UniProtKB - Q5QGZ9 (CL12A_HUMAN)
Protein
C-type lectin domain family 12 member A
Gene
CLEC12A
Organism
Homo sapiens (Human)
Status
Functioni
Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases.2 Publications
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
GO - Biological processi
- neutrophil degranulation Source: Reactome
Keywordsi
Molecular function | Receptor |
Ligand | Lectin |
Enzyme and pathway databases
PathwayCommonsi | Q5QGZ9 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: C-type lectin domain family 12 member AAlternative name(s): C-type lectin-like molecule 1 Short name: CLL-1 Dendritic cell-associated lectin 2 Short name: DCAL-2 Myeloid inhibitory C-type lectin-like receptor Short name: MICL CD_antigen: CD371 |
Gene namesi | Name:CLEC12A Synonyms:CLL1, DCAL2, MICL |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000172322.13 |
HGNCi | HGNC:31713, CLEC12A |
MIMi | 612088, gene |
neXtProti | NX_Q5QGZ9 |
Subcellular locationi
Plasma membrane
- Cell membrane 4 Publications; Single-pass type II membrane protein 4 Publications
Note: Ligand binding leads to internalization.
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
- specific granule membrane Source: Reactome
- tertiary granule membrane Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 43 | CytoplasmicSequence analysisAdd BLAST | 43 | |
Transmembranei | 44 – 64 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 65 – 265 | ExtracellularSequence analysisAdd BLAST | 201 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 160364 |
OpenTargetsi | ENSG00000172322 |
PharmGKBi | PA142672094 |
Miscellaneous databases
Pharosi | Q5QGZ9, Tbio |
Chemistry databases
GuidetoPHARMACOLOGYi | 3081 |
Polymorphism and mutation databases
BioMutai | CLEC12A |
DMDMi | 308153619 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000313578 | 1 – 265 | C-type lectin domain family 12 member AAdd BLAST | 265 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 88 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 98 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 161 ↔ 248 | PROSITE-ProRule annotation | ||
Glycosylationi | 165 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 227 ↔ 240 | PROSITE-ProRule annotation |
Post-translational modificationi
Highly N-glycosylated. Glycosylation varies between cell types.3 Publications
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q5QGZ9 |
MassIVEi | Q5QGZ9 |
PaxDbi | Q5QGZ9 |
PeptideAtlasi | Q5QGZ9 |
PRIDEi | Q5QGZ9 |
ProteomicsDBi | 63611 [Q5QGZ9-2] 63612 [Q5QGZ9-1] 63613 [Q5QGZ9-3] 63614 [Q5QGZ9-4] 63615 [Q5QGZ9-5] |
PTM databases
GlyGeni | Q5QGZ9, 3 sites |
iPTMneti | Q5QGZ9 |
PhosphoSitePlusi | Q5QGZ9 |
Expressioni
Tissue specificityi
Detected in normal myeloid cells and in acute myeloid leukemia cells. Detected in neutrophils, eosinophils, monocytes and dendritic cells. Detected in spleen macrophage-rich red pulp and in lymph node (at protein level). Detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes and macrophages.4 Publications
Inductioni
Down-regulated in activated leukocytes recruited to a site of inflammation.1 Publication
Gene expression databases
Bgeei | ENSG00000172322, Expressed in monocyte and 129 other tissues |
ExpressionAtlasi | Q5QGZ9, baseline and differential |
Genevisiblei | Q5QGZ9, HS |
Organism-specific databases
HPAi | ENSG00000172322, Group enriched (blood, bone marrow, lung, lymphoid tissue) |
Interactioni
Subunit structurei
Interacts with PTPN6 and PTPN11.
