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Protein

XIAP-associated factor 1

Gene

Xaf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 80TRAF-typeAdd BLAST59

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
XIAP-associated factor 1
Alternative name(s):
BIRC4-binding protein
Gene namesi
Name:Xaf1
Synonyms:Birc4bp, Xiapaf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:3772572 Xaf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003290291 – 273XIAP-associated factor 1Add BLAST273

Proteomic databases

PaxDbiQ5NBU8
PeptideAtlasiQ5NBU8
PRIDEiQ5NBU8

PTM databases

iPTMnetiQ5NBU8
PhosphoSitePlusiQ5NBU8

Expressioni

Gene expression databases

BgeeiENSMUSG00000040483 Expressed in 171 organ(s), highest expression level in decidua
ExpressionAtlasiQ5NBU8 baseline and differential
GenevisibleiQ5NBU8 MM

Interactioni

Subunit structurei

Interacts with BIRC1, BIRC2, BIRC3, BIRC4, BIRC7 and BIRC8. Part of an complex consisting of BIRC4, XAF1 and BIRC5; the complex formation requires IFN-beta stimulation. Interacts with RNF114, the interaction increases XAF1 stability and proapoptotic effects, and may regulate IFN signaling (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000123011

Structurei

3D structure databases

ProteinModelPortaliQ5NBU8
SMRiQ5NBU8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 80TRAF-typeAdd BLAST59

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IK96 Eukaryota
ENOG4110HW5 LUCA
GeneTreeiENSGT00530000063869
HOGENOMiHOG000154869
HOVERGENiHBG108681
InParanoidiQ5NBU8
OMAiCAMCQQS
OrthoDBiEOG091G0OSL
PhylomeDBiQ5NBU8
TreeFamiTF331416

Family and domain databases

InterProiView protein in InterPro
IPR031220 XAF1
PANTHERiPTHR16295:SF17 PTHR16295:SF17, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5NBU8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEADFQVCRN CKRNVASLHF MLHEAHCLRF IVLCPECEEP IPESKMKEHM
60 70 80 90 100
EVVHQQTKES QQHPAKCKFC ELAVQLSNLD VHESHCGSRT EHCPHCNQPI
110 120 130 140 150
TLQVLSQHKA MCLSAKGRPE EGKRIVSSPG RKTRCDLCKQ MIPENTYASH
160 170 180 190 200
MKQCSAPNTV TRIRDESIIV IPSTLAFMDS GNRRSTVSKD VRPKTKNRNS
210 220 230 240 250
STKRETKKQN GTVALPLKSG LQQRADLPTG DETAYDTLQN CCQCRILLPL
260 270
PILNEHQEKC QRLAHQKKLQ WGW
Length:273
Mass (Da):31,117
Last modified:April 8, 2008 - v3
Checksum:i89D504585DACEFCA
GO
Isoform 2 (identifier: Q5NBU8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     57-151: Missing.

Note: Gene prediction based on EST data.
Show »
Length:178
Mass (Da):20,465
Checksum:i0BE1122DADB70663
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Z4YN17Z4YN17_MOUSE
XIAP-associated factor 1
Xaf1
207Annotation score:
F6ZMN8F6ZMN8_MOUSE
XIAP-associated factor 1
Xaf1
75Annotation score:

Sequence cautioni

The sequence BAE24328 differs from that shown. Probable cloning artifact.Curated
The sequence CAI36034 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03971557 – 151Missing in isoform 2. CuratedAdd BLAST95

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL929071 Genomic DNA Translation: CAI36033.2
AL929071 Genomic DNA Translation: CAI36034.1 Sequence problems.
AL929071 Genomic DNA Translation: CAX15644.1
AK140315 mRNA Translation: BAE24328.1 Sequence problems.
CCDSiCCDS24984.2 [Q5NBU8-1]
CCDS70236.1 [Q5NBU8-3]
RefSeqiNP_001032802.2, NM_001037713.4 [Q5NBU8-1]
NP_001278082.1, NM_001291153.1 [Q5NBU8-3]
UniGeneiMm.291131

Genome annotation databases

EnsembliENSMUST00000140842; ENSMUSP00000121472; ENSMUSG00000040483 [Q5NBU8-3]
ENSMUST00000146233; ENSMUSP00000123011; ENSMUSG00000040483 [Q5NBU8-1]
GeneIDi327959
KEGGimmu:327959
UCSCiuc007jyr.2 mouse [Q5NBU8-1]
uc056ymf.1 mouse [Q5NBU8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL929071 Genomic DNA Translation: CAI36033.2
AL929071 Genomic DNA Translation: CAI36034.1 Sequence problems.
AL929071 Genomic DNA Translation: CAX15644.1
AK140315 mRNA Translation: BAE24328.1 Sequence problems.
CCDSiCCDS24984.2 [Q5NBU8-1]
CCDS70236.1 [Q5NBU8-3]
RefSeqiNP_001032802.2, NM_001037713.4 [Q5NBU8-1]
NP_001278082.1, NM_001291153.1 [Q5NBU8-3]
UniGeneiMm.291131

3D structure databases

ProteinModelPortaliQ5NBU8
SMRiQ5NBU8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000123011

PTM databases

iPTMnetiQ5NBU8
PhosphoSitePlusiQ5NBU8

Proteomic databases

PaxDbiQ5NBU8
PeptideAtlasiQ5NBU8
PRIDEiQ5NBU8

Protocols and materials databases

DNASUi327959
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000140842; ENSMUSP00000121472; ENSMUSG00000040483 [Q5NBU8-3]
ENSMUST00000146233; ENSMUSP00000123011; ENSMUSG00000040483 [Q5NBU8-1]
GeneIDi327959
KEGGimmu:327959
UCSCiuc007jyr.2 mouse [Q5NBU8-1]
uc056ymf.1 mouse [Q5NBU8-3]

Organism-specific databases

CTDi54739
MGIiMGI:3772572 Xaf1

Phylogenomic databases

eggNOGiENOG410IK96 Eukaryota
ENOG4110HW5 LUCA
GeneTreeiENSGT00530000063869
HOGENOMiHOG000154869
HOVERGENiHBG108681
InParanoidiQ5NBU8
OMAiCAMCQQS
OrthoDBiEOG091G0OSL
PhylomeDBiQ5NBU8
TreeFamiTF331416

Miscellaneous databases

PROiPR:Q5NBU8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040483 Expressed in 171 organ(s), highest expression level in decidua
ExpressionAtlasiQ5NBU8 baseline and differential
GenevisibleiQ5NBU8 MM

Family and domain databases

InterProiView protein in InterPro
IPR031220 XAF1
PANTHERiPTHR16295:SF17 PTHR16295:SF17, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiXAF1_MOUSE
AccessioniPrimary (citable) accession number: Q5NBU8
Secondary accession number(s): B7ZD14
, Q3USK3, Q5NBU6, Q5NBU7, Q5NBU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: November 7, 2018
This is version 96 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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