UniProtKB - Q5RAM3 (OPA1_PONAB)
Protein
Dynamin-like 120 kDa protein, mitochondrial
Gene
OPA1
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Functioni
Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission. Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (By similarity). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (By similarity). Plays a role in mitochondrial genome maintenance (By similarity).By similarity
Inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion.By similarity
Dynamin-like 120 kDa protein, form S2: Produced by cleavage at position S2 by YME1L. Promotes mitochondrial fusion.By similarity
Catalytic activityi
- EC:3.6.5.5By similarity
Activity regulationi
Activated by guanine nucleotide exchange factor RCC1L.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 295 – 302 | GTPSequence analysis | 8 | |
Nucleotide bindingi | 398 – 402 | GTPSequence analysis | 5 | |
Nucleotide bindingi | 467 – 470 | GTPSequence analysis | 4 |
GO - Molecular functioni
- cardiolipin binding Source: UniProtKB
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB-KW
- phosphatidic acid binding Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- GTP metabolic process Source: UniProtKB
- membrane tubulation Source: UniProtKB
- mitochondrial fusion Source: UniProtKB
- mitochondrial genome maintenance Source: UniProtKB
- response to stimulus Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Apoptosis, Sensory transduction |
Ligand | GTP-binding, Lipid-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Dynamin-like 120 kDa protein, mitochondrial (EC:3.6.5.5)Alternative name(s): Optic atrophy protein 1 homolog Cleaved into the following chain: |
Gene namesi | Name:OPA1By similarity |
Organismi | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
Taxonomic identifieri | 9601 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Single-pass membrane protein Sequence analysis
- Mitochondrion intermembrane space By similarity
- Mitochondrion membrane By similarity
Note: Detected at contact sites between endoplasmic reticulum and mitochondrion membranes.By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
- mitochondrial intermembrane space Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 88 – 96 | Mitochondrial matrixSequence analysis | 9 | |
Transmembranei | 97 – 113 | HelicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 114 – 960 | Mitochondrial intermembraneSequence analysisAdd BLAST | 847 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 87 | MitochondrionBy similarityAdd BLAST | 87 | |
ChainiPRO_0000257992 | 88 – 960 | Dynamin-like 120 kDa protein, mitochondrialAdd BLAST | 873 | |
ChainiPRO_0000257993 | 195 – 960 | Dynamin-like 120 kDa protein, form S1By similarityAdd BLAST | 766 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 228 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. Cleaved by OMA1 at position S1 following stress conditions.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 194 – 195 | Cleavage at site S1By similarity | 2 |
Keywords - PTMi
AcetylationProteomic databases
PRIDEi | Q5RAM3 |
Interactioni
Subunit structurei
Oligomeric complex consisting of membrane-bound and soluble forms of OPA1.
Interacts with CHCHD3 and IMMT; these interactions occur preferentially with soluble OPA1 forms.
Interacts with RCC1L; this interaction is direct (By similarity). Binds PARL (By similarity).
