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Protein

Biotin synthase

Gene

bioB

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB), Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi74Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi78Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi81Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi118Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi150Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi210Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi282Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciZMOB264203:G1FZK-78-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:ZMO0094
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
Proteomesi
  • UP000001173 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003817291 – 335Biotin synthaseAdd BLAST335

Proteomic databases

PRIDEiQ5NRD6

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5NRD6
SMRiQ5NRD6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000239957
KOiK01012
OMAiADRFCMG

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Q5NRD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSVGTQSHS GRDQAEQNPS VENVRYDWQL DEALAIYNLP LLELIGRANS
60 70 80 90 100
VHRRYHDAGH LQKSSLLSIK TGGCPEDCGY CSQSAHHDVE LTREKLMNPT
110 120 130 140 150
AVIGLAAKAK KAGAERFCMG AAWRKVRDGK EFDAVLEMIR GVRALDMEAC
160 170 180 190 200
VTLGMVNEDQ ARRLAEAGLT AYNHNLDTGP AYYPQIVSTH SYQDRLETLA
210 220 230 240 250
KIRDAGIALC TGGIIGLGES PRDRVEMLVV LAGMNPHPES VPVNMLVPIE
260 270 280 290 300
GTPLADADPV DPLEIVRMIA TARLMMPQSM VRLSAGRSTL SRETQILCLV
310 320 330
AGANSIFYGN VLLTTPNADM AADDALLEAL GVTAE
Length:335
Mass (Da):36,263
Last modified:March 23, 2010 - v2
Checksum:iB02CEABF178FF96B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008692 Genomic DNA Translation: AAV88718.2
RefSeqiWP_011240066.1, NZ_CP023715.1

Genome annotation databases

EnsemblBacteriaiAAV88718; AAV88718; ZMO0094
KEGGizmo:ZMO0094

Similar proteinsi

Entry informationi

Entry nameiBIOB_ZYMMO
AccessioniPrimary (citable) accession number: Q5NRD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 23, 2010
Last modified: July 18, 2018
This is version 91 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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