UniProtKB - Q5MBR6 (NCED2_ORYSJ)
Protein
9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic
Gene
NCED2
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids.By similarity
Catalytic activityi
- EC:1.13.11.51By similarity
- 9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinBy similarityEC:1.13.11.51By similarity
- 9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinBy similarityEC:1.13.11.51By similarity
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 270 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 319 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 385 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 563 | Iron; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- 9-cis-epoxycarotenoid dioxygenase activity Source: UniProtKB-EC
- carotenoid dioxygenase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- abscisic acid biosynthetic process Source: UniProtKB-KW
- carotene catabolic process Source: GO_Central
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Abscisic acid biosynthesis, Stress response |
Ligand | Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 9-cis-epoxycarotenoid dioxygenase NCED2, chloroplasticCurated (EC:1.13.11.51By similarity)Short name: OsNCED2Imported |
Gene namesi | Name:NCED2Imported Ordered Locus Names:Os12g0435200Imported, LOC_Os12g24800Imported ORF Names:OsJ_35952Imported |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Chloroplast
- chloroplast Sequence analysis
Chloroplast
- chloroplast Source: GO_Central
- chloroplast stroma Source: GO_Central
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 34 | ChloroplastSequence analysisAdd BLAST | 34 | |
ChainiPRO_0000440938 | 35 – 576 | 9-cis-epoxycarotenoid dioxygenase NCED2, chloroplasticAdd BLAST | 542 |
Proteomic databases
PaxDbi | Q5MBR6 |
PRIDEi | Q5MBR6 |
Expressioni
Inductioni
Family & Domainsi
Sequence similaritiesi
Belongs to the carotenoid oxygenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | Q5MBR6 |
OMAi | RINLCTG |
OrthoDBi | 524712at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5MBR6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEVPIAAMTF AHPANVMTLA SRQPKSKRSH ISPATTAHRN LQTRLAHHHH
60 70 80 90 100
ATPASLPMAI CNTVDKVINR FIDLPEQRPT VDPRRVLSGN FAPVDELPPT
110 120 130 140 150
SCHVIRGSIP SCLAGGVYIR NGPNPQHRLP QRTHHLFDGD GMLHSLLIPS
160 170 180 190 200
ASSTLLSEPV LCSRYVHTYK YLLERETGGP VLPNFFAGFH GVAGLARAVV
210 220 230 240 250
MIARVLAGQI NLNKGFGLAN TSITLFADCL YALCESDLPY SMHINPANGE
260 270 280 290 300
VTTLGRCDFG GDLSFRMTAH PKKDPVTMEL FAFRYNVFQP FITYFWFDRA
310 320 330 340 350
GSKVADVPIL SLQKPSVMHD FAITERYAIF PESQLIVNPM DMVMRGSSLV
360 370 380 390 400
GLDRTMVPRI GVLPRYAKDE SDMRWFEVPR FNMLHTTNGW EEADGEEIVL
410 420 430 440 450
VAPNILSIEH MLGNMELMRA RVDMVRINLC TGDVSCTALS PESLEFGVIH
460 470 480 490 500
QGYVGRKNRY GYFGVSGPLP KIKGIRKLDF DLVGSGDCTV GRRDFGLGCF
510 520 530 540 550
AGEPFFVPDN IDGYGNEDSG YVVCYTHEED TGESWFVVMD AKSPELDIVA
560 570
EVQLPSRIPY GFHGIFVKQA ELLAQQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY838898 mRNA Translation: AAW21318.1 DP000011 Genomic DNA Translation: ABA97976.1 AP008218 Genomic DNA Translation: BAF29705.1 AP014968 Genomic DNA Translation: BAT16965.1 CM000149 Genomic DNA Translation: EAZ20344.1 |
RefSeqi | XP_015619611.1, XM_015764125.1 |
Genome annotation databases
EnsemblPlantsi | Os12t0435200-01; Os12t0435200-01; Os12g0435200 |
GeneIDi | 4352125 |
Gramenei | Os12t0435200-01; Os12t0435200-01; Os12g0435200 |
KEGGi | osa:4352125 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY838898 mRNA Translation: AAW21318.1 DP000011 Genomic DNA Translation: ABA97976.1 AP008218 Genomic DNA Translation: BAF29705.1 AP014968 Genomic DNA Translation: BAT16965.1 CM000149 Genomic DNA Translation: EAZ20344.1 |
RefSeqi | XP_015619611.1, XM_015764125.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 4530.OS12T0435200-01 |
Proteomic databases
PaxDbi | Q5MBR6 |
PRIDEi | Q5MBR6 |
Genome annotation databases
EnsemblPlantsi | Os12t0435200-01; Os12t0435200-01; Os12g0435200 |
GeneIDi | 4352125 |
Gramenei | Os12t0435200-01; Os12t0435200-01; Os12g0435200 |
KEGGi | osa:4352125 |
Phylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | Q5MBR6 |
OMAi | RINLCTG |
OrthoDBi | 524712at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCED2_ORYSJ | |
Accessioni | Q5MBR6Primary (citable) accession number: Q5MBR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2017 |
Last sequence update: | February 1, 2005 | |
Last modified: | December 2, 2020 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - SIMILARITY comments
Index of protein domains and families