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Protein

E3 ubiquitin-protein ligase RNF43

Gene

Rnf43

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway (PubMed:22895187). Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification (By similarity).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri272 – 313RING-type; atypicalPROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processUbl conjugation pathway, Wnt signaling pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-4641263 Regulation of FZD by ubiquitination
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF43 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 43
RING-type E3 ubiquitin transferase RNF43Curated
Gene namesi
Name:Rnf43
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2442609 Rnf43

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 197ExtracellularSequence analysisAdd BLAST174
Transmembranei198 – 218HelicalSequence analysisAdd BLAST21
Topological domaini219 – 784CytoplasmicSequence analysisAdd BLAST566

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Conditional knockout mice lacking both Rnf43 and Znrf3 in intestine show a marked expansion of the proliferative compartment, resembling the effects of acute deletion of Apc.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000027824024 – 784E3 ubiquitin-protein ligase RNF43Add BLAST761

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi62N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi91 ↔ 119By similarity
Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Autoubiquitinated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5NCP0
PRIDEiQ5NCP0

PTM databases

PhosphoSitePlusiQ5NCP0

Expressioni

Tissue specificityi

Expressed in crypt base columnar cells of small intestinal epithelium. Crypt base columnar cells are small cycling cells residing between the terminally differentiated Paneth cells at crypt bottoms. Colocalizes with Lgr5-positive stem cells.1 Publication

Developmental stagei

At 14.5 dpc, in the developing limb, expressed only in the ectoderm and, in developing lungs, detected in the epithelium.1 Publication

Gene expression databases

BgeeiENSMUSG00000034177 Expressed in 127 organ(s), highest expression level in cochlea
CleanExiMM_RNF43
ExpressionAtlasiQ5NCP0 baseline and differential
GenevisibleiQ5NCP0 MM

Interactioni

Subunit structurei

Interacts with AKAP8L, NONO and SFPQ. Interacts with FZD5 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-59915N
IntActiQ5NCP0, 1 interactor
STRINGi10090.ENSMUSP00000090476

Structurei

3D structure databases

ProteinModelPortaliQ5NCP0
SMRiQ5NCP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi443 – 503Ser-richAdd BLAST61
Compositional biasi547 – 557His-richAdd BLAST11
Compositional biasi569 – 741Pro-richAdd BLAST173

Sequence similaritiesi

Belongs to the ZNRF3 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri272 – 313RING-type; atypicalPROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG0800 Eukaryota
ENOG41121N2 LUCA
GeneTreeiENSGT00760000119057
HOGENOMiHOG000246992
HOVERGENiHBG093916
InParanoidiQ5NCP0
KOiK15694
OrthoDBiEOG091G01HM
PhylomeDBiQ5NCP0
TreeFamiTF317074

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF13639 zf-RING_2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5NCP0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGGHQLQLA VLWPWLLMAT LHAGFGHTGR VLAAAVESER SAEQKAVIRV
60 70 80 90 100
IPLKMDPTGK LNLTLEGVFA GVAEVTPAEG KLMQSHPLYL CNASDDDNLE
110 120 130 140 150
PGFISIVKLE SPRRAPRPCL SLASKARMAG ERGANAVLFD ITEDRSAAEQ
160 170 180 190 200
LQQPLGLTKP VVLIWGSDAA KLMEFVYKNR KAYVWIELKE PPAGANYDVW
210 220 230 240 250
ILLTVVGTVF VIILASVLRI RCRPHHSRPD PLQQRTARAI SQLATRRYQA
260 270 280 290 300
GCRRARAEWP DSGSSCSSTP VCAICLEEFS EGQELRVISC LHEFHRTCVD
310 320 330 340 350
PWLYQHRTCP LCMFNIVEGD SFSQAPAASP SYQEPGRRLH LIRQHPGHAH
360 370 380 390 400
YHLPSAYLLG PSRTSVARTP RPRPFLPSQE PSMGSRHQRL PRTSHLRAPE
410 420 430 440 450
EQQHLAVSPH PYAQGWGLNR LRCTSQHPAA CPVALRRARP HESSGSGESY
460 470 480 490 500
CTERSGYLAD GPASDSSSGP CHGSSSDSVV NCTDVSLQGI HGSSSTFRSS
510 520 530 540 550
LSSDFDPLVY CSPEGDLQGK GIQPSVTSRP RSLDSVVPRG ETQVSSHIHY
560 570 580 590 600
HRHRHHHYKR QFQWHGRKPG PETGIPQSMP AASHTQLEPS LPDQQLITPN
610 620 630 640 650
PTASSMLPNP QRPRALTEPA PGLAEASSPS PSPKPNPSGL LNLQKSSLTV
660 670 680 690 700
RHPHRKRRGG PSEPLPTSLP PDLTVHTACP VFPHYSPRLA YPWPPEVHPL
710 720 730 740 750
MFRPPGPDRR LLHEVPGPCY SSSQPVWLYL NPCQPLGPCL PGEGHSKWTF
760 770 780
DSPEGRRCPY SHCQVLPAQP GSEEELEELC EQAV
Length:784
Mass (Da):86,304
Last modified:February 1, 2005 - v1
Checksum:i53D4DBE8EE43C755
GO
Isoform 2 (identifier: Q5NCP0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: Missing.

