Hexokinase type IBy similarity Short name: HK IBy similarity |
Gene namesi | Name:HK1By similarity |
Organismi | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
Taxonomic identifieri | 9601 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane By similarity; Peripheral membrane protein By similarity
Cytoplasm and Cytosol
- cytosol By similarity
Note: The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane. Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol.By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000286047 | 1 – 917 | Hexokinase-1Add BLAST | 917 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 337 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinInteractioni
Subunit structurei
Monomer (By similarity).
Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity).
Interacts with VDAC1. The HK1-VDAC1 complex interacts with ATF2 (By similarity).
Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9601.ENSPPYP00000002765 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 16 – 458 | Hexokinase 1PROSITE-ProRule annotationAdd BLAST | 443 | |
Domaini | 464 – 906 | Hexokinase 2PROSITE-ProRule annotationAdd BLAST | 443 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 10 | Mitochondrial-binding peptide (MBP)By similarity | 10 | |
Regioni | 73 – 207 | Hexokinase small subdomain 1PROSITE-ProRule annotationAdd BLAST | 135 | |
Regioni | 84 – 91 | Glucose-6-phosphate 1 bindingBy similarity | 8 | |
Regioni | 172 – 173 | Substrate 1 bindingBy similarity | 2 | |
Regioni | 208 – 447 | Hexokinase large subdomain 1PROSITE-ProRule annotationAdd BLAST | 240 | |
Regioni | 208 – 209 | Substrate 1 bindingBy similarity | 2 | |
Regioni | 291 – 294 | Substrate 1 bindingBy similarity | 4 | |
Regioni | 413 – 415 | Glucose-6-phosphate 1 bindingBy similarity | 3 | |
Regioni | 521 – 655 | Hexokinase small subdomain 2PROSITE-ProRule annotationAdd BLAST | 135 | |
Regioni | 532 – 536 | Glucose-6-phosphate 2 bindingBy similarity | 5 | |
Regioni | 603 – 604 | Substrate 2 bindingBy similarity | 2 | |
Regioni | 620 – 621 | Substrate 2 bindingBy similarity | 2 | |
Regioni | 656 – 895 | Hexokinase large subdomain 2PROSITE-ProRule annotationAdd BLAST | 240 | |
Regioni | 656 – 657 | Substrate 2 bindingBy similarity | 2 | |
Regioni | 682 – 683 | Substrate 2 bindingBy similarity | 2 | |
Regioni | 861 – 863 | Glucose-6-phosphate 2 bindingBy similarity | 3 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1369, Eukaryota |
InParanoidi | Q5RC71 |
OrthoDBi | 1153545at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR001312, Hexokinase IPR019807, Hexokinase_BS IPR022673, Hexokinase_C IPR022672, Hexokinase_N |
PANTHERi | PTHR19443, PTHR19443, 2 hits |
Pfami | View protein in Pfam PF00349, Hexokinase_1, 2 hits PF03727, Hexokinase_2, 2 hits |
SUPFAMi | SSF53067, SSF53067, 4 hits |
PROSITEi | View protein in PROSITE PS00378, HEXOKINASE_1, 2 hits PS51748, HEXOKINASE_2, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG
60 70 80 90 100
LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE
110 120 130 140 150
KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKSPV
160 170 180 190 200
GFTFSFPCQQ SKIDEAVLIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD
210 220 230 240 250
ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG
260 270 280 290 300
DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM
310 320 330 340 350
YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH
360 370 380 390 400
NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN
410 420 430 440 450
KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG
460 470 480 490 500
KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR
510 520 530 540 550
KQTHNNAAVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK
560 570 580 590 600
RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF
610 620 630 640 650
TFSFPCKQTS LDAGILITWT KGFKATDCVG NDVATLLRDA IKRREEFDLD
660 670 680 690 700
VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ
710 720 730 740 750
GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL
760 770 780 790 800
GEIVRNILID FTKKGFLFRG QISEPLKTRG IFETKFLSQI ESDRLALLQV
810 820 830 840 850
RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG
860 870 880 890 900
LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG
910
AALITAVGVR LRTEASS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858409 mRNA Translation: CAH90636.1 |
RefSeqi | NP_001125344.1, NM_001131872.1 |
Genome annotation databases
GeneIDi | 100172246 |
KEGGi | pon:100172246 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858409 mRNA Translation: CAH90636.1 |
RefSeqi | NP_001125344.1, NM_001131872.1 |
3D structure databases
SMRi | Q5RC71 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000002765 |
Genome annotation databases
GeneIDi | 100172246 |
KEGGi | pon:100172246 |
Organism-specific databases
CTDi | 3098 |
Phylogenomic databases
eggNOGi | KOG1369, Eukaryota |
InParanoidi | Q5RC71 |
OrthoDBi | 1153545at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00180 UPA00242 |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR001312, Hexokinase IPR019807, Hexokinase_BS IPR022673, Hexokinase_C IPR022672, Hexokinase_N |
PANTHERi | PTHR19443, PTHR19443, 2 hits |
Pfami | View protein in Pfam PF00349, Hexokinase_1, 2 hits PF03727, Hexokinase_2, 2 hits |
SUPFAMi | SSF53067, SSF53067, 4 hits |
PROSITEi | View protein in PROSITE PS00378, HEXOKINASE_1, 2 hits PS51748, HEXOKINASE_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | HXK1_PONAB | |
Accessioni | Q5RC71Primary (citable) accession number: Q5RC71 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | December 21, 2004 | |
Last modified: | April 7, 2021 | |
This is version 77 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families