UniProtKB - Q5M848 (CRCM1_RAT)
Calcium release-activated calcium channel protein 1
Orai1
Functioni
Caution
Activity regulationi
GO - Molecular functioni
- calcium channel activity Source: RGD
- calmodulin binding Source: UniProtKB-KW
- identical protein binding Source: RGD
- store-operated calcium channel activity Source: RGD
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- calcium ion import Source: RGD
- calcium ion transmembrane transport Source: RGD
- mammary gland epithelium development Source: RGD
- positive regulation of adenylate cyclase activity Source: UniProtKB
- positive regulation of calcium ion transport Source: UniProtKB
- regulation of calcium ion transport Source: RGD
- store-operated calcium entry Source: RGD
Keywordsi
Molecular function | Calcium channel, Calmodulin-binding, Ion channel |
Biological process | Adaptive immunity, Calcium transport, Immunity, Ion transport, Transport |
Ligand | Calcium |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium release-activated calcium channel protein 1Alternative name(s): Protein orai-1 Transmembrane protein 142A |
Gene namesi | Name:Orai1 Synonyms:Cracm1, Tmem142a |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1311873, Orai1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Colocalizes with STIM1 at the cell membrane.By similarity
Plasma Membrane
- basolateral plasma membrane Source: RGD
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: RGD
- plasma membrane raft Source: UniProtKB
Other locations
- growth cone Source: RGD
- membrane Source: RGD
- membrane raft Source: UniProtKB
- protein-containing complex Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 88 | CytoplasmicBy similarityAdd BLAST | 88 | |
Transmembranei | 89 – 106 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 107 – 120 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 121 – 141 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 142 – 174 | CytoplasmicSequence analysisAdd BLAST | 33 | |
Transmembranei | 175 – 195 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 196 – 237 | ExtracellularSequence analysisAdd BLAST | 42 | |
Transmembranei | 238 – 258 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 259 – 304 | CytoplasmicBy similarityAdd BLAST | 46 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000234383 | 1 – 304 | Calcium release-activated calcium channel protein 1Add BLAST | 304 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 225 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 298 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q5M848 |
PRIDEi | Q5M848 |
PTM databases
GlyGeni | Q5M848, 1 site |
Interactioni
Subunit structurei
Interacts with STIM1 and STIM2; this regulates channel activity (By similarity).
Interacts with CALM; this may displace STIM1 and STIM2 and might thereby modulate channel activity (PubMed:23109337).
Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca2+ (By similarity).
Forms a complex with CRACR2A/EFCAB4B and STIM1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations (By similarity).
Interacts with SLC35G1 (By similarity).
Interacts with UBQLN1 (By similarity).
Interacts with ADCY8; interaction is calcium store depletion independent; interaction occurs in membrane raft; interaction increases markedly after store depletion; positively regulates SOCE-induced adenylate cyclase activity; contributes to the targeting of ADCY8 to discrete regions of the plasma membrane that are shielded from other calcium events (By similarity).
Interacts with EFHB; the interaction takes place upon Ca2+-store depletion (By similarity).
By similarity1 PublicationBinary interactionsi
Q5M848
With | #Exp. | IntAct |
---|---|---|
Stim2 [D4A5X1] | 3 | EBI-9700451,EBI-9700435 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- identical protein binding Source: RGD
Protein-protein interaction databases
CORUMi | Q5M848 |
IntActi | Q5M848, 2 interactors |
STRINGi | 10116.ENSRNOP00000001806 |
Structurei
3D structure databases
BMRBi | Q5M848 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 71 – 91 | Interaction with STIM1By similarityAdd BLAST | 21 | |
Regioni | 275 – 295 | Interaction with STIM1By similarityAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 43 – 49 | Poly-Pro | 7 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4298, Eukaryota |
InParanoidi | Q5M848 |
OrthoDBi | 1197243at2759 |
PhylomeDBi | Q5M848 |
Family and domain databases
InterProi | View protein in InterPro IPR012446, CRAC_channel |
PANTHERi | PTHR31501, PTHR31501, 1 hit |
Pfami | View protein in Pfam PF07856, Orai-1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MHPEPAPPPN NSNPELPLSG GSSTSGSRRS RRRSGDGEPT GAPPLPPPPA
60 70 80 90 100
VSYPDWIGQS YSEVMSLNEH SMQALSWRKL YLSRAKLKAS SRTSALLSGF
110 120 130 140 150
AMVAMVEVQL DTDHDYPPGL LIVFSACTTV LVAVHLFALM ISTCILPNIE
160 170 180 190 200
AVSNVHNLNS VKESPHERMH RHIELAWAFS TVIGTLLFLA EVVLLCWVKF
210 220 230 240 250
LPLKRQAGQP SPTKPPTEPA VVVANSSNNG GITPGEAAAI ASTAIMVPCG
260 270 280 290 300
LVFIVFAVHF YRSLVSHKTD RQFQELNELA EFARLQDQLD HRGDHSLTPG
THYA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1LNX2 | F1LNX2_RAT | Calcium release-activated calcium c... | Orai1 | 299 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC088225 mRNA Translation: AAH88225.1 |
RefSeqi | NP_001014004.1, NM_001013982.1 |
Genome annotation databases
GeneIDi | 304496 |
KEGGi | rno:304496 |
UCSCi | RGD:1311873, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC088225 mRNA Translation: AAH88225.1 |
RefSeqi | NP_001014004.1, NM_001013982.1 |
3D structure databases
BMRBi | Q5M848 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
CORUMi | Q5M848 |
IntActi | Q5M848, 2 interactors |
STRINGi | 10116.ENSRNOP00000001806 |
Chemistry databases
ChEMBLi | CHEMBL3721308 |
PTM databases
GlyGeni | Q5M848, 1 site |
Proteomic databases
PaxDbi | Q5M848 |
PRIDEi | Q5M848 |
Genome annotation databases
GeneIDi | 304496 |
KEGGi | rno:304496 |
UCSCi | RGD:1311873, rat |
Organism-specific databases
CTDi | 84876 |
RGDi | 1311873, Orai1 |
Phylogenomic databases
eggNOGi | KOG4298, Eukaryota |
InParanoidi | Q5M848 |
OrthoDBi | 1197243at2759 |
PhylomeDBi | Q5M848 |
Miscellaneous databases
PROi | PR:Q5M848 |
Family and domain databases
InterProi | View protein in InterPro IPR012446, CRAC_channel |
PANTHERi | PTHR31501, PTHR31501, 1 hit |
Pfami | View protein in Pfam PF07856, Orai-1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CRCM1_RAT | |
Accessioni | Q5M848Primary (citable) accession number: Q5M848 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | February 1, 2005 | |
Last modified: | December 2, 2020 | |
This is version 97 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families