1 PublicationBinary interactionsi
Q5QGZ9
With | #Exp. | IntAct |
---|---|---|
CLEC1A [Q8NC01] | 3 | EBI-13335829,EBI-11996768 |
Protein-protein interaction databases
BioGRIDi | 127754, 30 interactors |
IntActi | Q5QGZ9, 24 interactors |
STRINGi | 9606.ENSP00000347916 |
Miscellaneous databases
RNActi | Q5QGZ9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 140 – 249 | C-type lectinPROSITE-ProRule annotationAdd BLAST | 110 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 5 – 10 | ITIM motif | 6 |
Domaini
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00940000162719 |
InParanoidi | Q5QGZ9 |
OMAi | WHEDSCY |
OrthoDBi | 1201127at2759 |
TreeFami | TF336674 |
Family and domain databases
CDDi | cd03593, CLECT_NK_receptors_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR033992, NKR-like_CTLD |
Pfami | View protein in Pfam PF00059, Lectin_C, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS50041, C_TYPE_LECTIN_2, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 2 (identifier: Q5QGZ9-2) [UniParc]FASTAAdd to basket
Also known as: Alpha
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSEEVTYADL QFQNSSEMEK IPEIGKFGEK APPAPSHVWR PAALFLTLLC
60 70 80 90 100
LLLLIGLGVL ASMFHVTLKI EMKKMNKLQN ISEELQRNIS LQLMSNMNIS
110 120 130 140 150
NKIRNLSTTL QTIATKLCRE LYSKEQEHKC KPCPRRWIWH KDSCYFLSDD
160 170 180 190 200
VQTWQESKMA CAAQNASLLK INNKNALEFI KSQSRSYDYW LGLSPEEDST
210 220 230 240 250
RGMRVDNIIN SSAWVIRNAP DLNNMYCGYI NRLYVQYYHC TYKKRMICEK
260
MANPVQLGST YFREA
Isoform 3 (identifier: Q5QGZ9-3) [UniParc]FASTAAdd to basket
Also known as: Gamma
The sequence of this isoform differs from the canonical sequence as follows:
64-81: FHVTLKIEMKKMNKLQNI → CMYCPCFGQEEVSRISNI
82-265: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Curated
Show »Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA8MVL9 | A8MVL9_HUMAN | C-type lectin domain family 12 memb... | CLEC12A | 165 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_037669 | 244 | K → Q6 PublicationsCorresponds to variant dbSNP:rs479499Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039854 | 1 | M → MWIDFFTYSSM in isoform 1. 1 Publication | 1 | |
Alternative sequenceiVSP_030030 | 31 – 64 | APPAP…LASMF → V in isoform 4. 1 PublicationAdd BLAST | 34 | |
Alternative sequenceiVSP_030031 | 64 – 81 | FHVTL…KLQNI → CMYCPCFGQEEVSRISNI in isoform 3. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_030032 | 82 – 265 | Missing in isoform 3. 1 PublicationAdd BLAST | 184 | |
Alternative sequenceiVSP_030033 | 214 – 265 | Missing in isoform 5. 1 PublicationAdd BLAST | 52 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY547296 mRNA Translation: AAT11783.1 AY498550 mRNA Translation: AAS00605.1 AY498551 mRNA Translation: AAS00606.1 AY498552 mRNA Translation: AAS00607.1 AY426759 mRNA Translation: AAR84594.1 AF247788 mRNA Translation: AAL95693.1 AK314001 mRNA Translation: BAG36713.1 AC091814 Genomic DNA No translation available. CH471094 Genomic DNA Translation: EAW96133.1 BC063424 mRNA Translation: AAH63424.1 BC126289 mRNA Translation: AAI26290.1 BC126291 mRNA Translation: AAI26292.1 |
CCDSi | CCDS55803.1 [Q5QGZ9-1] CCDS73442.1 [Q5QGZ9-5] CCDS8608.1 [Q5QGZ9-2] CCDS8609.1 [Q5QGZ9-4] |
RefSeqi | NP_001193939.1, NM_001207010.1 [Q5QGZ9-1] NP_001287659.1, NM_001300730.1 [Q5QGZ9-5] NP_612210.4, NM_138337.5 [Q5QGZ9-2] NP_963917.2, NM_201623.3 [Q5QGZ9-4] XP_005253381.1, XM_005253324.2 [Q5QGZ9-2] XP_011518872.1, XM_011520570.1 [Q5QGZ9-2] |
Genome annotation databases
Ensembli | ENST00000304361; ENSP00000302804; ENSG00000172322 [Q5QGZ9-2] ENST00000350667; ENSP00000345448; ENSG00000172322 [Q5QGZ9-4] ENST00000355690; ENSP00000347916; ENSG00000172322 [Q5QGZ9-1] ENST00000434319; ENSP00000405244; ENSG00000172322 [Q5QGZ9-5] |
