UniProtKB - Q5MYT7 (OAS3_RAT)
Protein
2'-5'-oligoadenylate synthase 3
Gene
Oas3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L (By similarity).By similarity
Catalytic activityi
- EC:2.7.7.84
Cofactori
Mg2+Curated
Activity regulationi
Produced as a latent enzyme which is activated by dsRNA generated during the course of viral infection. Strongly activated by long dsRNAs at least 50 nucleotides in length. ssRNA does not activate the enzyme.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 854 | ATPBy similarity | 1 | |
Metal bindingi | 866 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 868 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 938 | Magnesium; catalyticBy similarity | 1 | |
Binding sitei | 997 | ATPBy similarity | 1 | |
Binding sitei | 1000 | ATPBy similarity | 1 | |
Binding sitei | 1019 | ATPBy similarity | 1 |
GO - Molecular functioni
- 2'-5'-oligoadenylate synthetase activity Source: UniProtKB
- ATP binding Source: UniProtKB
- double-stranded RNA binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- defense response to virus Source: RGD
- innate immune response Source: UniProtKB-KW
- negative regulation of viral genome replication Source: RGD
- regulation of ribonuclease activity Source: RGD
- response to virus Source: UniProtKB
Keywordsi
Molecular function | Nucleotidyltransferase, RNA-binding, Transferase |
Biological process | Antiviral defense, Immunity, Innate immunity |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 2'-5'-oligoadenylate synthase 3 (EC:2.7.7.84)Short name: (2-5')oligo(A) synthase 3 Short name: 2-5A synthase 3 |
Gene namesi | Name:Oas3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1359319, Oas3 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleoplasm Source: GO_Central
Other locations
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418631 | 1 – 1137 | 2'-5'-oligoadenylate synthase 3Add BLAST | 1137 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q5MYT7 |
PRIDEi | Q5MYT7 |
Interactioni
Subunit structurei
Monomer.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 154 | Interaction with dsRNABy similarity | 1 | |
Sitei | 242 | Interaction with dsRNABy similarity | 1 |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000066596 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 6 – 341 | OAS domain 1CuratedAdd BLAST | 336 | |
Regioni | 12 – 56 | Interaction with dsRNABy similarityAdd BLAST | 45 | |
Regioni | 185 – 199 | Interaction with dsRNABy similarityAdd BLAST | 15 | |
Regioni | 342 – 461 | LinkerCuratedAdd BLAST | 120 | |
Regioni | 462 – 792 | OAS domain 2CuratedAdd BLAST | 331 | |
Regioni | 800 – 1134 | OAS domain 3CuratedAdd BLAST | 335 |
Domaini
OAS domain 3 is catalytically active. OAS domain 1 has no catalytic activity but is essential for recognition of long dsRNAs.By similarity
Sequence similaritiesi
Belongs to the 2-5A synthase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502S649, Eukaryota |
InParanoidi | Q5MYT7 |
OrthoDBi | 611234at2759 |
PhylomeDBi | Q5MYT7 |
Family and domain databases
CDDi | cd05400, NT_2-5OAS_ClassI-CCAase, 3 hits |
Gene3Di | 1.10.1410.20, 3 hits 3.30.460.10, 3 hits |
InterProi | View protein in InterPro IPR018952, 2-5-oligoAdlate_synth_1_dom2/C IPR038121, 2-5-oligoAdlate_synth_2_sf IPR026774, 2-5A_synthase IPR006117, 2-5OAS_C_CS IPR043518, 2-5OAS_N_CS IPR006116, NT_2-5OAS_ClassI-CCAase IPR043519, NT_sf IPR002934, Polymerase_NTP_transf_dom |
PANTHERi | PTHR11258, PTHR11258, 2 hits |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF10421, OAS1_C, 3 hits |
SUPFAMi | SSF81301, SSF81301, 3 hits |
