UniProtKB - Q5M7W5 (MAP4_RAT)
Protein
Microtubule-associated protein 4
Gene
Map4
Organism
Rattus norvegicus (Rat)
Status
Functioni
Non-neuronal microtubule-associated protein. Promotes microtubule assembly (By similarity).By similarity
GO - Molecular functioni
- microtubule binding Source: RGD
GO - Biological processi
- cell division Source: RGD
- establishment of spindle orientation Source: RGD
- microtubule cytoskeleton organization Source: RGD
- microtubule sliding Source: RGD
- mitotic spindle organization Source: RGD
- negative regulation of non-motile cilium assembly Source: RGD
- neuron projection development Source: GO_Central
Names & Taxonomyi
Protein namesi | Recommended name: Microtubule-associated protein 4Short name: MAP-4 |
Gene namesi | Name:Map4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1564359, Map4 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Cytoskeleton
- axoneme Source: RGD
- microtubule Source: UniProtKB-KW
- mitotic spindle Source: RGD
Other locations
- axon Source: ARUK-UCL
- neuron projection Source: GO_Central
- postsynaptic density Source: RGD
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000323721 | 2 – 1057 | Microtubule-associated protein 4Add BLAST | 1056 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 5 | PhosphoserineBy similarity | 1 | |
Modified residuei | 60 | PhosphoserineBy similarity | 1 | |
Modified residuei | 99 | PhosphoserineBy similarity | 1 | |
Modified residuei | 254 | PhosphoserineBy similarity | 1 | |
Modified residuei | 257 | PhosphoserineCombined sources | 1 | |
Modified residuei | 417 | PhosphoserineBy similarity | 1 | |
Modified residuei | 419 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 450 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 497 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 506 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 509 | PhosphoserineCombined sources | 1 | |
Modified residuei | 515 | PhosphoserineCombined sources | 1 | |
Modified residuei | 520 | PhosphoserineCombined sources | 1 | |
Modified residuei | 522 | PhosphoserineCombined sources | 1 | |
Modified residuei | 546 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 599 | PhosphoserineBy similarity | 1 | |
Modified residuei | 618 | PhosphoserineCombined sources | 1 | |
Modified residuei | 659 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 668 | PhosphoserineBy similarity | 1 | |
Modified residuei | 685 | PhosphoserineBy similarity | 1 | |
Modified residuei | 695 | PhosphoserineBy similarity | 1 | |
Modified residuei | 761 | PhosphoserineBy similarity | 1 | |
Modified residuei | 799 | PhosphoserineBy similarity | 1 | |
Modified residuei | 801 | PhosphoserineBy similarity | 1 | |
Cross-linki | 812 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 827 | PhosphoserineBy similarity | 1 | |
Modified residuei | 902 | PhosphoserineCombined sources | 1 | |
Modified residuei | 915 | PhosphoserineCombined sources | 1 | |
Modified residuei | 915 | Phosphoserine; by MARK1Combined sources1 Publication | 1 | |
Modified residuei | 916 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 978 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1050 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1056 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylation on Ser-761 negatively regulates MAP4 activity to promote microtubule assembly. Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly.1 Publication
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q5M7W5 |
PRIDEi | Q5M7W5 |
PTM databases
iPTMneti | Q5M7W5 |
PhosphoSitePlusi | Q5M7W5 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000020748, Expressed in skeletal muscle tissue and 21 other tissues |
ExpressionAtlasi | Q5M7W5, baseline and differential |
Genevisiblei | Q5M7W5, RN |
Interactioni
Subunit structurei
Interacts with SEPTIN2; this interaction impedes tubulin-binding (By similarity).
Interacts with TRAF3IP1 (By similarity).
By similarityGO - Molecular functioni
- microtubule binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 266360, 3 interactors |
IntActi | Q5M7W5, 2 interactors |
STRINGi | 10116.ENSRNOP00000053011 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 897 – 927 | Tau/MAP 1Add BLAST | 31 | |
Repeati | 928 – 958 | Tau/MAP 2Add BLAST | 31 | |
Repeati | 959 – 990 | Tau/MAP 3Add BLAST | 32 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2418, Eukaryota |
GeneTreei | ENSGT00940000164123 |
HOGENOMi | CLU_012370_0_0_1 |
InParanoidi | Q5M7W5 |
OMAi | KQHEHRP |
OrthoDBi | 716848at2759 |
PhylomeDBi | Q5M7W5 |
Family and domain databases
InterProi | View protein in InterPro IPR027323, MAP4 IPR001084, MAP_tubulin-bd_rpt |
PANTHERi | PTHR11501:SF16, PTHR11501:SF16, 2 hits |
Pfami | View protein in Pfam PF00418, Tubulin-binding, 3 hits |
PROSITEi | View protein in PROSITE PS00229, TAU_MAP_1, 3 hits PS51491, TAU_MAP_2, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q5M7W5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADLSLVDAL TEPPPEIEGE IKRDFMAALE AEPYDDIVGE TVEKTEFIPL
60 70 80 90 100
LDGDEKSGNS ESKRKPCVDT SQVEDIPSSK PTLLANGDHG VEGNNTTGSP
110 120 130 140 150
TDFLVENVDY EDYQNSQSWP EDASFCFQPQ QVLDTNQADP FNVHHDDGLA
160 170 180 190 200
DLLFVSSGPT NASAFIEENN PLEDSYGVLP CDSFAPTAVV SQEWSVGAPD
210 220 230 240 250
SPHSEPCVSP EVTIETAQPA TELSKAVDLE SVKEQLPAKA LEMMAGQTTD
260 270 280 290 300
AVPSKESEGS PDTDAAPGPD TDVTLTKDIE ESSSPDVISA NVTQPFTESD
310 320 330 340 350
MFLTQEMELL IGTEAAQVKD TMSSVEPDIS SAKNTAPPTE EETVPGKDMT
360 370 380 390 400
FPKEAETALP IEMDLAPPED VALPKETELE LAPAAGTAPL SETEVALAKD
410 420 430 440 450
EEPSTGIPAA QEMLLSSETE VVLPSDSIMT LTKEVTVPLE AAGPLVSDMT
460 470 480 490 500
AILETEMTLG GGTATPTETK LGKVKDMAPL PESEVALGKD VVTLPETKVT
510 520 530 540 550
EFNNVTPLSE EEVASIKDVS PSPETETAKN ADLHSGTELT LDNSMTPPSD
560 570 580 590 600
PALPLETKVA TVQIKDKETV QTQEELSEDS QLESVQLEGQ SAVPPCTISP
610 620 630 640 650
EPVKAADQKS TLPVDEGSPL EKLEQKETSG SQPPELCSGV SRQEEGKAAV
660 670 680 690 700
GLTGNDIATP PNKELPPSPE KKAKPLATTQ PAKTSTSKAK IQPTSLPKQP
710 720 730 740 750
APTTSGGLNK KPMSLASGSV PAAPHKRPAA ATATARPSTL PARDLKPKPI
760 770 780 790 800
TETKVAEKRT SPSKPSSAPA LRPGPKTTPT ISKATSPSTL VSTGSSSRSP
810 820 830 840 850
STTLPKRPTT TKTEGKPADV KRMTAKSATA DLSRSKTTSA SSVKRNTTPT
860 870 880 890 900
GATPPAGMAS TRVKPMSAPC RSSVALSVDK KPTSTKPSSS APRVSRLATT
910 920 930 940 950
VSAPDLKSVR SKVGSTENMK HQPGGGRVQI QNKKVDISKV SSKCGSKANI
960 970 980 990 1000
KHKPGGGDVK IESQKLNFKE KAQAKVGSLD NVGHLPAGGT VKTEGGGSEA
1010 1020 1030 1040 1050
PPCPGPPAGE EPAIPEAAPD AGAPTSASGL SGHTTLSGGG DQREPQTLDS
QIQETSI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JW88 | A0A0G2JW88_RAT | Microtubule-associated protein | Map4 | 2,201 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032080 | 674 – 747 | Missing in isoform 2. 1 PublicationAdd BLAST | 74 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC088405 mRNA Translation: AAH88405.1 AB175782 mRNA Translation: BAD14292.1 AB175783 mRNA Translation: BAD14293.1 |
RefSeqi | NP_001019449.1, NM_001024278.1 [Q5M7W5-1] |
Genome annotation databases
Ensembli | ENSRNOT00000056166; ENSRNOP00000053011; ENSRNOG00000020748 [Q5M7W5-1] |
GeneIDi | 367171 |
KEGGi | rno:367171 |
UCSCi | RGD:1564359, rat [Q5M7W5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC088405 mRNA Translation: AAH88405.1 AB175782 mRNA Translation: BAD14292.1 AB175783 mRNA Translation: BAD14293.1 |
RefSeqi | NP_001019449.1, NM_001024278.1 [Q5M7W5-1] |
3D structure databases
SMRi | Q5M7W5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 266360, 3 interactors |
IntActi | Q5M7W5, 2 interactors |
STRINGi | 10116.ENSRNOP00000053011 |
PTM databases
iPTMneti | Q5M7W5 |
PhosphoSitePlusi | Q5M7W5 |
Proteomic databases
jPOSTi | Q5M7W5 |
PRIDEi | Q5M7W5 |
Genome annotation databases
Ensembli | ENSRNOT00000056166; ENSRNOP00000053011; ENSRNOG00000020748 [Q5M7W5-1] |
GeneIDi | 367171 |
KEGGi | rno:367171 |
UCSCi | RGD:1564359, rat [Q5M7W5-1] |
Organism-specific databases
CTDi | 4134 |
RGDi | 1564359, Map4 |
Phylogenomic databases
eggNOGi | KOG2418, Eukaryota |
GeneTreei | ENSGT00940000164123 |
HOGENOMi | CLU_012370_0_0_1 |
InParanoidi | Q5M7W5 |
OMAi | KQHEHRP |
OrthoDBi | 716848at2759 |
PhylomeDBi | Q5M7W5 |
Miscellaneous databases
PROi | PR:Q5M7W5 |
Gene expression databases
Bgeei | ENSRNOG00000020748, Expressed in skeletal muscle tissue and 21 other tissues |
ExpressionAtlasi | Q5M7W5, baseline and differential |
Genevisiblei | Q5M7W5, RN |
Family and domain databases
InterProi | View protein in InterPro IPR027323, MAP4 IPR001084, MAP_tubulin-bd_rpt |
PANTHERi | PTHR11501:SF16, PTHR11501:SF16, 2 hits |
Pfami | View protein in Pfam PF00418, Tubulin-binding, 3 hits |
PROSITEi | View protein in PROSITE PS00229, TAU_MAP_1, 3 hits PS51491, TAU_MAP_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAP4_RAT | |
Accessioni | Q5M7W5Primary (citable) accession number: Q5M7W5 Secondary accession number(s): Q75NR5, Q75NR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | February 1, 2005 | |
Last modified: | April 7, 2021 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |