UniProtKB - Q5M868 (GBA2_RAT)
Protein
Non-lysosomal glucosylceramidase
Gene
Gba2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide (By similarity). Glucosylceramides are membrane glycosphingolipids that have a wide intracellular distribution. They are the main precursors of more complex glycosphingolipids that play a role in cellular growth, differentiation, adhesion, signaling, cytoskeletal dynamics and membrane properties. Also involved in the transglucosylation of cholesterol, transferring glucose from glucosylceramides, thereby modifying its water solubility and biological properties. Under specific conditions, may catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (By similarity). Finally, may also play a role in the metabolism of bile acids. It is able to hydrolyze bile acid 3-O-glucosides but also to produce bile acid-glucose conjugates thanks to a bile acid glucosyl transferase activity (By similarity). However, the relevance of both activities is unclear in vivo (By similarity).By similarity
Catalytic activityi
- EC:3.2.1.45By similarityThis reaction proceeds in the forwardBy similarity direction.
- a β-D-glucosyl-(1↔1ʼ)-N-acylsphing-4-enine + cholesterol = an N-acylsphing-4-enine + cholesteryl 3-β-D-glucosideBy similarityThis reaction proceeds in the backwardBy similarity direction.
- chenodeoxycholate + octyl β-D-glucose = β-D-glucosyl-(1→3)-O-chenodeoxycholate + octan-1-olBy similarity
Activity regulationi
Enzymatic activity is dependent on membrane association and requires the presence of lipids.By similarity
: sphingolipid metabolism Pathwayi
This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.
Pathwayi: cholesterol metabolism
This protein is involved in the pathway cholesterol metabolism, which is part of Steroid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway cholesterol metabolism and in Steroid metabolism.
GO - Molecular functioni
- beta-glucosidase activity Source: UniProtKB
- glucosylceramidase activity Source: UniProtKB
- glucosyltransferase activity Source: UniProtKB
- steryl-beta-glucosidase activity Source: UniProtKB
GO - Biological processi
- bile acid metabolic process Source: UniProtKB
- carbohydrate metabolic process Source: InterPro
- central nervous system development Source: GO_Central
- central nervous system neuron development Source: UniProtKB
- cholesterol metabolic process Source: UniProtKB
- glucosylceramide catabolic process Source: UniProtKB
- glycoside catabolic process Source: UniProtKB
- lipid glycosylation Source: UniProtKB
- regulation of actin filament polymerization Source: UniProtKB
- regulation of membrane lipid distribution Source: UniProtKB
- regulation of microtubule polymerization Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Glycosyltransferase, Hydrolase, Transferase |
Biological process | Cholesterol metabolism, Lipid metabolism, Sphingolipid metabolism, Steroid metabolism, Sterol metabolism |
Enzyme and pathway databases
Reactomei | R-RNO-1660662, Glycosphingolipid metabolism |
UniPathwayi | UPA00222 UPA00296 |
Protein family/group databases
CAZyi | GH116, Glycoside Hydrolase Family 116 |
Names & Taxonomyi
Protein namesi | Recommended name: Non-lysosomal glucosylceramidaseCurated (EC:3.2.1.45By similarity)Short name: NLGaseBy similarity Alternative name(s): Beta-glucocerebrosidase 2 Short name: Beta-glucosidase 2 Bile acid beta-glucosidase GBA2By similarity Bile acid glucosyl transferase GBA2By similarity Cholesterol glucosyltransferase GBA2By similarity (EC:2.4.1.-By similarity) Cholesteryl-beta-glucosidase GBA2By similarity (EC:3.2.1.-By similarity) Glucosylceramidase 2 |
Gene namesi | Name:Gba2Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1305598, Gba2 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Localization to the plasma membrane and alternative topologies have also been reported.By similarity
Cytosol
- cytosol Source: UniProtKB
Endoplasmic reticulum
- extrinsic component of endoplasmic reticulum membrane Source: UniProtKB
Golgi apparatus
- extrinsic component of Golgi membrane Source: UniProtKB
Other locations
- extrinsic component of membrane Source: UniProtKB
- integral component of membrane Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000283760 | 1 – 912 | Non-lysosomal glucosylceramidaseAdd BLAST | 912 |
Proteomic databases
PaxDbi | Q5M868 |
PRIDEi | Q5M868 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000016364, Expressed in brain and 22 other tissues |
ExpressionAtlasi | Q5M868, baseline and differential |
Genevisiblei | Q5M868, RN |
Family & Domainsi
Sequence similaritiesi
Belongs to the non-lysosomal glucosylceramidase family.Curated
Phylogenomic databases
eggNOGi | KOG2119, Eukaryota |
GeneTreei | ENSGT00390000010998 |
InParanoidi | Q5M868 |
OMAi | HDLGAPN |
PhylomeDBi | Q5M868 |
TreeFami | TF313888 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR014551, B_Glucosidase_GBA2-typ IPR006775, GH116_catalytic IPR024462, GH116_N |
Pfami | View protein in Pfam PF04685, DUF608, 1 hit PF12215, Glyco_hydr_116N, 1 hit |
PIRSFi | PIRSF028944, Beta_gluc_GBA2, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q5M868-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVTCVPASEQ IGCAERDSQI YSEDTGGTEA VRVTDCRSPE DSGPQNEPGY
60 70 80 90 100
CNSEDSGQLM ASYEGKARGY QVPPFGWRIC LAHEFAEKRK PFQANNVSLS
110 120 130 140 150
NLVKHFGMGL RYLKWWYRKT QVEKKTPFID MFNSVPLRQI YGCPLGGIGG
160 170 180 190 200
GTITRGWRGQ FCRWQLNPGM YQHQTVIADQ FIVCLRRDGR TVYQQVLSLE
210 220 230 240 250
LPSVLRSWNW GLCGYFAFYH ALYPRAWTVY QLPGQNVTLT CRQITPILPH
260 270 280 290 300
DYQDSSLPVG VFVWDVENEG DETLDVSIMF SMRNGLGGED DAAGGLWNEP
310 320 330 340 350
FRLEQDGTTV QGLLLHHPTP PNPYTMAVAA RHTADTTVTY TTAFDPDSTG
360 370 380 390 400
QQVWQDLLQD GQLDSPAGQS TPTQRGEGVA GAVCASSKLL PRGRCCLEFS
410 420 430 440 450
LAWDMPRIMF GAKGQVHYRR YTRFFGSDGD VAPALSHYAL CQYAGWENSI
460 470 480 490 500
SAWQNPVLDD RSLPAWYKSA LFNELYFLAD GGTVWLEVPE DSLPEELGGS
510 520 530 540 550
MYQLRPILQD YGRFGYLEGQ EYRMYNTYDV HFYASFALVM LWPKLELSLQ
560 570 580 590 600
YDMALATFKE DLTRRRYLMS GVVAPVKRRN VIPHDIGDPD DEPWLRVNAY
610 620 630 640 650
LIHDTADWKD LNLKFVLQVY RDYYLTGDQG FLKDMWPVCL AVMESEMKFD
660 670 680 690 700
KDQDGLIENG GYADQTYDGW VTTGPSAYCG GLWLAAVAVM VQMAVLCGAQ
710 720 730 740 750
DVQDKFSSIL CRGREAYERL LWNGRYYNYD SSSQPQSRSV MSDQCAGQWF
760 770 780 790 800
LRACGLGEGD TEVFPTLHVV RALKTIFELN VQAFAGGAMG AVNGMQPHGV
810 820 830 840 850
PDRSSVQSDE VWVGVVYGLA ATMIQEGLTW EGFRTAEGCY RTVWERLGLA
860 870 880 890 900
FQTPEAYCQQ RVFRSLAYMR PLSIWAMQLA LQQQQHKKNS SRPAVTQGTA
910
PSQPECGPKR SL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD4A6U0 | D4A6U0_RAT | Non-lysosomal glucosylceramidase | Gba2 rCG_54897 | 904 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024385 | 181 – 195 | FIVCL…TVYQQ → VRKGAGRRRSDSWLA in isoform 2. 1 PublicationAdd BLAST | 15 | |
Alternative sequenceiVSP_024386 | 196 – 912 | Missing in isoform 2. 1 PublicationAdd BLAST | 717 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AABR03040369 Genomic DNA No translation available. BC088200 mRNA Translation: AAH88200.1 |
Genome annotation databases
Ensembli | ENSRNOT00000022002; ENSRNOP00000022002; ENSRNOG00000016364 [Q5M868-1] |
UCSCi | RGD:1305598, rat [Q5M868-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AABR03040369 Genomic DNA No translation available. BC088200 mRNA Translation: AAH88200.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000022002 |
Protein family/group databases
CAZyi | GH116, Glycoside Hydrolase Family 116 |
Proteomic databases
PaxDbi | Q5M868 |
PRIDEi | Q5M868 |
Genome annotation databases
Ensembli | ENSRNOT00000022002; ENSRNOP00000022002; ENSRNOG00000016364 [Q5M868-1] |
UCSCi | RGD:1305598, rat [Q5M868-1] |
Organism-specific databases
RGDi | 1305598, Gba2 |
Phylogenomic databases
eggNOGi | KOG2119, Eukaryota |
GeneTreei | ENSGT00390000010998 |
InParanoidi | Q5M868 |
OMAi | HDLGAPN |
PhylomeDBi | Q5M868 |
TreeFami | TF313888 |
Enzyme and pathway databases
UniPathwayi | UPA00222 UPA00296 |
Reactomei | R-RNO-1660662, Glycosphingolipid metabolism |
Miscellaneous databases
PROi | PR:Q5M868 |
Gene expression databases
Bgeei | ENSRNOG00000016364, Expressed in brain and 22 other tissues |
ExpressionAtlasi | Q5M868, baseline and differential |
Genevisiblei | Q5M868, RN |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR014551, B_Glucosidase_GBA2-typ IPR006775, GH116_catalytic IPR024462, GH116_N |
Pfami | View protein in Pfam PF04685, DUF608, 1 hit PF12215, Glyco_hydr_116N, 1 hit |
PIRSFi | PIRSF028944, Beta_gluc_GBA2, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GBA2_RAT | |
Accessioni | Q5M868Primary (citable) accession number: Q5M868 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | April 3, 2007 | |
Last modified: | April 7, 2021 | |
This is version 95 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families