UniProtKB - Q5MBR5 (NCED3_ORYSJ)
Protein
9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic
Gene
NCED3
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids.By similarity
Catalytic activityi
- EC:1.13.11.51By similarity
- 9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinBy similarityEC:1.13.11.51By similarity
- 9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinBy similarityEC:1.13.11.51By similarity
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 303 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 352 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 417 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 595 | Iron; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- 9-cis-epoxycarotenoid dioxygenase activity Source: UniProtKB-EC
- carotenoid dioxygenase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- abscisic acid biosynthetic process Source: UniProtKB-KW
- carotene catabolic process Source: GO_Central
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Abscisic acid biosynthesis, Stress response |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
PlantReactomei | R-OSA-1119374, Abscisic acid biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplasticCurated (EC:1.13.11.51By similarity)Short name: OsNCED3Imported |
Gene namesi | Name:NCED3Imported Ordered Locus Names:Os03g0645900Imported, LOC_Os03g44380Imported ORF Names:OsJ_11896Imported |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Chloroplast
- chloroplast Sequence analysis
Chloroplast
- chloroplast Source: GO_Central
- chloroplast stroma Source: GO_Central
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 38 | ChloroplastSequence analysisAdd BLAST | 38 | |
ChainiPRO_0000440939 | 39 – 608 | 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplasticAdd BLAST | 570 |
Proteomic databases
PaxDbi | Q5MBR5 |
PRIDEi | Q5MBR5 |
Expressioni
Tissue specificityi
Expressed in vascular bundles of roots and leaves, vascular parenchyma cells of leaves, anther filaments, xylem tissue of anthers, embryos, radicles and coleoptiles.1 Publication
Inductioni
Induced by abscisic acid (ABA) and salt (PubMed:18326788, PubMed:25283804). Induced by drought stress (PubMed:20838584, PubMed:25418692, PubMed:25283804, PubMed:25735958). Induced by D-allose (PubMed:23397192). Induced by infection with the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) (PubMed:23826294, PubMed:26718470). Down-regulated by submergence (PubMed:17205969).9 Publications
Family & Domainsi
Sequence similaritiesi
Belongs to the carotenoid oxygenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | Q5MBR5 |
OMAi | KEDGPPF |
OrthoDBi | 524712at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5MBR5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATITTPGYA HIQRQHGRCS TTAGRRGASN SVRFSARAVS SVPHAAAASS
60 70 80 90 100
APAFLPVPFV PGADAPSPSG KSAIGVPKAP RKGEEGKRLN FFQRAAAMAL
110 120 130 140 150
DAFEEGFVAN VLERPHGLPS TADPAVQIAG NFAPVGETPP ARALPVSGRI
160 170 180 190 200
PPFINGVYAR NGANPHFDPV AGHHLFDGDG MVHAVRIRNG AAESYACRFT
210 220 230 240 250
ETARLRQERA MGRPMFPKAI GELHGHSGIA RLALFYARAA CGLLDPSHGT
260 270 280 290 300
GVANAGLIYF NGRLLAMSED DLPYQVRVTA DGDLETVGRY DFDGQLGCAM
310 320 330 340 350
IAHPKLDPAT GELHALSYDV IKKPYLKYFY FAPDGTKSAD VEIPLDQPTM
360 370 380 390 400
IHDFAITENY VVVPDHQVVF KLQEMLRGGS PVVLDKEKTS RFGVLPKHAA
410 420 430 440 450
DASEMVWVDV PDCFCFHLWN AWEEADTDEV VVIGSCMTPA DSIFNESDDR
460 470 480 490 500
LESVLTEIRL NTRTGESTRR AILPPSSQVN LEVGMVNRNL LGRKTRYAYL
510 520 530 540 550
AVAEPWPKVS GFAKVDLATG ELTKFEYGEG RFGGEPCFVP MDAAAATPRG
560 570 580 590 600
EDDGYILSFV HDERAGTSEL LVVNAADMRL EATVQLPSRV PYGFHGTFIT
GDELTTQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY838899 mRNA Translation: AAW21319.1 DP000009 Genomic DNA Translation: ABF97874.1 AP008209 Genomic DNA Translation: BAF12679.1 AP014959 Genomic DNA Translation: BAS85471.1 CM000140 Genomic DNA Translation: EAZ27934.1 |
RefSeqi | XP_015631538.1, XM_015776052.1 |
Genome annotation databases
EnsemblPlantsi | Os03t0645900-00; Os03t0645900-00; Os03g0645900 |
GeneIDi | 4333566 |
Gramenei | Os03t0645900-00; Os03t0645900-00; Os03g0645900 |
KEGGi | osa:4333566 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY838899 mRNA Translation: AAW21319.1 DP000009 Genomic DNA Translation: ABF97874.1 AP008209 Genomic DNA Translation: BAF12679.1 AP014959 Genomic DNA Translation: BAS85471.1 CM000140 Genomic DNA Translation: EAZ27934.1 |
RefSeqi | XP_015631538.1, XM_015776052.1 |
3D structure databases
SMRi | Q5MBR5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4530.OS03T0645900-00 |
Proteomic databases
PaxDbi | Q5MBR5 |
PRIDEi | Q5MBR5 |
Genome annotation databases
EnsemblPlantsi | Os03t0645900-00; Os03t0645900-00; Os03g0645900 |
GeneIDi | 4333566 |
Gramenei | Os03t0645900-00; Os03t0645900-00; Os03g0645900 |
KEGGi | osa:4333566 |
Phylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | Q5MBR5 |
OMAi | KEDGPPF |
OrthoDBi | 524712at2759 |
Enzyme and pathway databases
PlantReactomei | R-OSA-1119374, Abscisic acid biosynthesis |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCED3_ORYSJ | |
Accessioni | Q5MBR5Primary (citable) accession number: Q5MBR5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2017 |
Last sequence update: | February 1, 2005 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - SIMILARITY comments
Index of protein domains and families