Orai1

Functionⁱ

Ca²⁺ release-activated Ca²⁺ (CRAC) channel subunit which mediates Ca²⁺ influx following depletion of intracellular Ca²⁺ stores and channel activation by the Ca²⁺ sensor, STIM1. CRAC channels are the main pathway for Ca²⁺ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.By similarity

GO - Molecular functionⁱ

calmodulin binding Source: UniProtKB-KW
store-operated calcium channel activity
Source: RGD
Inferred from mutant phenotypeⁱ
- 21389210

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adaptive immune response Source: UniProtKB-KW
positive regulation of calcium ion transport Source: UniProtKB
store-operated calcium entry
Source: RGD
Inferred from mutant phenotypeⁱ
- 21389210

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Calcium channel, Calmodulin-binding, Ion channel
Biological process	Adaptive immunity, Calcium transport, Immunity, Ion transport, Transport
Ligand	Calcium

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Calcium release-activated calcium channel protein 1 Alternative name(s): Protein orai-1 Transmembrane protein 142A
Gene namesⁱ	Name:Orai1 Synonyms:Cracm1, Tmem142a
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

Rat genome database More...RGDⁱ	1311873 Orai1

RGDⁱ

1311873 Orai1

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 88	CytoplasmicBy similarityAdd BLAST	88
Transmembraneⁱ	89 – 106	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	107 – 120	ExtracellularSequence analysisAdd BLAST	14
Transmembraneⁱ	121 – 141	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	142 – 174	CytoplasmicSequence analysisAdd BLAST	33
Transmembraneⁱ	175 – 195	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	196 – 237	ExtracellularSequence analysisAdd BLAST	42
Transmembraneⁱ	238 – 258	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	259 – 304	CytoplasmicBy similarityAdd BLAST	46

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL3721308

ChEMBLⁱ

CHEMBL3721308

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000234383	1 – 304	Calcium release-activated calcium channel protein 1Add BLAST		304

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Glycosylationⁱ	225	N-linked (GlcNAc...) asparagineSequence analysis		1
Modified residueⁱ	298	PhosphothreonineBy similarity		1

Post-translational modificationⁱ

N-glycosylated.By similarity

Ubiquitinated.By similarity

Cys-196 is oxidated, leading to inactivate channel activity.By similarity

Keywords - PTMⁱ

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q5M848
PRIDEⁱ	Q5M848

Interactionⁱ

Subunit structureⁱ

Interacts with STIM1 and STIM2; this regulates channel activity (By similarity). Interacts with CALM; this may displace STIM1 and STIM2 and might thereby modulate channel activity (PubMed:23109337). Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca²⁺ (By similarity). Forms a complex with CRACR2A/EFCAB4B and STIM1 at low concentration of Ca²⁺, the complex dissociates at elevated Ca²⁺ concentrations (By similarity). Interacts with SLC35G1 (By similarity). Interacts with UBQLN1 (By similarity).By similarity1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Stim2	D4A5X1	3	EBI-9700451,EBI-9700435

With

Entry

#Exp.

IntAct

Notes

Stim2

D4A5X1

3

EBI-9700451,EBI-9700435

GO - Molecular functionⁱ

calmodulin binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

CORUMⁱ	Q5M848
IntActⁱ	Q5M848, 2 interactors
STRINGⁱ	10116.ENSRNOP00000001806

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	71 – 91	Interaction with STIM1By similarityAdd BLAST		21
Regionⁱ	275 – 295	Interaction with STIM1By similarityAdd BLAST		21

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	43 – 49	Poly-Pro		7

Sequence similaritiesⁱ

Belongs to the Orai family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG4298 Eukaryota ENOG4110DSB LUCA
HOGENOMⁱ	HOG000246925
HOVERGENⁱ	HBG081343
InParanoidⁱ	Q5M848
KEGG Orthology (KO) More...KOⁱ	K16056
PhylomeDBⁱ	Q5M848

Family and domain databases

InterProⁱ	View protein in InterPro IPR012446 CRAC_channel
PANTHERⁱ	PTHR31501 PTHR31501, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07856 Orai-1, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q5M848-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MHPEPAPPPN NSNPELPLSG GSSTSGSRRS RRRSGDGEPT GAPPLPPPPA 
        60         70         80         90        100
VSYPDWIGQS YSEVMSLNEH SMQALSWRKL YLSRAKLKAS SRTSALLSGF 
       110        120        130        140        150
AMVAMVEVQL DTDHDYPPGL LIVFSACTTV LVAVHLFALM ISTCILPNIE 
       160        170        180        190        200
AVSNVHNLNS VKESPHERMH RHIELAWAFS TVIGTLLFLA EVVLLCWVKF 
       210        220        230        240        250
LPLKRQAGQP SPTKPPTEPA VVVANSSNNG GITPGEAAAI ASTAIMVPCG 
       260        270        280        290        300
LVFIVFAVHF YRSLVSHKTD RQFQELNELA EFARLQDQLD HRGDHSLTPG 

THYA

Length:304

Mass (Da):33,040

Last modified:February 1, 2005 - v1

Checksum:ⁱ98264D8880982C74

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	F1LNX2	F1LNX2_RAT		Calcium release-activated calcium c... Calcium release-activated calcium channel protein 1	Orai1	299	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC088225 mRNA Translation: AAH88225.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001014004.1, NM_001013982.1
UniGeneⁱ	Rn.3869

Genome annotation databases

GeneIDⁱ	304496
KEGGⁱ	rno:304496
UCSC genome browser More...UCSCⁱ	RGD:1311873 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q5M848	Calcium release-activated calcium channel protein 1		MOUSE		304	UniRef90_Q8BWG9
Calcium release-activated calcium modulator 1		RAT		304
calcium release-activated calcium channel protein 1		MESAU		302
Calcium release-activated calcium channel protein 1		CRIGR		301
ORAI calcium release-activated calcium modulator 1		HORSE		261
+14

Protein	Similar proteins		Species	Score	Length	Source
Q5M848	Calcium release-activated calcium channel protein 1		MOUSE		304	UniRef50_Q8BWG9
Protein orai-2		XENLA		257
Protein orai-2		HUMAN		254
Calcium release-activated calcium modulator 1		RAT		304
calcium release-activated calcium channel protein 1		MESAU		302
+421

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC088225 mRNA Translation: AAH88225.1
RefSeqⁱ	NP_001014004.1, NM_001013982.1
UniGeneⁱ	Rn.3869

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

CORUMⁱ	Q5M848
IntActⁱ	Q5M848, 2 interactors
STRINGⁱ	10116.ENSRNOP00000001806

Chemistry databases

ChEMBLⁱ	CHEMBL3721308

ChEMBLⁱ

CHEMBL3721308

Proteomic databases

PaxDbⁱ	Q5M848
PRIDEⁱ	Q5M848

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	304496
KEGGⁱ	rno:304496
UCSCⁱ	RGD:1311873 rat

Organism-specific databases

CTDⁱ	84876
RGDⁱ	1311873 Orai1

Phylogenomic databases

eggNOGⁱ	KOG4298 Eukaryota ENOG4110DSB LUCA
HOGENOMⁱ	HOG000246925
HOVERGENⁱ	HBG081343
InParanoidⁱ	Q5M848
KOⁱ	K16056
PhylomeDBⁱ	Q5M848

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q5M848

PROⁱ

PR:Q5M848

Family and domain databases

InterProⁱ	View protein in InterPro IPR012446 CRAC_channel
PANTHERⁱ	PTHR31501 PTHR31501, 1 hit
Pfamⁱ	View protein in Pfam PF07856 Orai-1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CRCM1_RAT
Accessionⁱ	Q5M848Primary (citable) accession number: Q5M848
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
	Last sequence update:	February 1, 2005
	Last modified:	November 7, 2018
	This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

UniProtKB

UniParc

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UniProtKB - Q5M848 (CRCM1_RAT)

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Calcium release-activated calcium channel protein 1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

GO - Molecular functionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