GeneIDi | 160364 |
KEGGi | hsa:160364 |
UCSCi | uc001qwq.3, human [Q5QGZ9-2] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY547296 mRNA Translation: AAT11783.1 AY498550 mRNA Translation: AAS00605.1 AY498551 mRNA Translation: AAS00606.1 AY498552 mRNA Translation: AAS00607.1 AY426759 mRNA Translation: AAR84594.1 AF247788 mRNA Translation: AAL95693.1 AK314001 mRNA Translation: BAG36713.1 AC091814 Genomic DNA No translation available. CH471094 Genomic DNA Translation: EAW96133.1 BC063424 mRNA Translation: AAH63424.1 BC126289 mRNA Translation: AAI26290.1 BC126291 mRNA Translation: AAI26292.1 |
CCDSi | CCDS55803.1 [Q5QGZ9-1] CCDS73442.1 [Q5QGZ9-5] CCDS8608.1 [Q5QGZ9-2] CCDS8609.1 [Q5QGZ9-4] |
RefSeqi | NP_001193939.1, NM_001207010.1 [Q5QGZ9-1] NP_001287659.1, NM_001300730.1 [Q5QGZ9-5] NP_612210.4, NM_138337.5 [Q5QGZ9-2] NP_963917.2, NM_201623.3 [Q5QGZ9-4] XP_005253381.1, XM_005253324.2 [Q5QGZ9-2] XP_011518872.1, XM_011520570.1 [Q5QGZ9-2] |
3D structure databases
SMRi | Q5QGZ9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 127754, 30 interactors |
IntActi | Q5QGZ9, 24 interactors |
STRINGi | 9606.ENSP00000347916 |
Chemistry databases
GuidetoPHARMACOLOGYi | 3081 |
PTM databases
GlyGeni | Q5QGZ9, 3 sites |
iPTMneti | Q5QGZ9 |
PhosphoSitePlusi | Q5QGZ9 |
Polymorphism and mutation databases
BioMutai | CLEC12A |
DMDMi | 308153619 |
Proteomic databases
jPOSTi | Q5QGZ9 |
MassIVEi | Q5QGZ9 |
PaxDbi | Q5QGZ9 |
PeptideAtlasi | Q5QGZ9 |
PRIDEi | Q5QGZ9 |
ProteomicsDBi | 63611 [Q5QGZ9-2] 63612 [Q5QGZ9-1] 63613 [Q5QGZ9-3] 63614 [Q5QGZ9-4] 63615 [Q5QGZ9-5] |
Protocols and materials databases
ABCDi | Q5QGZ9, 13 sequenced antibodies |
Antibodypediai | 11635, 372 antibodies |
DNASUi | 160364 |
Genome annotation databases
Ensembli | ENST00000304361; ENSP00000302804; ENSG00000172322 [Q5QGZ9-2] ENST00000350667; ENSP00000345448; ENSG00000172322 [Q5QGZ9-4] ENST00000355690; ENSP00000347916; ENSG00000172322 [Q5QGZ9-1] ENST00000434319; ENSP00000405244; ENSG00000172322 [Q5QGZ9-5] |
GeneIDi | 160364 |
KEGGi | hsa:160364 |
UCSCi | uc001qwq.3, human [Q5QGZ9-2] |
Organism-specific databases
CTDi | 160364 |
DisGeNETi | 160364 |
EuPathDBi | HostDB:ENSG00000172322.13 |
GeneCardsi | CLEC12A |
HGNCi | HGNC:31713, CLEC12A |
HPAi | ENSG00000172322, Group enriched (blood, bone marrow, lung, lymphoid tissue) |
MIMi | 612088, gene |
neXtProti | NX_Q5QGZ9 |
OpenTargetsi | ENSG00000172322 |
PharmGKBi | PA142672094 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00940000162719 |
InParanoidi | Q5QGZ9 |
OMAi | WHEDSCY |
OrthoDBi | 1201127at2759 |
TreeFami | TF336674 |
Enzyme and pathway databases
PathwayCommonsi | Q5QGZ9 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 160364, 3 hits in 839 CRISPR screens |
ChiTaRSi | CLEC12A, human |
GeneWikii | CLEC12A |
GenomeRNAii | 160364 |
Pharosi | Q5QGZ9, Tbio |
PROi | PR:Q5QGZ9 |
RNActi | Q5QGZ9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000172322, Expressed in monocyte and 129 other tissues |
ExpressionAtlasi | Q5QGZ9, baseline and differential |
Genevisiblei | Q5QGZ9, HS |
Family and domain databases
CDDi | cd03593, CLECT_NK_receptors_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR033992, NKR-like_CTLD |
Pfami | View protein in Pfam PF00059, Lectin_C, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS50041, C_TYPE_LECTIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CL12A_HUMAN | |
Accessioni | Q5QGZ9Primary (citable) accession number: Q5QGZ9 Secondary accession number(s): B2RA16 Q8TDQ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | October 5, 2010 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries