UniProtKB - Q5PEN7 (AAS_SALPA)
Protein
Bifunctional protein Aas
Gene
aas
Organism
Salmonella paratyphi A (strain ATCC 9150 / SARB42)
Status
Functioni
Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1.UniRule annotation
Catalytic activityi
- a 2-acyl-sn-glycero-3-phosphoethanolamine + a fatty acyl-[ACP] = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + holo-[ACP]UniRule annotationEC:2.3.1.40UniRule annotation
- a long-chain fatty acid + ATP + holo-[ACP] = a long-chain fatty acyl-[ACP] + AMP + diphosphateUniRule annotationEC:6.2.1.20UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 36 | UniRule annotation | 1 |
GO - Molecular functioni
- acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-KW
- long-chain fatty acid [acyl-carrier-protein] ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- fatty acid metabolic process Source: InterPro
- phospholipid biosynthetic process Source: InterPro
Keywordsi
Molecular function | Acyltransferase, Ligase, Multifunctional enzyme, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | SENT295319:G1G44-3068-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional protein AasUniRule annotationIncluding the following 2 domains: Alternative name(s): 2-acyl-GPE acyltransferaseUniRule annotation Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferaseUniRule annotation Alternative name(s): Acyl-ACP synthetaseUniRule annotation Long-chain-fatty-acid--[acyl-carrier-protein] ligaseUniRule annotation |
Gene namesi | Name:aasUniRule annotation Ordered Locus Names:SPA2875 |
Organismi | Salmonella paratyphi A (strain ATCC 9150 / SARB42) |
Taxonomic identifieri | 295319 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 258 – 277 | HelicalUniRule annotationAdd BLAST | 20 | |
Transmembranei | 409 – 433 | HelicalUniRule annotationAdd BLAST | 25 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193051 | 1 – 719 | Bifunctional protein AasAdd BLAST | 719 |
Proteomic databases
PRIDEi | Q5PEN7 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 138 | AcyltransferaseAdd BLAST | 124 | |
Regioni | 233 – 646 | AMP-bindingAdd BLAST | 414 |
Sequence similaritiesi
In the N-terminal section; belongs to the 2-acyl-GPE acetyltransferase family.UniRule annotation
In the C-terminal section; belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
HOGENOMi | HOG000004907 |
KOi | K05939 |
OMAi | ANWVYLE |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
HAMAPi | MF_01162 Aas, 1 hit |
InterProi | View protein in InterPro IPR023775 Aas IPR020845 AMP-binding_CS IPR000873 AMP-dep_Synth/Lig IPR042099 AMP-dep_Synthh-like_sf IPR002123 Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553 Acyltransferase, 1 hit PF00501 AMP-binding, 1 hit |
SMARTi | View protein in SMART SM00563 PlsC, 1 hit |
PROSITEi | View protein in PROSITE PS00455 AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5PEN7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLFGFFRNLF RVLYRVRVTG DVRALQGNRV LITPNHVSFI DGMLLALFLP
60 70 80 90 100
VRPVFAVYTS ISQQWYMRWL TPLIDFVPLD PTKPMSIKHL MRLVEQGRPV
110 120 130 140 150
VIFPEGRISV TGSLMKIYDG AGFVAAKSGA TVIPLRIDGA ELTPFSRLKG
160 170 180 190 200
LVKRRLFPRI QLHILPPTQI PMPEAPRARD RRKIAGEMLH QIMMEARMAV
210 220 230 240 250
RPRETLYESL LAAQYRYGAG KNCIEDINFT PDTYRKLLTK TLFVGRILEK
260 270 280 290 300
YSVEGEKIGL MLPNAAISAA VIFGAVSRRR IPAMMNYTAG VKGLTSAIAA
310 320 330 340 350
AEIKTIFTSR QFLDKGKLWH LPEQLTQVRW VYLEDLKADV TLADKLWIFA
360 370 380 390 400
HLLAPRLAQV KQQPEDAAII LFTSGSEGHP KGVVHSHKSI LANVEQIKTI
410 420 430 440 450
ADFTANDRFM SALPLFHSFG LTVGLFTPLL TGAEVFLYPS PLHYRIVPEL
460 470 480 490 500
VYDRNCTVLF GTSTFLGNYA RFANPYDFYR LRYVVAGAEK LQESTKQLWQ
510 520 530 540 550
DKFGLRILEG YGVTECAPVV SINVPMAAKP GTVGRILPGM DARLLAVPGI
560 570 580 590 600
ENGGRLQLKG PNIMNGYLRV EKPGVLEVPS AENARGETER GWYDTGDIVR
610 620 630 640 650
FDENGFVQIQ GRAKRFAKIA GEMVSLEMVE QLALGVSADK MHATAIKSDA
660 670 680 690 700
SKGEALVLFT TDSELTREKL QHYAREHGIP ELAVPRDIRY LKQLPLLGSG
710
KPDFVTLKSW VDAPEQHHE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000026 Genomic DNA Translation: AAV78720.1 |
RefSeqi | WP_000896086.1, NC_006511.1 |
Genome annotation databases
EnsemblBacteriai | AAV78720; AAV78720; SPA2875 |
KEGGi | spt:SPA2875 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000026 Genomic DNA Translation: AAV78720.1 |
RefSeqi | WP_000896086.1, NC_006511.1 |
3D structure databases
SMRi | Q5PEN7 |
ModBasei | Search... |
Proteomic databases
PRIDEi | Q5PEN7 |
Genome annotation databases
EnsemblBacteriai | AAV78720; AAV78720; SPA2875 |
KEGGi | spt:SPA2875 |
Phylogenomic databases
HOGENOMi | HOG000004907 |
KOi | K05939 |
OMAi | ANWVYLE |
Enzyme and pathway databases
BioCyci | SENT295319:G1G44-3068-MONOMER |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
HAMAPi | MF_01162 Aas, 1 hit |
InterProi | View protein in InterPro IPR023775 Aas IPR020845 AMP-binding_CS IPR000873 AMP-dep_Synth/Lig IPR042099 AMP-dep_Synthh-like_sf IPR002123 Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553 Acyltransferase, 1 hit PF00501 AMP-binding, 1 hit |
SMARTi | View protein in SMART SM00563 PlsC, 1 hit |
PROSITEi | View protein in PROSITE PS00455 AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AAS_SALPA | |
Accessioni | Q5PEN7Primary (citable) accession number: Q5PEN7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | January 4, 2005 | |
Last modified: | May 8, 2019 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteomeDocuments
- SIMILARITY comments
Index of protein domains and families