UniProtKB - Q5RAZ4 (DHX15_PONAB)
ATP-dependent RNA helicase DHX15
DHX15
Functioni
RNA helicase involved in mRNA processing and antiviral innate immunity. Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. Plays a key role in antiviral innate immunity by promoting both MAVS-dependent signaling and NLRP6 inflammasome. Acts as an RNA virus sensor: recognizes and binds viral double stranded RNA (dsRNA) and activates the MAVS-dependent signaling to produce interferon-beta and interferon lambda-3 (IFNL3). Involved in intestinal antiviral innate immunity together with NLRP6: recognizes and binds viral dsRNA and promotes activation of the NLRP6 inflammasome in intestinal epithelial cells to restrict infection by enteric viruses. The NLRP6 inflammasome acts by promoting maturation and secretion of IL18 in the extracellular milieu (By similarity).
Also involved in antibacterial innate immunity by promoting Wnt-induced antimicrobial protein expression in Paneth cells (By similarity).
By similarityCatalytic activityi
- EC:3.6.4.13By similarity
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 160 – 167 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- double-stranded RNA binding Source: Ensembl
- RNA helicase activity Source: UniProtKB-EC
GO - Biological processi
- mRNA processing Source: UniProtKB-KW
- RNA splicing Source: UniProtKB-KW
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Immunity, Innate immunity, mRNA processing, mRNA splicing |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase DHX15Curated (EC:3.6.4.13By similarity)Alternative name(s): DEAH box protein 15 |
Gene namesi | Name:DHX15By similarity |
Organismi | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
Taxonomic identifieri | 9601 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Proteomesi |
|
Subcellular locationi
Nucleus
- nuclear speck Source: Ensembl
- nucleolus Source: UniProtKB-SubCell
- U12-type spliceosomal complex Source: Ensembl
- U2-type post-mRNA release spliceosomal complex Source: Ensembl
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000260306 | 1 – 795 | ATP-dependent RNA helicase DHX15Add BLAST | 795 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | PhosphoserineBy similarity | 1 | |
Modified residuei | 488 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 786 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationInteractioni
Subunit structurei
Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Identified in the Intron Large spliceosome complex (IL, also named intron lariat spliceosome), a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors; the association may be transient. The IL complex exists in two distinct conformations, one with the DHX15 (ILS2) and one without (ILS1).
Interacts with TFIP11 (via G-patch domain); indicative for a recruitment to the IL complex.
Interacts with SSB/La.
Interacts with GPATCH2 (via G-patch domain); promoting the RNA helicase activity.
Interacts with NKRF (via G-patch domain); promoting the RNA helicase activity.
Interacts with NLRP6.
By similarityProtein-protein interaction databases
STRINGi | 9601.ENSPPYP00000016358 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 147 – 313 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 167 | |
Domaini | 338 – 518 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 181 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 108 | DisorderedSequence analysisAdd BLAST | 108 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 260 – 263 | DEAH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 63 | Basic and acidic residuesSequence analysisAdd BLAST | 63 | |
Compositional biasi | 81 – 108 | Polar residuesSequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0925, Eukaryota |
InParanoidi | Q5RAZ4 |
OrthoDBi | 354219at2759 |
Family and domain databases
CDDi | cd17973, DEXHc_DHX15, 1 hit |
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR044756, DHX15_DEXHc IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE
60 70 80 90 100
REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSAH SAHSTHSAHS
110 120 130 140 150
THAGHAGHTS LPQCINPFTN LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL
160 170 180 190 200
VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS LPGPKRGVAC TQPRRVAAMS
210 220 230 240 250
VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM LLREAMNDPL
260 270 280 290 300
LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF
310 320 330 340 350
QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG
360 370 380 390 400
DLLLFLTGQE EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI
410 420 430 440 450
FEPPPPKKQN GAIGRKVVVS TNIAETSLTI DGVVFVIDPG FAKQKVYNPR
460 470 480 490 500
IRVESLLVTA ISKASAQQRA GRAGRTRPGK CFRLYTEKAY KTEMQDNTYP
510 520 530 540 550
EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL ELLNYLAALN
560 570 580 590 600
DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF
610 620 630 640 650
VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN
660 670 680 690 700
YRSLMSADNV RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ
710 720 730 740 750
VAHLERTGHY LTVKDNQVVQ LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC
760 770 780 790
TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL DRIIAKLQSK EYSQY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 312 | I → S in CAH91066 (Ref. 1) Curated | 1 | |
Sequence conflicti | 793 | S → L in CAH91066 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858867 mRNA Translation: CAH91066.1 CR926098 mRNA Translation: CAI29724.1 |
RefSeqi | NP_001127370.1, NM_001133898.1 |
Genome annotation databases
GeneIDi | 100174435 |
KEGGi | pon:100174435 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858867 mRNA Translation: CAH91066.1 CR926098 mRNA Translation: CAI29724.1 |
RefSeqi | NP_001127370.1, NM_001133898.1 |
3D structure databases
SMRi | Q5RAZ4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000016358 |
Genome annotation databases
GeneIDi | 100174435 |
KEGGi | pon:100174435 |
Organism-specific databases
CTDi | 1665 |
Phylogenomic databases
eggNOGi | KOG0925, Eukaryota |
InParanoidi | Q5RAZ4 |
OrthoDBi | 354219at2759 |
Family and domain databases
CDDi | cd17973, DEXHc_DHX15, 1 hit |
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR044756, DHX15_DEXHc IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHX15_PONAB | |
Accessioni | Q5RAZ4Primary (citable) accession number: Q5RAZ4 Secondary accession number(s): Q5NVD9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | June 12, 2007 | |
Last modified: | February 23, 2022 | |
This is version 86 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families