Interacts with PRELID1 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9601.ENSPPYP00000016126 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 285 – 561 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 277 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 295 – 302 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 321 – 324 | G2 motifPROSITE-ProRule annotation | 4 | |
Regioni | 398 – 401 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 467 – 470 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 501 – 504 | G5 motifPROSITE-ProRule annotation | 4 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 210 – 254 | Sequence analysisAdd BLAST | 45 | |
Coiled coili | 895 – 960 | Sequence analysisAdd BLAST | 66 |
Sequence similaritiesi
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coil, Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0447, Eukaryota |
InParanoidi | Q5RAM3 |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
InterProi | View protein in InterPro IPR001401, Dynamin_GTPase IPR022812, Dynamin_SF IPR030381, G_DYNAMIN_dom IPR033047, Opa1 IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 1 hit PTHR11566:SF67, PTHR11566:SF67, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5RAM3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWRLRRAAVA CEVCQSLVKH SSGIKGSLPL QKLHLVSRSI YHSHYPTLKL
60 70 80 90 100
QRPQLRTSFQ QFSSLTNLPL RKLKFSPIKY GYQPRRNFWP ARLATRLLKL
110 120 130 140 150
RYLILGSAVG GGYTAKKTFD QWKDMIPDLS EYKWIVPDIV WEIDEYIDFE
160 170 180 190 200
KIRKALPNSE DLVKLAPDFD KIVESLSLLK DFFTSGSPGE TAFRATDHGS
210 220 230 240 250
ESDKHFRKVS DKEKIDQLQE ELLHTQLKYQ RILERLEKEN KELRKLVLQK
260 270 280 290 300
DDKGIHHRKL KKSLIDMYSE VLDVLSDYDA SYNTQDHLPR VVVVGDQSAG
310 320 330 340 350
KTSVLEMIAQ ARIFPRGSGE MMTRSPVKVT LSEGPHHVAL FKDSSREFDL
360 370 380 390 400
TKEEDLAALR HEIELRMRKN VKEGCTVSPE TISLNVKGPG LQRMVLVDLP
410 420 430 440 450
GVINTVTSGM APDTKETIFS ISKAYMQNPN AIILCIQDGS VDAERSIVTD
460 470 480 490 500
LVSQMDPHGR RTIFVLTKVD LAEKNVASPS RIQQIIEGKL FPMKALGYFA
510 520 530 540 550
VVTGKGNSSE SIEAIREYEE EFFQNSKLLK TSMLKAHQVT TRNLSLAVSD
560 570 580 590 600
CFWKMVRESV EQQADSFKAT RFNLETEWKN NYPRLRELDR NELFEKAKNE
610 620 630 640 650
ILDEVISLSQ ATPKHWEEIL QQSLWERVST HVIENIYLPA AQTMNSGTFN
660 670 680 690 700
TTVDIKLKQW TDKQLPNKAV EVAWETLQGE FSRFMTEPKG KEHDDIFDKL
710 720 730 740 750
KEAVKEESIK RHKWNDFAED SLRVIQHNAL EDRSISDKQQ WDAAIYFMEE
760 770 780 790 800
ALQARLKDTE NAIENMVGPD WKKRWLYWKN RTQEQCVHNE TKNELEKMLK
810 820 830 840 850
CNEEHPAYLA SDEITTVRKN LESRGVEVDP SLIKDTWHQV YRRHFLKTAL
860 870 880 890 900
NHCNLCRRGF YYYQRHFVDS ELECNDVVLF WRIQRMLAIT ANTLRQQLTN
910 920 930 940 950
TEVRRLEKNV KEVLEDFAED GEKKIKLLTG KRVQLAEDLK KVREIQEKLD
960
AFIEALHQEK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858992 mRNA Translation: CAH91187.1 |
RefSeqi | NP_001125695.1, NM_001132223.1 |
Genome annotation databases
GeneIDi | 100172619 |
KEGGi | pon:100172619 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR858992 mRNA Translation: CAH91187.1 |
RefSeqi | NP_001125695.1, NM_001132223.1 |
3D structure databases
SMRi | Q5RAM3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000016126 |
Proteomic databases
PRIDEi | Q5RAM3 |
Genome annotation databases
GeneIDi | 100172619 |
KEGGi | pon:100172619 |
Organism-specific databases
CTDi | 4976 |
Phylogenomic databases
eggNOGi | KOG0447, Eukaryota |
InParanoidi | Q5RAM3 |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
InterProi | View protein in InterPro IPR001401, Dynamin_GTPase IPR022812, Dynamin_SF IPR030381, G_DYNAMIN_dom IPR033047, Opa1 IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 1 hit PTHR11566:SF67, PTHR11566:SF67, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPA1_PONAB | |
Accessioni | Q5RAM3Primary (citable) accession number: Q5RAM3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | December 21, 2004 | |
Last modified: | December 2, 2020 | |
This is version 85 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families