Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vascular endothelial growth factor receptor 3

Gene

flt4

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Tyrosine-protein kinase that acts as a cell-surface receptor for vegf or vegfc. Combinations of multiple VEGF receptors are required for development of different blood vessel types in the embryo. Involved in angiogenesis, specifically in VEGF-induced sprouting of new blood vessels, but not required for proper vasculogenesis or hematopoiesis.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Activity regulationi

Present in an inactive conformation in the absence of bound ligand. Binding of vegfc or vegfd leads to dimerization and activation by autophosphorylation on tyrosine residues (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei900ATPPROSITE-ProRule annotation1
Active sitei1045Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi872 – 880ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein homodimerization activity Source: ZFIN
  • transmembrane receptor protein tyrosine kinase activity Source: GO_Central
  • transmembrane signaling receptor activity Source: GO_Central
  • VEGF-C-activated receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase
Biological processAngiogenesis, Differentiation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vascular endothelial growth factor receptor 3 (EC:2.7.10.1)
Short name:
VEGFR-3
Gene namesi
Name:flt4
Synonyms:flt-4, vegfr3
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-980526-326 flt4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 796ExtracellularSequence analysisAdd BLAST772
Transmembranei797 – 817HelicalSequence analysisAdd BLAST21
Topological domaini818 – 1357CytoplasmicSequence analysisAdd BLAST540

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000024946425 – 1357Vascular endothelial growth factor receptor 3Add BLAST1333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi44N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi48N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi51 ↔ 121PROSITE-ProRule annotation
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi173 ↔ 225PROSITE-ProRule annotation
Glycosylationi216N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi271N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi272 ↔ 331PROSITE-ProRule annotation
Glycosylationi360N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi400N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi440N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi473 ↔ 562PROSITE-ProRule annotation
Disulfide bondi493 ↔ 514By similarity
Glycosylationi553N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi606 ↔ 674PROSITE-ProRule annotation
Glycosylationi610N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi660N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi707N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi711N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi720 ↔ 772PROSITE-ProRule annotation
Glycosylationi751N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1071Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1076Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1226Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1227Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1334Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1338Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5MD89
PRIDEiQ5MD89

Interactioni

Subunit structurei

Interacts with vegfc and vegfd. Monomer in the absence of bound vegfc or vegfd. Homodimer in the presence of bound vegfc or vegfd (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000112456

Structurei

3D structure databases

ProteinModelPortaliQ5MD89
SMRiQ5MD89
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 121Ig-like C2-type 1Add BLAST97
Domaini138 – 244Ig-like C2-type 2Add BLAST107
Domaini255 – 343Ig-like C2-type 3Add BLAST89
Domaini352 – 442Ig-like C2-type 4Add BLAST91
Domaini453 – 583Ig-like C2-type 5Add BLAST131
Domaini583 – 690Ig-like C2-type 6Add BLAST108
Domaini699 – 785Ig-like C2-type 7Add BLAST87
Domaini866 – 1181Protein kinasePROSITE-ProRule annotationAdd BLAST316

Domaini

The first and second Ig-like C2-type (immunoglobulin-like) domains are sufficient for VEGFC binding. The fourth and fifth Ig-like C2-type (immunoglobulin-like) domains are sufficient for homodimerization. The fifth and seventh Ig-like C2-type (immunoglobulin-like) domains are required for autophosphorylation and further activation.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0200 Eukaryota
COG0515 LUCA
HOGENOMiHOG000037949
HOVERGENiHBG053432
InParanoidiQ5MD89
PhylomeDBiQ5MD89