Show »
Length:657
Mass (Da):72,655
Checksum:i49EC496C3981A722
GO
Isoform 3 (identifier: Q5NCP0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-116: PRRAP → QRSTL
     117-784: Missing.

Note: No experimental confirmation available.
Show »
Length:116
Mass (Da):12,491
Checksum:i27523548EAABED79
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PWJ5E9PWJ5_MOUSE
E3 ubiquitin-protein ligase RNF43
Rnf43
743Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti367A → S in AAH75707 (PubMed:15489334).Curated1
Sequence conflicti631P → PSP in AAH75707 (PubMed:15489334).Curated1
Sequence conflicti755G → D in AAH75707 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0232031 – 127Missing in isoform 2. 1 PublicationAdd BLAST127
Alternative sequenceiVSP_023204112 – 116PRRAP → QRSTL in isoform 3. 1 Publication5
Alternative sequenceiVSP_023205117 – 784Missing in isoform 3. 1 PublicationAdd BLAST668

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028750 mRNA Translation: BAC26097.1
AK032782 mRNA Translation: BAC28018.1
AL596086, AL604022 Genomic DNA Translation: CAI35141.1
AL604022, AL596086 Genomic DNA Translation: CAI35962.1
CU393486 Genomic DNA Translation: CAQ52071.1
BC029717 mRNA Translation: AAH29717.1
BC075707 mRNA Translation: AAH75707.1
CCDSiCCDS25215.2 [Q5NCP0-1]
RefSeqiNP_766036.2, NM_172448.3 [Q5NCP0-1]
XP_006532764.1, XM_006532701.3
XP_011247146.1, XM_011248844.2 [Q5NCP0-1]
UniGeneiMm.440230

Genome annotation databases

EnsembliENSMUST00000040089; ENSMUSP00000044241; ENSMUSG00000034177 [Q5NCP0-2]
ENSMUST00000092800; ENSMUSP00000090476; ENSMUSG00000034177 [Q5NCP0-1]
ENSMUST00000165679; ENSMUSP00000130685; ENSMUSG00000034177 [Q5NCP0-1]
GeneIDi207742
KEGGimmu:207742
UCSCiuc007kue.2 mouse [Q5NCP0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028750 mRNA Translation: BAC26097.1
AK032782 mRNA Translation: BAC28018.1
AL596086, AL604022 Genomic DNA Translation: CAI35141.1
AL604022, AL596086 Genomic DNA Translation: CAI35962.1
CU393486 Genomic DNA Translation: CAQ52071.1
BC029717 mRNA Translation: AAH29717.1
BC075707 mRNA Translation: AAH75707.1
CCDSiCCDS25215.2 [Q5NCP0-1]
RefSeqiNP_766036.2, NM_172448.3 [Q5NCP0-1]
XP_006532764.1, XM_006532701.3
XP_011247146.1, XM_011248844.2 [Q5NCP0-1]
UniGeneiMm.440230

3D structure databases

ProteinModelPortaliQ5NCP0
SMRiQ5NCP0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59915N
IntActiQ5NCP0, 1 interactor
STRINGi10090.ENSMUSP00000090476

PTM databases

PhosphoSitePlusiQ5NCP0

Proteomic databases

PaxDbiQ5NCP0
PRIDEiQ5NCP0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040089; ENSMUSP00000044241; ENSMUSG00000034177 [Q5NCP0-2]
ENSMUST00000092800; ENSMUSP00000090476; ENSMUSG00000034177 [Q5NCP0-1]
ENSMUST00000165679; ENSMUSP00000130685; ENSMUSG00000034177 [Q5NCP0-1]
GeneIDi207742
KEGGimmu:207742
UCSCiuc007kue.2 mouse [Q5NCP0-1]

Organism-specific databases

CTDi54894
MGIiMGI:2442609 Rnf43

Phylogenomic databases

eggNOGiKOG0800 Eukaryota
ENOG41121N2 LUCA
GeneTreeiENSGT00760000119057
HOGENOMiHOG000246992
HOVERGENiHBG093916
InParanoidiQ5NCP0
KOiK15694
OrthoDBiEOG091G01HM
PhylomeDBiQ5NCP0
TreeFamiTF317074

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-4641263 Regulation of FZD by ubiquitination

Miscellaneous databases

PROiPR:Q5NCP0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034177 Expressed in 127 organ(s), highest expression level in cochlea
CleanExiMM_RNF43
ExpressionAtlasiQ5NCP0 baseline and differential
GenevisibleiQ5NCP0 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF13639 zf-RING_2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNF43_MOUSE
AccessioniPrimary (citable) accession number: Q5NCP0
Secondary accession number(s): B2KGH3
, Q6DI76, Q8BME0, Q8C191, Q8K0X4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 1, 2005
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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