PROSITEi | View protein in PROSITE PS00832, 25A_SYNTH_1, 1 hit PS00833, 25A_SYNTH_2, 2 hits PS50152, 25A_SYNTH_3, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q5MYT7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLYHTPAGA LDKLVAHSLH PAPEFTAAVR RALGSLDNVL RKNGAGGLQR
60 70 80 90 100
PRVIRIIKGG AHARGTALRG GTDVELVIFL DCLRSFGDQK TCHTEILGAI
110 120 130 140 150
QALLESWGCN PGPGLTFEFS GPKASGILQF RLASVDQENW IDVSLVPAFD
160 170 180 190 200
ALGQLHSEVK PTPNVYSSLL SSHCQAGEHS ACFTELRKNF VNIRPVKLKN
210 220 230 240 250
LILLVKHWYR QVQTQVVRAT LPPSYALELL TIFAWEQGCR KDAFSLAQGL
260 270 280 290 300
RTVLALIQRN KHLCIFWTEN YGFEDPAVGE FLRRQLKRPR PVILDPADPT
310 320 330 340 350
WDLGNGTAWC WDVLAKEAEY SFNQQCFKEA SGALVQPWEG PGLPCAGILD
360 370 380 390 400
LGHPIQQGAK HALEDNNGHL AVQPMKESLQ PSNPARGLPE TATKISAMPD
410 420 430 440 450
PTVTETHKSL KKSVHPKTVS ETVVNPSSHV WITQSTASSN TPPGHSSMST
460 470 480 490 500
AGSQMGPDLS QIPSKELDSF IQDHLRPSSQ FQQQVRQAID TILCCLREKC
510 520 530 540 550
VDKVLRVSKG GSFGRGTDLR GKCDVELVIF YKTLGDFKGQ NSHQTEILCD
560 570 580 590 600
MQAQLQRWCQ NPAPGLSLQF IEQKSNALHL QLVPTNLSNR VDLSVLPAFD
610 620 630 640 650
AVGPLKSGAK PLPETYSSLL SSGCQAGEHA ACFAELRRNF INTRPAKLRS
660 670 680 690 700
LMLLVKHWYR QVAARFEGGE TAGAALPPAY ALELLTVFAW EQGCGEQKFS
710 720 730 740 750
MAEGLRTVLR LVQQHQSLCI YWTVNYSVQD PAIRAHLLRQ LRKARPLILD
760 770 780 790 800
PADPTWNMDQ GNWKLLAQEA AALESQVCLQ SRDGNLVPPW DVMPALLHQT
810 820 830 840 850
PAQNLDKFIC EFLQPDRHFL TQVKRAVDTI CSFLKENCFR NSTIKVLKVV
860 870 880 890 900
KGGSSAKGTA LQGRSDADLV VFLSCFRQFS EQGSHRAEII AEIQAQLEAC
910 920 930 940 950
QQKQRFDVKF EISKRKNPRV LSFTLTSKTL LGQSVDFDVL PAFDALGQLK
960 970 980 990 1000
SGSRPDPRVY TDLIQSYSNA GEFSTCFTEL QRDFISSRPT KLKSLIRLVK
1010 1020 1030 1040 1050
HWYQQCNKTV KGKGSLPPQH GLELLTVYAW ERGSQNPQFN MAEGFRTVLE
1060 1070 1080 1090 1100
LIGQYRQLCV YWTINYGAED ETIGDFLKMQ LQKPRPVILD PADPTGNLGH
1110 1120 1130
NARWDLLAKE AAAYTSALCC MDKDGNPIKP WPVKAAV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K4J7 | A0A0G2K4J7_RAT | 2'-5' oligoadenylate synthase | Oas3 | 1,005 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY250706 mRNA Translation: AAP76224.1 |
RefSeqi | NP_001009493.1, NM_001009493.1 |
Genome annotation databases
GeneIDi | 494202 |
KEGGi | rno:494202 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY250706 mRNA Translation: AAP76224.1 |
RefSeqi | NP_001009493.1, NM_001009493.1 |
3D structure databases
SMRi | Q5MYT7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000066596 |
Proteomic databases
PaxDbi | Q5MYT7 |
PRIDEi | Q5MYT7 |
Genome annotation databases
GeneIDi | 494202 |
KEGGi | rno:494202 |
Organism-specific databases
CTDi | 4940 |
RGDi | 1359319, Oas3 |
Phylogenomic databases
eggNOGi | ENOG502S649, Eukaryota |
InParanoidi | Q5MYT7 |
OrthoDBi | 611234at2759 |
PhylomeDBi | Q5MYT7 |
Miscellaneous databases
PROi | PR:Q5MYT7 |
Family and domain databases
CDDi | cd05400, NT_2-5OAS_ClassI-CCAase, 3 hits |
Gene3Di | 1.10.1410.20, 3 hits 3.30.460.10, 3 hits |
InterProi | View protein in InterPro IPR018952, 2-5-oligoAdlate_synth_1_dom2/C IPR038121, 2-5-oligoAdlate_synth_2_sf IPR026774, 2-5A_synthase IPR006117, 2-5OAS_C_CS IPR043518, 2-5OAS_N_CS IPR006116, NT_2-5OAS_ClassI-CCAase IPR043519, NT_sf IPR002934, Polymerase_NTP_transf_dom |
PANTHERi | PTHR11258, PTHR11258, 2 hits |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF10421, OAS1_C, 3 hits |
SUPFAMi | SSF81301, SSF81301, 3 hits |
PROSITEi | View protein in PROSITE PS00832, 25A_SYNTH_1, 1 hit PS00833, 25A_SYNTH_2, 2 hits PS50152, 25A_SYNTH_3, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OAS3_RAT | |
Accessioni | Q5MYT7Primary (citable) accession number: Q5MYT7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | February 1, 2005 | |
Last modified: | April 7, 2021 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families