UniProtKB - Q5RAS0 (ACADS_PONAB)
Protein
Short-chain specific acyl-CoA dehydrogenase, mitochondrial
Gene
ACADS
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Functioni
Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains.By similarity
Catalytic activityi
- a short-chain 2,3-saturated fatty acyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = a short-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]By similarityEC:1.3.8.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- butanoyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityEC:1.3.8.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- H+ + oxidized [electron-transfer flavoprotein] + pentanoyl-CoA = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- H+ + hexanoyl-CoA + oxidized [electron-transfer flavoprotein] = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
FADBy similarityNote: Binds 1 FAD per subunit.By similarity
: mitochondrial fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway mitochondrial fatty acid beta-oxidation, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway mitochondrial fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 161 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 297 | FADBy similarity | 1 | |
Binding sitei | 297 | FAD; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 308 | FADBy similarity | 1 | |
Active sitei | 392 | Proton acceptorBy similarity | 1 | |
Binding sitei | 393 | Substrate; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 152 – 161 | FADBy similarity | 10 | |
Nucleotide bindingi | 185 – 187 | FADBy similarity | 3 | |
Nucleotide bindingi | 365 – 369 | FADBy similarity | 5 | |
Nucleotide bindingi | 394 – 396 | FADBy similarity | 3 |
GO - Molecular functioni
- acyl-CoA dehydrogenase activity Source: UniProtKB
- butyryl-CoA dehydrogenase activity Source: UniProtKB
- flavin adenine dinucleotide binding Source: InterPro
GO - Biological processi
- fatty acid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
UniPathwayi | UPA00660 |
Names & Taxonomyi
Protein namesi | Recommended name: Short-chain specific acyl-CoA dehydrogenase, mitochondrial (EC:1.3.8.1By similarity)Short name: SCAD Alternative name(s): Butyryl-CoA dehydrogenase |
Gene namesi | Name:ACADS |
Organismi | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
Taxonomic identifieri | 9601 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion matrix By similarity
Mitochondrion
- mitochondrial matrix Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 24 | MitochondrionBy similarityAdd BLAST | 24 | |
ChainiPRO_0000290193 | 25 – 412 | Short-chain specific acyl-CoA dehydrogenase, mitochondrialAdd BLAST | 388 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 27 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 51 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 51 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 72 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 129 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 129 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 208 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 262 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 262 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 306 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 306 | N6-succinyllysine; alternateBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinInteractioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 9601.ENSPPYP00000005742 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 269 – 272 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the acyl-CoA dehydrogenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0139, Eukaryota |
InParanoidi | Q5RAS0 |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5RAS0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAALLARAS GPVRRALRPR AWRQLHTIYQ SVELPETHQM LLQTCRDFAE
60 70 80 90 100
KELFPIAAQV DKEHLFPAAQ VKKMGGLGLL AMDVPEELGG AGLDYLAYAI
110 120 130 140 150
AMEEISRGCA STGVIMSVNN SLYLGPILKF GSKEQKQKWV TPFTSGDKIG
160 170 180 190 200
CFALSEPGNG SDAGAASTTA RAEGDSWVLN GTKAWITNAW EASAAVVFAS
210 220 230 240 250
TDRALQNKGI SAFLVPMPTP GLTLGKKEDK LGIRGSSTAN LIFEDCRIPK
260 270 280 290 300
DSILGEPGMG FKIAMQTLDM GRIGIASQAL GIAQTALDCA VNYAENRMAF
310 320 330 340 350
GAPLTKLQVI QFKLADMALA LESARLLTWR AAMLKDNKKP FIKEAAMAKL
360 370 380 390 400
AASEAATAIS HQAIQILGGM GYVTEMPAER HYRDARITEI YEGTSEIQRL
410
VIAGHLLRSY RS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858942 mRNA Translation: CAH91140.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858942 mRNA Translation: CAH91140.1 |
3D structure databases
SMRi | Q5RAS0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000005742 |
Phylogenomic databases
eggNOGi | KOG0139, Eukaryota |
InParanoidi | Q5RAS0 |
Enzyme and pathway databases
UniPathwayi | UPA00660 |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACADS_PONAB | |
Accessioni | Q5RAS0Primary (citable) accession number: Q5RAS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 12, 2007 |
Last sequence update: | December 21, 2004 | |
Last modified: | October 7, 2020 | |
This is version 74 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families