Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Secreted effector protein PipB2

Gene

pipB2

Organism
Salmonella paratyphi A (strain ATCC 9150 / SARB42)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Involved in the reorganization of late endosome/lysosome (LE/Lys) compartments in mammalian cells. Necessary and sufficient to link kinesin-1 onto the Salmonella-containing vacuole (SCV) membrane. Required for centrifugal extension of lysosomal glycoprotein-rich membrane tubules, known as Salmonella-induced filaments (Sifs), away from the SCV and toward the cell periphery. Required for virulence, but not for intracellular survival and replication in phagocytic cells (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processVirulence

Enzyme and pathway databases

BioCyciSENT295319:G1G44-2815-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted effector protein PipB2
Alternative name(s):
Type III effector PipB2
Gene namesi
Name:pipB2
Ordered Locus Names:SPA2636
OrganismiSalmonella paratyphi A (strain ATCC 9150 / SARB42)
Taxonomic identifieri295319 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000008185 Componenti: Chromosome

Subcellular locationi

  • Secreted By similarity
  • Host membrane By similarity
  • Note: Secreted via the type III secretion system 2 (SPI-2 TTSS), and delivered into the host cell.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782971 – 350Secreted effector protein PipB2Add BLAST350

Interactioni

Subunit structurei

Interacts with the host kinesin light chain (KLC), a subunit of the kinesin-1 motor complex.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5PEX4
SMRiQ5PEX4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 201Pentapeptide repeat 1Add BLAST40
Domaini202 – 241Pentapeptide repeat 2Add BLAST40
Domaini247 – 286Pentapeptide repeat 3Add BLAST40
Domaini287 – 326Pentapeptide repeat 4Add BLAST40

Domaini

Contains various tandem pentapeptide repeats in the C-terminal region. The pentapeptide motif is required to efficiently recruit kinesin-1. No position is completely conserved in these repeats, whose consensus sequence is A-[DN]-[FLM]-X-X. The C-terminal 38 amino acid residues, specifically, the C-terminal motif LFNEF, are required for peripheral localization of PipB2 and redistribution of lysosomal-associated membrane protein (LAMP). The N-terminal 225 amino acid residues are sufficient for type III translocation and association with Sifs and SCVs, but not accumulation in peripheral vesicles (By similarity).By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000028269
KOiK15352
OMAiFNVFYSE

Family and domain databases

InterProiView protein in InterPro
IPR001646 5peptide_repeat
PfamiView protein in Pfam
PF00805 Pentapeptide, 4 hits

Sequencei

Sequence statusi: Complete.

Q5PEX4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQRSLDSLAG MATSAFGAGT SAAMRQATSP KTILEYIINF FTCGGIRRRN
60 70 80 90 100
ETQYQELIET MAETLKSTMP DRGAPLPENI ILDDMDGCRV EFNLPGENNE
110 120 130 140 150
AGQVIVRVSK GDHSETREIP LVSFEKICRA LLFRCEFSLP QDSVILTAQG
160 170 180 190 200
GMNLKGAVLT GANLTAENLC DADLSGANLE GAVLFMADCE GANFKGANLS
210 220 230 240 250
GTSLGDSNFK NACLEDGIMC GATLDHANLT GANLQHASLL GCSMIECNCS
260 270 280 290 300
GANMDHTNLS GATLIRADMS GATLQGATIM AAIMEDAVLT RANLRKASFI
310 320 330 340 350
STNLDGADLA EANLNNTCFK DCTLTHLRTE DATMSTSTQT LFNEFYSENI
Length:350
Mass (Da):37,308
Last modified:January 4, 2005 - v1
Checksum:i9C9E6CE13007CC79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000026 Genomic DNA Translation: AAV78500.1
RefSeqiWP_011233153.1, NC_006511.1

Genome annotation databases

EnsemblBacteriaiAAV78500; AAV78500; SPA2636
KEGGispt:SPA2636

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000026 Genomic DNA Translation: AAV78500.1
RefSeqiWP_011233153.1, NC_006511.1

3D structure databases

ProteinModelPortaliQ5PEX4
SMRiQ5PEX4
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV78500; AAV78500; SPA2636
KEGGispt:SPA2636

Phylogenomic databases

HOGENOMiHOG000028269
KOiK15352
OMAiFNVFYSE

Enzyme and pathway databases

BioCyciSENT295319:G1G44-2815-MONOMER

Family and domain databases

InterProiView protein in InterPro
IPR001646 5peptide_repeat
PfamiView protein in Pfam
PF00805 Pentapeptide, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPIPB2_SALPA
AccessioniPrimary (citable) accession number: Q5PEX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: January 4, 2005
Last modified: February 28, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again