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Entry version 77 (31 Jul 2019)
Sequence version 1 (01 Feb 2005)
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Protein

2-hydroxyisoflavanone dehydratase

Gene

HIDH

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Dehydratase that mediates the biosynthesis of isoflavonoids. Can use both 4'-hydroxylated and 4'-methoxylated 2-hydroxyisoflavanones as substrates. Has also a slight carboxylesterase activity toward p-nitrophenyl butyrate.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 1.6 sec(-1) with 2,7-dihydroxy-4'-methoxyisoflavanone, 5.3 sec(-1) with 2,7,4'-trihydroxyisoflavanone and 18.1 sec(-1) with 2,5,7,4'-tetrahydroxyisoflavanone as substrates, respectively (at pH 7.5 and 30 degrees Celsius).
  1. KM=29 µM for 2,7-dihydroxy-4'-methoxyisoflavanone (at pH 7.5 and 30 degrees Celsius)1 Publication
  2. KM=114 µM for 2,7,4'-trihydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius)1 Publication
  3. KM=170 µM for 2,5,7,4'-tetrahydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius)1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: flavonoid biosynthesis

    This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei1641 Publication1
    Active sitei2631 Publication1
    Active sitei2951 Publication1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionHydrolase, Lyase
    Biological processFlavonoid biosynthesis

    Enzyme and pathway databases

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    4.2.1.105 2483

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    Q5NUF3

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00154

    Protein family/group databases

    ESTHER database of the Alpha/Beta-hydrolase fold superfamily of proteins

    More...
    ESTHERi
    soybn-q5nuf3 Plant_carboxylesterase

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    2-hydroxyisoflavanone dehydratase (EC:3.1.1.1, EC:4.2.1.105)
    Alternative name(s):
    Carboxylesterase HIDH
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:HIDH
    Ordered Locus Names:Glyma01g45020
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiGlycine max (Soybean) (Glycine hispida)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3847 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideae50 kb inversion cladeNPAAA cladeindigoferoid/millettioid cladePhaseoleaeGlycineSoja
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000008827 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi78G → A: Reduction of both dehydratase and carboxylesterase activities. 1 Publication1
    Mutagenesisi79G → A: Reduction of both dehydratase and carboxylesterase activities. 1 Publication1
    Mutagenesisi164T → A or S: Reduction of both dehydratase and carboxylesterase activities. 1 Publication1
    Mutagenesisi263D → N: Complete loss of both dehydratase and carboxylesterase activities. 1 Publication1
    Mutagenesisi295H → A: Complete loss of both dehydratase and carboxylesterase activities. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004241011 – 3192-hydroxyisoflavanone dehydrataseAdd BLAST319

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    Gene expression databases

    Genevisible search portal to normalized and curated expression data from Genevestigator

    More...
    Genevisiblei
    Q5NUF3 GM

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    3847.GLYMA01G45020.1

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q5NUF3

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi77 – 79Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole1 Publication3

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the 'GDXG' lipolytic enzyme family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG1515 Eukaryota
    COG0657 LUCA

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    Q5NUF3

    KEGG Orthology (KO)

    More...
    KOi
    K13258

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    IDNPWIN

    Database of Orthologous Groups

    More...
    OrthoDBi
    1263520at2759

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.40.50.1820, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR029058 AB_hydrolase
    IPR013094 AB_hydrolase_3
    IPR002168 Lipase_GDXG_HIS_AS

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF07859 Abhydrolase_3, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF53474 SSF53474, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS01173 LIPASE_GDXG_HIS, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    Q5NUF3-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MAKEIVKELL PLIRVYKDGS VERLLSSENV AASPEDPQTG VSSKDIVIAD
    60 70 80 90 100
    NPYVSARIFL PKSHHTNNKL PIFLYFHGGA FCVESAFSFF VHRYLNILAS
    110 120 130 140 150
    EANIIAISVD FRLLPHHPIP AAYEDGWTTL KWIASHANNT NTTNPEPWLL
    160 170 180 190 200
    NHADFTKVYV GGETSGANIA HNLLLRAGNE SLPGDLKILG GLLCCPFFWG
    210 220 230 240 250
    SKPIGSEAVE GHEQSLAMKV WNFACPDAPG GIDNPWINPC VPGAPSLATL
    260 270 280 290 300
    ACSKLLVTIT GKDEFRDRDI LYHHTVEQSG WQGELQLFDA GDEEHAFQLF
    310
    KPETHLAKAM IKRLASFLV
    Length:319
    Mass (Da):35,138
    Last modified:February 1, 2005 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE8333CF425FBA4A3
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AB154415 mRNA Translation: BAD80840.1
    BT097440 mRNA Translation: ACU22699.1
    CM000834 Genomic DNA No translation available.

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_001237228.1, NM_001250299.2

    Genome annotation databases

    Ensembl plant genome annotation project

    More...
    EnsemblPlantsi
    KRH77876; KRH77876; GLYMA_01G239600

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    547489

    Gramene; a comparative resource for plants

    More...
    Gramenei
    KRH77876; KRH77876; GLYMA_01G239600

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    gmx:547489

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB154415 mRNA Translation: BAD80840.1
    BT097440 mRNA Translation: ACU22699.1
    CM000834 Genomic DNA No translation available.
    RefSeqiNP_001237228.1, NM_001250299.2

    3D structure databases

    SMRiQ5NUF3
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi3847.GLYMA01G45020.1

    Protein family/group databases

    ESTHERisoybn-q5nuf3 Plant_carboxylesterase

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiKRH77876; KRH77876; GLYMA_01G239600
    GeneIDi547489
    GrameneiKRH77876; KRH77876; GLYMA_01G239600
    KEGGigmx:547489

    Phylogenomic databases

    eggNOGiKOG1515 Eukaryota
    COG0657 LUCA
    InParanoidiQ5NUF3
    KOiK13258
    OMAiIDNPWIN
    OrthoDBi1263520at2759

    Enzyme and pathway databases

    UniPathwayiUPA00154
    BRENDAi4.2.1.105 2483
    SABIO-RKiQ5NUF3

    Gene expression databases

    GenevisibleiQ5NUF3 GM

    Family and domain databases

    Gene3Di3.40.50.1820, 1 hit
    InterProiView protein in InterPro
    IPR029058 AB_hydrolase
    IPR013094 AB_hydrolase_3
    IPR002168 Lipase_GDXG_HIS_AS
    PfamiView protein in Pfam
    PF07859 Abhydrolase_3, 1 hit
    SUPFAMiSSF53474 SSF53474, 1 hit
    PROSITEiView protein in PROSITE
    PS01173 LIPASE_GDXG_HIS, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHIDH_SOYBN
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q5NUF3
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: February 1, 2005
    Last modified: July 31, 2019
    This is version 77 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
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