UniProtKB - Q5R4T1 (UBL4A_PONAB)
Protein
Ubiquitin-like protein 4A
Gene
UBL4A
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Functioni
As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates in their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome. Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome.By similarity
GO - Biological processi
- tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
Keywordsi
Biological process | Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-like protein 4A |
Gene namesi | Name:UBL4A |
Organismi | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
Taxonomic identifieri | 9601 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Proteomesi |
|
Subcellular locationi
Cytosol
- BAT3 complex Source: UniProtKB
- cytosol Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000403737 | 1 – 157 | Ubiquitin-like protein 4AAdd BLAST | 157 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 48 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 90 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Polyubiquitinated. Ubiquitination by AMFR and deubiquitination by USP13 may regulate the interaction between the BAG6/BAT complex and SGTA and therefore may regulate client proteins fate.By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationInteractioni
Subunit structurei
Component of the BAG6/BAT3 complex, at least composed of BAG6, UBL4A and GET4/TRC35.
Interacts with BAG6; the interaction is direct and required for UBL4A protein stability.
Interacts with USP13; may be indirect via BAG6.
By similarityProtein-protein interaction databases
STRINGi | 9601.ENSPPYP00000023375 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 76 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 96 – 138 | Required and sufficient for interaction with BAG6By similarityAdd BLAST | 43 |
Phylogenomic databases
eggNOGi | KOG0001, Eukaryota |
InParanoidi | Q5R4T1 |
OrthoDBi | 1586605at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS IPR019956, Ubiquitin_dom IPR041421, Ubl4_C_TUGS |
Pfami | View protein in Pfam PF17840, Tugs, 1 hit PF00240, ubiquitin, 1 hit |
PRINTSi | PR00348, UBIQUITIN |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5R4T1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQLTVKALQG RECSLQVPED ELVSTLKQLV SEKLNVPVRQ QRLLFKGKAL
60 70 80 90 100
ADGKRLSDYS IGPNSKLNLV VKPLEKVLLE EGEAQRLADS PPPQVWQLIS
110 120 130 140 150
KVLARHFSAA DASRVLEQPQ RDYERSLSRL TLDDIERLAS RFLHPEVTET
MEKGFSK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR861163 mRNA Translation: CAH93235.1 |
RefSeqi | NP_001126901.1, NM_001133429.1 |
Genome annotation databases
GeneIDi | 100173917 |
KEGGi | pon:100173917 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR861163 mRNA Translation: CAH93235.1 |
RefSeqi | NP_001126901.1, NM_001133429.1 |
3D structure databases
BMRBi | Q5R4T1 |
SMRi | Q5R4T1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9601.ENSPPYP00000023375 |
Genome annotation databases
GeneIDi | 100173917 |
KEGGi | pon:100173917 |
Organism-specific databases
CTDi | 8266 |
Phylogenomic databases
eggNOGi | KOG0001, Eukaryota |
InParanoidi | Q5R4T1 |
OrthoDBi | 1586605at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS IPR019956, Ubiquitin_dom IPR041421, Ubl4_C_TUGS |
Pfami | View protein in Pfam PF17840, Tugs, 1 hit PF00240, ubiquitin, 1 hit |
PRINTSi | PR00348, UBIQUITIN |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBL4A_PONAB | |
Accessioni | Q5R4T1Primary (citable) accession number: Q5R4T1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2011 |
Last sequence update: | December 21, 2004 | |
Last modified: | December 2, 2020 | |
This is version 70 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |