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UniProtKB - Q5NDL0 (EOGT_RAT)
Protein
EGF domain-specific O-linked N-acetylglucosamine transferase
Gene
Eogt
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.
By similarityCatalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-(N-acetyl-β-D-glucosaminyl)-L-seryl-[protein] + H+ + UDPBy similarityEC:2.4.1.255By similarity
- L-threonyl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-(N-acetyl-β-D-glucosaminyl)-L-threonyl-[protein] + H+ + UDPBy similarityEC:2.4.1.255By similarity
GO - Molecular functioni
- glycosyltransferase activity Source: GO_Central
- protein N-acetylglucosaminyltransferase activity Source: UniProtKB
- protein O-GlcNAc transferase activity Source: UniProtKB-EC
GO - Biological processi
- protein O-GlcNAcylation via threonine Source: GO_Central
- protein O-linked glycosylation Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
Names & Taxonomyi
Protein namesi | Recommended name: EGF domain-specific O-linked N-acetylglucosamine transferase (EC:2.4.1.255By similarity)Alternative name(s): Extracellular O-linked N-acetylglucosamine transferase |
Gene namesi | Name:Eogt Synonyms:Aer61 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1359357, Eogt |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen PROSITE-ProRule annotation
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: GO_Central
Keywords - Cellular componenti
Endoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000301974 | 20 – 527 | EGF domain-specific O-linked N-acetylglucosamine transferaseAdd BLAST | 508 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 354 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
jPOSTi | Q5NDL0 |
PaxDbi | Q5NDL0 |
PRIDEi | Q5NDL0 |
PTM databases
GlyGeni | Q5NDL0, 1 site |
PhosphoSitePlusi | Q5NDL0 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000024533, Expressed in lung and 20 other tissues |
ExpressionAtlasi | Q5NDL0, baseline and differential |
Genevisiblei | Q5NDL0, RN |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 295 – 297 | Required for optimal activityBy similarity | 3 | |
Motifi | 524 – 527 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Sequence similaritiesi
Belongs to the glycosyltransferase 61 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4698, Eukaryota |
GeneTreei | ENSGT00940000156493 |
HOGENOMi | CLU_039300_0_0_1 |
InParanoidi | Q5NDL0 |
PhylomeDBi | Q5NDL0 |
TreeFami | TF313716 |
Family and domain databases
InterProi | View protein in InterPro IPR007657, Glycosyltransferase_61 |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, Glyco_transf_61, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q5NDL0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLMLLVFGVL LHEVPLSGQD EAHPEADRVP GEALYDYSSL RLPEEHIPFF
60 70 80 90 100
LHSNRHVASV CREDSHCPYK KHLESLNSCW GYEKSCTPES RFGSPICSYV
110 120 130 140 150
DLGWTDTLES AQDMFWKQAD FGYARERLEE IRMFCRPESA SDSSLLCSRY
160 170 180 190 200
LQYCRATGLY LDLRNIKRNH DRFKEDFLQG GDIGGYCKLD RHALVSEGQR
210 220 230 240 250
KSPLQSWFAE LQGYTQLNFR PIEDAKCDIV VEKPTYFMKL DAGINMYHHF
260 270 280 290 300
CDFLNLYLTQ HINNSFSTDV YIVMWDTSSY GYGDLFSDTW KAFTDYDVIH
310 320 330 340 350
LKTYDSKKVC FKEAVFSLLP RMRYGLFYNT PLISGCQNTG LFRAFSQHVL
360 370 380 390 400
HRLNISQEGP KDGKLRVTIL ARSTEYRKIL NQNELVNALK TVSTFEVRVV
410 420 430 440 450
DYKYRELGFL DQLRITHNTD IFIGMHGAGL THLLFLPDWA AVFELYNCED
460 470 480 490 500
ERCYLDLARL RGIYYITWQK PSKVFPQDKG HHPTLGEHPK FTNYSFDVEE
510 520
FMYLVLQAAE HVLQHPQWPL KKNHDEL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K4R9 | A0A0G2K4R9_RAT | EGF domain-specific O-linked N-acet... | Eogt | 565 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ868236 mRNA Translation: CAI30571.1 |
RefSeqi | NP_001009502.1, NM_001009502.1 XP_008761369.1, XM_008763147.2 XP_017448256.1, XM_017592767.1 |
Genome annotation databases
Ensembli | ENSRNOT00000039353; ENSRNOP00000034561; ENSRNOG00000024533 |
GeneIDi | 494219 |
KEGGi | rno:494219 |
UCSCi | RGD:1359357, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ868236 mRNA Translation: CAI30571.1 |
RefSeqi | NP_001009502.1, NM_001009502.1 XP_008761369.1, XM_008763147.2 XP_017448256.1, XM_017592767.1 |
3D structure databases
SMRi | Q5NDL0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000034561 |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
PTM databases
GlyGeni | Q5NDL0, 1 site |
PhosphoSitePlusi | Q5NDL0 |
Proteomic databases
jPOSTi | Q5NDL0 |
PaxDbi | Q5NDL0 |
PRIDEi | Q5NDL0 |
Genome annotation databases
Ensembli | ENSRNOT00000039353; ENSRNOP00000034561; ENSRNOG00000024533 |
GeneIDi | 494219 |
KEGGi | rno:494219 |
UCSCi | RGD:1359357, rat |
Organism-specific databases
CTDi | 285203 |
RGDi | 1359357, Eogt |
Phylogenomic databases
eggNOGi | KOG4698, Eukaryota |
GeneTreei | ENSGT00940000156493 |
HOGENOMi | CLU_039300_0_0_1 |
InParanoidi | Q5NDL0 |
PhylomeDBi | Q5NDL0 |
TreeFami | TF313716 |
Miscellaneous databases
PROi | PR:Q5NDL0 |
Gene expression databases
Bgeei | ENSRNOG00000024533, Expressed in lung and 20 other tissues |
ExpressionAtlasi | Q5NDL0, baseline and differential |
Genevisiblei | Q5NDL0, RN |
Family and domain databases
InterProi | View protein in InterPro IPR007657, Glycosyltransferase_61 |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, Glyco_transf_61, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EOGT_RAT | |
Accessioni | Q5NDL0Primary (citable) accession number: Q5NDL0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | February 1, 2005 | |
Last modified: | February 23, 2022 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families