UniProtKB - Q5L0Y0 (MURE_GEOKA)
Protein
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Gene
murE
Organism
Geobacillus kaustophilus (strain HTA426)
Status
Functioni
Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation
Catalytic activityi
- ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate = ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminoheptanedioateUniRule annotationEC:6.3.2.13UniRule annotation
Cofactori
Mg2+UniRule annotation
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 30 | UDP-MurNAc-L-Ala-D-GluUniRule annotation | 1 | |
Binding sitei | 149 | UDP-MurNAc-L-Ala-D-GluUniRule annotation | 1 | |
Binding sitei | 177 | UDP-MurNAc-L-Ala-D-GluUniRule annotation | 1 | |
Binding sitei | 183 | UDP-MurNAc-L-Ala-D-GluUniRule annotation | 1 | |
Binding sitei | 185 | UDP-MurNAc-L-Ala-D-GluUniRule annotation | 1 | |
Binding sitei | 383 | Meso-diaminopimelateUniRule annotation | 1 | |
Binding sitei | 459 | Meso-diaminopimelate; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 463 | Meso-diaminopimelateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 108 – 114 | ATPUniRule annotation | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniRule
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)Alternative name(s): Meso-A2pm-adding enzymeUniRule annotation Meso-diaminopimelate-adding enzymeUniRule annotation UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation UDP-MurNAc-tripeptide synthetaseUniRule annotation UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation |
Gene namesi | Name:murEUniRule annotation Ordered Locus Names:GK1115 |
Organismi | Geobacillus kaustophilus (strain HTA426) |
Taxonomic identifieri | 235909 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › Geobacillus thermoleovorans group › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000012352 | 1 – 489 | UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseAdd BLAST | 489 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 217 | N6-carboxylysineUniRule annotation | 1 |
Post-translational modificationi
Carboxylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 150 – 151 | UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation | 2 | |
Regioni | 407 – 410 | Meso-diaminopimelate bindingUniRule annotation | 4 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 407 – 410 | Meso-diaminopimelate recognition motif | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0769, Bacteria |
HOGENOMi | CLU_022291_4_1_9 |
OMAi | CFMEVSS |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_00208, MurE, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005761, UDP-N-AcMur-Glu-dNH2Pim_ligase |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01085, murE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5L0Y0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLLTLLSHL PGFWVHHGGN PDIVALEMDS RRVASGSLFF CLKGFTVDGH
60 70 80 90 100
DFAEEAVARG AAAIVAERPL SVDVPVVLVS DSRRAMAILA DAFYGRPTHR
110 120 130 140 150
LHLIGVTGTN GKTTTTSIIE QIARKTGKKT GLIGTVHIKV GDRSYPAANT
160 170 180 190 200
TPESLILQRT FKQMVDEGVE FVAMEVSSHA LHQGRVHGCD YDVAVFTNLT
210 220 230 240 250
QDHLDYHGTM EEYRNAKGLL FAQLGNRYDE RRPKFAVLNH DDPVSQYYKH
260 270 280 290 300
MTAAPIVTYG MREKSDVMAE QIRMTAGGMA FRLCTPHGSA AVETKLVGSF
310 320 330 340 350
NVYNILAAAA ACLASGFSLE TIAAALADVE PVPGRFETVD EGQNFTIIVD
360 370 380 390 400
YAHTPDSLEN ALKTVRQLAK RNVYVVIGCG GDRDPSKRPL MAQVAVRYAD
410 420 430 440 450
VAIFTSDNPR SEDPKQILRD MEAGVSAEIG KHVTIPDREE AIRYAIGQAQ
460 470 480
EGDVVLIAGK GHETYQIIGN DVIEFDDRAV ARAAVKERG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000043 Genomic DNA Translation: BAD75400.1 |
RefSeqi | WP_011230615.1, NC_006510.1 |
Genome annotation databases
EnsemblBacteriai | BAD75400; BAD75400; GK1115 |
KEGGi | gka:GK1115 |
PATRICi | fig|235909.7.peg.1215 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000043 Genomic DNA Translation: BAD75400.1 |
RefSeqi | WP_011230615.1, NC_006510.1 |
3D structure databases
SMRi | Q5L0Y0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 235909.GK1115 |
Genome annotation databases
EnsemblBacteriai | BAD75400; BAD75400; GK1115 |
KEGGi | gka:GK1115 |
PATRICi | fig|235909.7.peg.1215 |
Phylogenomic databases
eggNOGi | COG0769, Bacteria |
HOGENOMi | CLU_022291_4_1_9 |
OMAi | CFMEVSS |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_00208, MurE, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005761, UDP-N-AcMur-Glu-dNH2Pim_ligase |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01085, murE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MURE_GEOKA | |
Accessioni | Q5L0Y0Primary (citable) accession number: Q5L0Y0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | February 1, 2005 | |
Last modified: | December 2, 2020 | |
This is version 102 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families