UniProtKB - Q5MIB6 (PYGB_SHEEP)
Protein
Glycogen phosphorylase, brain form
Gene
PYGB
Organism
Ovis aries (Sheep)
Status
Functioni
Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.By similarity
Catalytic activityi
- EC:2.4.1.1
Cofactori
pyridoxal 5'-phosphateBy similarity
Activity regulationi
Activity of phosphorylase is controlled both by allosteric means (through the non-covalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose-6-phosphate allosterically inhibit, phosphorylase B (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 43 | AMP; shared with dimeric partnerBy similarity | 1 | |
Sitei | 76 | Participates in a stacking interaction with the adenine ring of AMPBy similarity | 1 | |
Sitei | 109 | Involved in the association of subunitsBy similarity | 1 | |
Sitei | 143 | Involved in the association of subunitsBy similarity | 1 | |
Sitei | 156 | May be involved in allosteric controlBy similarity | 1 | |
Binding sitei | 197 | AMPBy similarity | 1 | |
Binding sitei | 310 | AMPBy similarity | 1 | |
Binding sitei | 569 | Pyridoxal 5'-phosphateBy similarity | 1 |
GO - Molecular functioni
- glycogen phosphorylase activity Source: InterPro
- linear malto-oligosaccharide phosphorylase activity Source: UniProtKB-EC
- pyridoxal phosphate binding Source: InterPro
- SHG alpha-glucan phosphorylase activity Source: UniProtKB-EC
GO - Biological processi
- glycogen metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Allosteric enzyme, Glycosyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Glycogen metabolism |
Ligand | Pyridoxal phosphate |
Protein family/group databases
CAZyi | GT35, Glycosyltransferase Family 35 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PYGB |
Organismi | Ovis aries (Sheep) |
Taxonomic identifieri | 9940 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000188539 | 2 – 843 | Glycogen phosphorylase, brain formAdd BLAST | 842 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 15 | Phosphoserine; by PHK; in form phosphorylase ABy similarity | 1 | |
Modified residuei | 197 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 473 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 681 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Post-translational modificationi
Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PRIDEi | Q5MIB6 |
Interactioni
Subunit structurei
Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9940.ENSOARP00000007688 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 677 – 678 | Pyridoxal 5'-phosphateBy similarity | 2 |
Sequence similaritiesi
Belongs to the glycogen phosphorylase family.Curated
Phylogenomic databases
eggNOGi | KOG2099, Eukaryota |
OrthoDBi | 240595at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR011833, Glycg_phsphrylas IPR000811, Glyco_trans_35 IPR035090, Pyridoxal_P_attach_site |
PANTHERi | PTHR11468, PTHR11468, 1 hit |
Pfami | View protein in Pfam PF00343, Phosphorylase, 1 hit |
PIRSFi | PIRSF000460, Pprylas_GlgP, 1 hit |
TIGRFAMsi | TIGR02093, P_ylase, 1 hit |
PROSITEi | View protein in PROSITE PS00102, PHOSPHORYLASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5MIB6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKPLTDGER RKQISVRGLA GLGDVAEVRK SFNRHLHFTL VKDRNVATRR
60 70 80 90 100
DYYLALAHTV RDHLVGRWIR TQQRYYERDP KRIYYLSLEF YMGRTLQNTM
110 120 130 140 150
VNLGLQNACD EAIYQLGLDL EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEFGIFNQK IVNGWQVEEA DDWLRYGNPW EKARPEYMLP
210 220 230 240 250
VHFYGRVEHS PEGVRWLDTQ VVLAMPYDTP VPGYKNDTVN TMRLWSAKAP
260 270 280 290 300
NDFKLHDFNV GGYIEAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDIIR RFKSSKFGCR DPVRTSFETF PDKVAIQLND THPALAIPEL
360 370 380 390 400
MRILVDVEKV DWGKAWEITK KTCAYTNHTV LPEALERWPV SMFEKLLPRH
410 420 430 440 450
LDIIYAINQR HLDHVAALFP GDVDRLRRMS VIEEGDCKRI NMAHLCVIGS
460 470 480 490 500
HAVNGVARIH SEIVRQSVFK DFYELEPEKF QNKTNGITPR RWLLLCNPGL
510 520 530 540 550
AETIVERIGE DFLTDLSQLK KLLPLVGDEA LIRDVAQVKQ ENKVKFSAFL
560 570 580 590 600
EKQYGVKVNP SSMFDVHVKR IHEYKRQLLN CLHVVTLYNR IKKDPTQAFV
610 620 630 640 650
PRTVMIGGKA APGYHMAKKI IKLVTSIGDI VNHDPIVGDR LKVIFLENYR
660 670 680 690 700
VSLAEKVIPA ADLSQQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGAENLF IFGLRVEDVE ALDRKGYNAH EYYNHLPELQ QAVDQINSGF
760 770 780 790 800
FSPREPDCFK DVVNMLLNHD RFKVFADYEA YVACQAQVDQ LYRNPKEWTK
810 820 830 840
KVIRNIACSG KFSSDRTITE YARDIWGAEP PALQTPPPSL PRD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY827550 mRNA Translation: AAV87308.1 |
RefSeqi | NP_001020034.1, NM_001024863.1 |
Genome annotation databases
GeneIDi | 554325 |
KEGGi | oas:554325 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY827550 mRNA Translation: AAV87308.1 |
RefSeqi | NP_001020034.1, NM_001024863.1 |
3D structure databases
SMRi | Q5MIB6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9940.ENSOARP00000007688 |
Protein family/group databases
CAZyi | GT35, Glycosyltransferase Family 35 |
Proteomic databases
PRIDEi | Q5MIB6 |
Genome annotation databases
GeneIDi | 554325 |
KEGGi | oas:554325 |
Organism-specific databases
CTDi | 5834 |
Phylogenomic databases
eggNOGi | KOG2099, Eukaryota |
OrthoDBi | 240595at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR011833, Glycg_phsphrylas IPR000811, Glyco_trans_35 IPR035090, Pyridoxal_P_attach_site |
PANTHERi | PTHR11468, PTHR11468, 1 hit |
Pfami | View protein in Pfam PF00343, Phosphorylase, 1 hit |
PIRSFi | PIRSF000460, Pprylas_GlgP, 1 hit |
TIGRFAMsi | TIGR02093, P_ylase, 1 hit |
PROSITEi | View protein in PROSITE PS00102, PHOSPHORYLASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PYGB_SHEEP | |
Accessioni | Q5MIB6Primary (citable) accession number: Q5MIB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 71 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families