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Protein

4-O-beta-D-mannosyl-D-glucose phosphorylase

Gene

BF0772

Organism
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc) to mannose 1-phosphate (Man1P) and glucose. Involved in a mannan catabolic pathway which feeds into glycolysis.UniRule annotation1 Publication

Catalytic activityi

4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + alpha-D-mannose 1-phosphate.UniRule annotation1 Publication

Kineticsi

  1. KM=8.3 mM for Man-Glc1 Publication
  2. KM=2.0 mM for phosphate1 Publication

    pH dependencei

    Optimum pH is 7.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processCarbohydrate metabolism, Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18532
    BRENDAi2.4.1.281 755

    Protein family/group databases

    CAZyiGH130 Glycoside Hydrolase Family 130

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-O-beta-D-mannosyl-D-glucose phosphorylaseUniRule annotation (EC:2.4.1.281UniRule annotation)
    Short name:
    MGPUniRule annotation
    Short name:
    Mannosylglucose phosphorylaseUniRule annotation
    Gene namesi
    Ordered Locus Names:BF0772
    ORF Names:BF9343_0737
    OrganismiBacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
    Taxonomic identifieri272559 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    Proteomesi
    • UP000006731 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004188601 – 3904-O-beta-D-mannosyl-D-glucose phosphorylaseAdd BLAST390

    Proteomic databases

    PRIDEiQ5LH68

    Interactioni

    Protein-protein interaction databases

    STRINGi272559.BF0772

    Structurei

    Secondary structure

    1390
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 20Combined sources18
    Beta strandi34 – 38Combined sources5
    Helixi43 – 45Combined sources3
    Helixi48 – 51Combined sources4
    Turni56 – 58Combined sources3
    Beta strandi64 – 66Combined sources3
    Beta strandi68 – 79Combined sources12
    Beta strandi82 – 91Combined sources10
    Beta strandi96 – 108Combined sources13
    Beta strandi127 – 136Combined sources10
    Beta strandi142 – 151Combined sources10
    Beta strandi153 – 155Combined sources3
    Beta strandi163 – 177Combined sources15
    Beta strandi179 – 181Combined sources3
    Beta strandi187 – 190Combined sources4
    Beta strandi203 – 208Combined sources6
    Beta strandi210 – 215Combined sources6
    Beta strandi222 – 229Combined sources8
    Helixi231 – 233Combined sources3
    Beta strandi240 – 244Combined sources5
    Beta strandi252 – 257Combined sources6
    Beta strandi262 – 264Combined sources3
    Beta strandi267 – 278Combined sources12
    Beta strandi281 – 291Combined sources11
    Beta strandi298 – 301Combined sources4
    Beta strandi303 – 305Combined sources3
    Helixi312 – 315Combined sources4
    Beta strandi321 – 330Combined sources10
    Beta strandi334 – 342Combined sources9
    Turni343 – 345Combined sources3
    Beta strandi346 – 353Combined sources8
    Helixi354 – 363Combined sources10
    Helixi371 – 389Combined sources19

    3D structure databases

    ProteinModelPortaliQ5LH68
    SMRiQ5LH68
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 130 family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105HY2 Bacteria
    COG2152 LUCA
    HOGENOMiHOG000252436
    KOiK16212
    OMAiYAFYTRP

    Family and domain databases

    Gene3Di2.115.10.20, 1 hit
    HAMAPiMF_00928 Man_Glc_phosphorylase, 1 hit
    InterProiView protein in InterPro
    IPR023296 Glyco_hydro_beta-prop_sf
    IPR028583 Man_Glc_phosphorylase
    IPR007184 Mannoside_phosphorylase
    PANTHERiPTHR34106 PTHR34106, 1 hit
    PfamiView protein in Pfam
    PF04041 Glyco_hydro_130, 1 hit
    PIRSFiPIRSF016202 PH1107, 1 hit
    SUPFAMiSSF75005 SSF75005, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q5LH68-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSLFNDKVAK LLAGHEALLM RKNEPVEEGN GVITRYRYPV LTAAHTPVFW
    60 70 80 90 100
    RYDLNEETNP FLMERIGMNA TLNAGAIKWD GKYLMLVRVE GADRKSFFAV
    110 120 130 140 150
    AESPNGIDNF RFWEYPVTLP EDVVPATNVY DMRLTAHEDG WIYGIFCAER
    160 170 180 190 200
    HDDNAPIGDL SSATATAGIA RTKDLKNWER LPDLKTKSQQ RNVVLHPEFV
    210 220 230 240 250
    DGKYALYTRP QDGFIDTGSG GGIGWALIDD ITHAEVGEEK IIDKRYYHTI
    260 270 280 290 300
    KEVKNGEGPH PIKTPQGWLH LAHGVRNCAA GLRYVLYMYM TSLDDPTRLI
    310 320 330 340 350
    ASPAGYFMAP VGEERIGDVS NVLFSNGWIA DDDGKVFIYY ASSDTRMHVA
    360 370 380 390
    TSTIERLVDY CLHTPQDGFS SSASVEILKN LIERNLRLMK
    Length:390
    Mass (Da):43,886
    Last modified:June 21, 2005 - v1
    Checksum:i9C55CBE41B7AFEC1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CR626927 Genomic DNA Translation: CAH06518.1
    RefSeqiWP_005785018.1, NC_003228.3

    Genome annotation databases

    EnsemblBacteriaiCAH06518; CAH06518; BF9343_0737
    KEGGibfs:BF9343_0737

    Similar proteinsi

    Entry informationi

    Entry nameiMGP_BACFN
    AccessioniPrimary (citable) accession number: Q5LH68
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: June 21, 2005
    Last modified: May 23, 2018
    This is version 61 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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