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Protein

Pleckstrin homology domain-containing family M member 1

Gene

Plekhm1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proposed to act as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Seems to be involved in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (By similarity). Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:17404618). May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7. May have a role in sialyl-lex-mediated transduction of apoptotic signals (By similarity). Involved in bone resorption (By similarity).By similarity1 Publication

Miscellaneous

Sialyl-lex is a carcinoma associated antigen.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri989 – 1043Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST55

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family M member 1
Short name:
PH domain-containing family M member 1
Gene namesi
Name:Plekhm1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1308010 Plekhm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003093371 – 1059Pleckstrin homology domain-containing family M member 1Add BLAST1059

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218PhosphoserineBy similarity1
Modified residuei430PhosphoserineBy similarity1
Modified residuei433PhosphoserineCombined sources1
Modified residuei488PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5PQS0
PRIDEiQ5PQS0

PTM databases

iPTMnetiQ5PQS0
PhosphoSitePlusiQ5PQS0

Expressioni

Tissue specificityi

Expressed in testis, skeletal muscle, lung, liver, spleen, brain, heart, kidney and bone. Weakly expressed in monocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000028521
GenevisibleiQ5PQS0 RN

Interactioni

Subunit structurei

Interacts (via N- and C-terminus) with RAB7A (GTP-bound form). Interacts with VPS41, VPS11 and VPS39; indicative for an association with the HOPS complex; the interaction with VPS41 seems to require RAB7A. Interacts with GABARAP, GABARAPL, GABARAPL2, MAP1LC3A, MAP1LC3B and MAP1LC3C. Interacts with PAFAH1B1; detected in osteoblasts. Interacts (via N- and C-terminus) with NDEL1. Interacts (via C-terminus) with MAP3K7. Interacts (via N- and C-terminus) with FAM98A. Interacts (via C-terminus) with DEF8; this interaction is weak but increased in a RAB7A-dependent manner. May interact with sialyl-lex positive protein.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034752

Structurei

3D structure databases

ProteinModelPortaliQ5PQS0
SMRiQ5PQS0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 182RUNPROSITE-ProRule annotationAdd BLAST143
Domaini536 – 627PH 1PROSITE-ProRule annotationAdd BLAST92
Domaini686 – 780PH 2PROSITE-ProRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni657 – 1059Interaction with RAB7ABy similarityAdd BLAST403

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi634 – 640LIRBy similarity7

Domaini

The LIR (LC3-interacting region) motif mediates the interaction with ATG8 family proteins GABARAP, GABARAPL, GABARAPL2, and LC3A/B/C.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri989 – 1043Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST55

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410KDQT Eukaryota
ENOG4111EZ2 LUCA
GeneTreeiENSGT00550000074570
HOGENOMiHOG000247013
HOVERGENiHBG056799
InParanoidiQ5PQS0
OMAiPAFFKII
OrthoDBiEOG091G013Y
PhylomeDBiQ5PQS0
TreeFamiTF317067

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR004012 Run_dom
IPR037213 Run_dom_sf
IPR025258 Zf-RING_9
PfamiView protein in Pfam
PF02759 RUN, 1 hit
PF13901 zf-RING_9, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM01175 DUF4206, 1 hit
SM00233 PH, 2 hits
SM00593 RUN, 1 hit
SUPFAMiSSF140741 SSF140741, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 2 hits
PS50826 RUN, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q5PQS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSVENGLDP RAAIQVIKKK LVGSVKALQK QHVSLDTVVT SEDGDANTMC
60 70 80 90 100
SALEAVFIHG LHAKYIRAEA GGKRKKHTHQ KPLPQPVFWP LLKAVTHKHI
110 120 130 140 150
ISDLEHLVFI NTDVGRCRAW LRLALNDGLM ECYLKLLLQE PARLCEYYQP
160 170 180 190 200
TALLRDAEEA EFLLSFLQGL TSLSFELSYK SAILNEWTLT PLSLSGLCPL
210 220 230 240 250
SELDPLTVSG AELQRKESLD SISHSSGSED IEVQHSGHKI RRDRKLTASS
260 270 280 290 300
LSLDTASSSQ LSCSLNSDSC LLQENGPKSP DHSEEPMSYD SDLGTANADD
310 320 330 340 350
SDRSLQEVLS EFSKAQVNSA PSSGPSQESD TPMFQTPLSL HSLANSTHLL
360 370 380 390 400
FEGSEEPFPA HTSSGTSSGH KHQPQESPDM QPLGTAQAGP AGSTSDQQPS
410 420 430 440 450
SPVAGAADQG SEPWKALEYG RVGPKLVVSS PTSPKGKSWI SEDDFCRPPK
460 470 480 490 500
EHALKNTSDL CISPLQGTPE LRTALHGPFS QGPRKSCSLG ALDKACVSSL
510 520 530 540 550
DYRNAQTAPS PAVTQDHKNF CVVHRRQMGL SNPFRGLMKL GTVARRGAMG
560 570 580 590 600
IWKEFFCELS PLELRLYLSD EERTCVESCS LLRCEAVGPA HSDGRFELVF
610 620 630 640 650
SGKKLALRAS SQDEAEDWLD RVREALQKVR PQQEEEWVNI QYPDQAEDSP
660 670 680 690 700
EAPPDNLPPY SALLPEHAGA QGIQPNWTSA QVPEPDAIKE SLLYLYADRT
710 720 730 740 750
WIPYIFSLSL ESLKCFRVRN NEKMLSDSHG VETIRDILPD TSLGGPAFFK
760 770 780 790 800
IITAKAVLKL QAKNTEEAAH WRDLVRKVLA SYLESVQEAV TLAGSLDENC
810 820 830 840 850
QEVLKFATRE NGFLLQYLVA IPTEKGLDSQ GCFCAGCSRQ IGFSFVRPKL
860 870 880 890 900
CAFSGLYYCD FCHQDDASVI PARIIHNWDL TKRPVCRQAL KFLAQIRAQP
910 920 930 940 950
LINLQLVNAS LYEHVERMHL IGRSREQLKL LGDYLGLCRS GALKELSKRL
960 970 980 990 1000
SHRNYLLESP HKFSVADLQQ IAEGVYEGFL KALIEFASQH VYHCDLCTQR
1010 1020 1030 1040 1050
GFICQICHHQ DIIFPFEFDT TVRCAECRTV FHQSCQAVVR KGCPRCARRR

KYQEQNTVS
Length:1,059
Mass (Da):117,508
Last modified:January 4, 2005 - v1
Checksum:i3D41F53BF6382CAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087058 mRNA Translation: AAH87058.1
RefSeqiNP_001009677.1, NM_001009677.1
UniGeneiRn.101335

Genome annotation databases

EnsembliENSRNOT00000037147; ENSRNOP00000034752; ENSRNOG00000028521
GeneIDi303584
KEGGirno:303584
UCSCiRGD:1308010 rat

Similar proteinsi

Entry informationi

Entry nameiPKHM1_RAT
AccessioniPrimary (citable) accession number: Q5PQS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: January 4, 2005
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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