UniProtKB - Q5MBR3 (NCED5_ORYSJ)
Protein
9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic
Gene
NCED5
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids.By similarity
Catalytic activityi
- EC:1.13.11.51By similarity
- 9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinBy similarityEC:1.13.11.51By similarity
- 9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinBy similarityEC:1.13.11.51By similarity
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 305 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 354 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 419 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 600 | Iron; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- 9-cis-epoxycarotenoid dioxygenase activity Source: UniProtKB-EC
- carotenoid dioxygenase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- abscisic acid biosynthetic process Source: UniProtKB-KW
- carotene catabolic process Source: GO_Central
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Abscisic acid biosynthesis, Stress response |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
PlantReactomei | R-OSA-1119374, Abscisic acid biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplasticCurated (EC:1.13.11.51By similarity)Short name: OsNCED5Imported |
Gene namesi | Name:NCED5Imported Ordered Locus Names:Os12g0617400Imported, LOC_Os12g42280Imported |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Chloroplast
- chloroplast Sequence analysis
Chloroplast
- chloroplast Source: GO_Central
- chloroplast stroma Source: GO_Central
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 36 | ChloroplastSequence analysisAdd BLAST | 36 | |
ChainiPRO_0000440941 | 37 – 613 | 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplasticSequence analysisAdd BLAST | 577 |
Proteomic databases
PRIDEi | Q5MBR3 |
Expressioni
Inductioni
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 37 – 104 | Ala-richPROSITE-ProRule annotationAdd BLAST | 68 | |
Compositional biasi | 49 – 69 | Pro-richPROSITE-ProRule annotationAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the carotenoid oxygenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1285, Eukaryota |
InParanoidi | Q5MBR3 |
OrthoDBi | 524712at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5MBR3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPTTFTPNSP ASSCSIHHRA SPSRGARNSV RFTRPRAAAA ATNSVLSAPS
60 70 80 90 100
SVPPAYVPPP PPPPTKMFPE AGDAAAAKAA ARRCGKKKDG LNFFQRAAAV
110 120 130 140 150
ALDAFEEGFI TNVLERPHAL PRTADPAVQI AGNFAPVGEQ PPVRSLPVSG
160 170 180 190 200
RIPPFINGVY ARNGANPHFE PTAGHHLFDG DGMVHAVRIR NGAAESYACR
210 220 230 240 250
FTETARLGQE RALGRAVFPK AIGELHGHSG IARLALFYAR GLCGLVDPSH
260 270 280 290 300
GTGVANAGLV YFNGRLLAMS EDDLPYQVRV TADGDLETVG RYDFDGQLGC
310 320 330 340 350
AMIAHPKLDP VSGELFALSY DVIKKPYLKY FYFDADGTKS PDVEIELEQP
360 370 380 390 400
TMIHDFAITE NFVVVPDHQV VFKLGEMFRG GSPVVLDREK TSRFGVLPKH
410 420 430 440 450
ATSSLEMVWV DVPDCFCFHL WNAWEEAESG EVVVVGSCMT PADSIFNESD
460 470 480 490 500
EHLESVLTEI RLNTRTGEST RRAVLPPAAQ VNLEVGMVNR AMLGRKTRYA
510 520 530 540 550
YLAVAEPWPK VSGFAKVDLA TGELTKFEYG EGRFGGEPCF VPMGGAGAAA
560 570 580 590 600
SPARGEDDGY ILSFVRDEAA GTSELLVVNA ADMRLEATVQ LPSRVPYGFH
610
GTFINAGELA TQA
Sequence cautioni
The sequence BAT18107 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY838901 mRNA Translation: AAW21321.1 DP000011 Genomic DNA Translation: ABA99870.1 AP008218 Genomic DNA Translation: BAH95791.1 AP014968 Genomic DNA Translation: BAT18107.1 Sequence problems. |
RefSeqi | XP_015618707.1, XM_015763221.1 |
Genome annotation databases
GeneIDi | 9270250 |
KEGGi | osa:9270250 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY838901 mRNA Translation: AAW21321.1 DP000011 Genomic DNA Translation: ABA99870.1 AP008218 Genomic DNA Translation: BAH95791.1 AP014968 Genomic DNA Translation: BAT18107.1 Sequence problems. |
RefSeqi | XP_015618707.1, XM_015763221.1 |
3D structure databases
SMRi | Q5MBR3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4530.OS12T0617400-01 |
Proteomic databases
PRIDEi | Q5MBR3 |
Genome annotation databases
GeneIDi | 9270250 |
KEGGi | osa:9270250 |
Phylogenomic databases
eggNOGi | KOG1285, Eukaryota |
InParanoidi | Q5MBR3 |
OrthoDBi | 524712at2759 |
Enzyme and pathway databases
PlantReactomei | R-OSA-1119374, Abscisic acid biosynthesis |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCED5_ORYSJ | |
Accessioni | Q5MBR3Primary (citable) accession number: Q5MBR3 Secondary accession number(s): A0A0P0YC36 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2017 |
Last sequence update: | February 1, 2005 | |
Last modified: | December 2, 2020 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - SIMILARITY comments
Index of protein domains and families