Family and domain databases

Gene3Di2.60.40.10, 7 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR001824 Tyr_kinase_rcpt_3_CS
IPR009137 VEGFR3_rcpt
PANTHERiPTHR24416:SF49 PTHR24416:SF49, 1 hit
PfamiView protein in Pfam
PF07679 I-set, 2 hits
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00409 IG, 6 hits
SM00408 IGc2, 6 hits
SM00219 TyrKc, 1 hit
SUPFAMiSSF48726 SSF48726, 6 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 5 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS00240 RECEPTOR_TYR_KIN_III, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q5MD89-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKRDFTFFCR IWIGIPFFSG LVNGFSMSPP TLDNTKDQLV INANDTLNIT
60 70 80 90 100
CRGQRILDWS WPEESLSKVE FTDRQGQQSP TDTPGYREIR LKECQGVAGK
110 120 130 140 150
PYCKILILTN AQANDSGYYR CFYKDIKAVI DGTTAASIFV FVRDPEHPFI
160 170 180 190 200
KRGDNDMETI FITDSETHIE VPCLVSDPDL KVTLFSLVPY PEPVDGSVVT
210 220 230 240 250
WNNKKGWSIP RHIIQNTSTF IGFYCSISVQ NSQHTSSIYV VQVIGLKFYE
260 270 280 290 300
FKLFPEDSPV ELMQGESLVL NCTALVDFNT GVDFQWDYPG KKENRLASLQ
310 320 330 340 350
PLRNVLDEAT EISSILSIRN IHLDDSGYYT CWANTLEMKR ELTTVVIVHE
360 370 380 390 400
KPFISLDYRN GSVIEAKEGQ KSVRLSVKVS AYPSPEIQWY KNGKLISSKN
410 420 430 440 450
SSRFKVQQHS LQIRDVCKQD AGEYMLVLKN SPAALEKRLN FTLIVNVPPQ
460 470 480 490 500
IHEKEAAPPT NLYGKGTRQI LTCTADGSPP ASISWQWRPW SPCDLERTRR
510 520 530 540 550
ALRRRGGRDQ SPFCHNWMDL DPEHAVNPIE SIDTLTQMVD GKEKTVGRVV
560 570 580 590 600
IQNASVPAMY KCLAENRVGK DERLIYFYVT TIPEGFDIEM EPSEDPLEQD
610 620 630 640 650
LVQLKCNADN FTYENLRWYR LDPQTVPPEL DCKSLHQYAT FLEGQLSFQT
660 670 680 690 700
TSNNWVLQLN ITNIQLQDEG NYVCEVQNRR TGVKHCHRKY IPVKAMEAPR
710 720 730 740 750
YRHNPTNHTV NVSESLQMNC DVEGTPFPQL SWFKDNQPLH QISGILLQDS
760 770 780 790 800
NRTLSIQRVR EEDAGLYTCS ACNQKGCVQS SATVSVIGSD DKTNVEIVIL
810 820 830 840 850
IGTGVIAIFF WVLLLVIFCN VKRVNPADIK TGYLSIIMDP GEVPLEEQCE
860 870 880 890 900
YLPYDSSQWE ISRDRLRLGK VLGHGAFGKV IEASIFGHDK KSSANTVAVK
910 920 930 940 950
MLKEGATASE HKALMSELKI LIHIGNHLNV VNLLGACTKP NGPLMVIVEY
960 970 980 990 1000
CKYGNLSNFL RAKREFFLPY RDRSPKTQSQ VRRMIEAGQA SQSEHQPSTS
1010 1020 1030 1040 1050
STNPPRVTVD DLWKTPLTIE DLICYSFQVA RGMEFLASRK CIHRDLAARN
1060 1070 1080 1090 1100
ILLSENNVVK ICDFGLARDI YKDPDYVRKG NARLPLKWMA PESIFDKVYT
1110 1120 1130 1140 1150
SQSDVWSFGV LLWEIFSLGA SPYPGIQIDE DFCKRLKDGT RMRAPDNASP
1160 1170 1180 1190 1200
EIYGIMLACW QGEPRERPTF PALVEILGDL LQENSLPEIP FNVSQSSEDD
1210 1220 1230 1240 1250
GFSQASSRPP SQEEIRLACN TLPTRYYNCV PFAGCVMVGP SSTCHSRVKT
1260 1270 1280 1290 1300
FEELPMEMTS HKTQHDSQTD SGMVLASDEL ERFEHKHRGA MLTTATTGQS
1310 1320 1330 1340 1350
TDRLISCPSV SSSGSGGGLL RPVFFTQLSG QTFYNNEYGH LSEEGVSDYF

SSSDQAV
Length:1,357
Mass (Da):153,178
Last modified:February 1, 2005 - v1
Checksum:iD68C197828BE278E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4IFS9A0A0R4IFS9_DANRE
Vascular endothelial growth factor ...
flt4
1,368Annotation score:
A0A2R8QSE0A0A2R8QSE0_DANRE
Vascular endothelial growth factor ...
flt4
1,324Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti567R → K in AAS92272 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY524001 mRNA Translation: AAS92272.1
AY833404 mRNA Translation: AAV93318.1
UniGeneiDr.81298

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY524001 mRNA Translation: AAS92272.1
AY833404 mRNA Translation: AAV93318.1
UniGeneiDr.81298

3D structure databases

ProteinModelPortaliQ5MD89
SMRiQ5MD89
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000112456

Proteomic databases

PaxDbiQ5MD89
PRIDEiQ5MD89

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ZFINiZDB-GENE-980526-326 flt4

Phylogenomic databases

eggNOGiKOG0200 Eukaryota
COG0515 LUCA
HOGENOMiHOG000037949
HOVERGENiHBG053432
InParanoidiQ5MD89
PhylomeDBiQ5MD89

Miscellaneous databases

PROiPR:Q5MD89

Family and domain databases

Gene3Di2.60.40.10, 7 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR001824 Tyr_kinase_rcpt_3_CS
IPR009137 VEGFR3_rcpt
PANTHERiPTHR24416:SF49 PTHR24416:SF49, 1 hit
PfamiView protein in Pfam
PF07679 I-set, 2 hits
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00409 IG, 6 hits
SM00408 IGc2, 6 hits
SM00219 TyrKc, 1 hit
SUPFAMiSSF48726 SSF48726, 6 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 5 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS00240 RECEPTOR_TYR_KIN_III, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVGFR3_DANRE
AccessioniPrimary (citable) accession number: Q5MD89
Secondary accession number(s): Q5GIT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: February 1, 2005
Last modified: November 7, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again