UniProtKB - Q5KSL6 (DGKK_HUMAN)
Protein
Diacylglycerol kinase kappa
Gene
DGKK
Organism
Homo sapiens (Human)
Status
Functioni
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:16210324, PubMed:23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable).Curated2 Publications
Catalytic activityi
- EC:2.7.1.1072 Publications
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+2 PublicationsThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Inhibited in response to H2O2.1 Publication
: glycerolipid metabolism Pathwayi
This protein is involved in the pathway glycerolipid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway glycerolipid metabolism and in Lipid metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 327 – 377 | Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST | 51 | |
Zinc fingeri | 398 – 449 | Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST | 52 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- diacylglycerol kinase activity Source: HGNC-UCL
- metal ion binding Source: UniProtKB-KW
- NAD+ kinase activity Source: InterPro
GO - Biological processi
- diacylglycerol metabolic process Source: HGNC-UCL
- intracellular signal transduction Source: HGNC-UCL
- lipid phosphorylation Source: GO_Central
- platelet activation Source: Reactome
- protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
- response to oxidative stress Source: HGNC-UCL
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q5KSL6 |
Reactomei | R-HSA-114508, Effects of PIP2 hydrolysis |
UniPathwayi | UPA00230 |
Chemistry databases
SwissLipidsi | SLP:000000926 |
Names & Taxonomyi
Protein namesi | Recommended name: Diacylglycerol kinase kappa1 Publication (EC:2.7.1.1072 Publications)Short name: DAG kinase kappaCurated Short name: DGK-kappa1 Publication Alternative name(s): 142 kDa diacylglycerol kinase1 Publication Diglyceride kinase kappa |
Gene namesi | Name:DGKKImported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:32395, DGKK |
MIMi | 300837, gene |
neXtProti | NX_Q5KSL6 |
VEuPathDBi | HostDB:ENSG00000274588.1 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: HGNC-UCL
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 78 | Y → F: Induces a strong reduction in phosphorylation but is still sensitive to H(2)O(2). 1 Publication | 1 | |
Mutagenesisi | 1075 | Y → F: Does not affect phosphorylation. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 139189 |
OpenTargetsi | ENSG00000274588 |
PharmGKBi | PA142671978 |
Miscellaneous databases
Pharosi | Q5KSL6, Tbio |
Chemistry databases
DrugBanki | DB14001, alpha-Tocopherol succinate |
Genetic variation databases
BioMutai | DGKK |
DMDMi | 74708075 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000239368 | 1 – 1271 | Diacylglycerol kinase kappaAdd BLAST | 1271 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 78 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Phosphorylated at Tyr-78 by some member of the SRC family in response to H2O2.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q5KSL6 |
MassIVEi | Q5KSL6 |
MaxQBi | Q5KSL6 |
PeptideAtlasi | Q5KSL6 |
PRIDEi | Q5KSL6 |
ProteomicsDBi | 63549 |
PTM databases
iPTMneti | Q5KSL6 |
PhosphoSitePlusi | Q5KSL6 |
Expressioni
Tissue specificityi
Expressed in testis, and to a lesser extent in placenta.1 Publication
Gene expression databases
Bgeei | ENSG00000274588, Expressed in metanephros and 55 other tissues |
Genevisiblei | Q5KSL6, HS |
Organism-specific databases
HPAi | ENSG00000274588, Group enriched (adrenal gland, brain, pituitary gland) |
Interactioni
Subunit structurei
Does not form homooligomers.
1 PublicationProtein-protein interaction databases
BioGRIDi | 126548, 1 interactor |
IntActi | Q5KSL6, 1 interactor |
STRINGi | 9606.ENSP00000477515 |
Miscellaneous databases
RNActi | Q5KSL6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 48 – 51 | 1 | 4 | |
Repeati | 52 – 55 | 2 | 4 | |
Repeati | 56 – 59 | 3 | 4 | |
Repeati | 60 – 63 | 4 | 4 | |
Repeati | 64 – 67 | 5 | 4 | |
Repeati | 68 – 71 | 6 | 4 | |
Repeati | 72 – 75 | 7 | 4 | |
Repeati | 76 – 79 | 8 | 4 | |
Repeati | 80 – 83 | 9 | 4 | |
Repeati | 84 – 87 | 10 | 4 | |
Repeati | 88 – 91 | 11 | 4 | |
Repeati | 92 – 95 | 12 | 4 | |
Repeati | 96 – 99 | 13 | 4 | |
Repeati | 100 – 103 | 14 | 4 | |
Repeati | 104 – 107 | 15 | 4 | |
Repeati | 108 – 111 | 16 | 4 | |
Repeati | 112 – 115 | 17 | 4 | |
Repeati | 116 – 119 | 18 | 4 | |
Repeati | 120 – 123 | 19 | 4 | |
Repeati | 124 – 127 | 20 | 4 | |
Repeati | 128 – 131 | 21 | 4 | |
Repeati | 132 – 135 | 22 | 4 | |
Repeati | 136 – 139 | 23 | 4 | |
Repeati | 140 – 143 | 24 | 4 | |
Repeati | 144 – 147 | 25 | 4 | |
Repeati | 148 – 151 | 26 | 4 | |
Repeati | 152 – 155 | 27 | 4 | |
Repeati | 156 – 159 | 28 | 4 | |
Repeati | 160 – 163 | 29 | 4 | |
Repeati | 164 – 167 | 30 | 4 | |
Repeati | 168 – 171 | 31 | 4 | |
Repeati | 172 – 175 | 32 | 4 | |
Repeati | 176 – 179 | 33 | 4 | |
Domaini | 216 – 309 | PHPROSITE-ProRule annotationAdd BLAST | 94 | |
Domaini | 487 – 622 | DAGKcPROSITE-ProRule annotationAdd BLAST | 136 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 48 – 179 | 33 X 4 AA approximate tandem repeats of E-P-A-PAdd BLAST | 132 | |
Regioni | 1199 – 1268 | Required for localization to the plasma membrane1 PublicationAdd BLAST | 70 |
Sequence similaritiesi
Belongs to the eukaryotic diacylglycerol kinase family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 327 – 377 | Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST | 51 | |
Zinc fingeri | 398 – 449 | Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST | 52 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1170, Eukaryota |
GeneTreei | ENSGT00940000162262 |
HOGENOMi | CLU_001799_3_3_1 |
InParanoidi | Q5KSL6 |
OMAi | ECKHTEI |
OrthoDBi | 43384at2759 |
PhylomeDBi | Q5KSL6 |
Family and domain databases
CDDi | cd00029, C1, 2 hits |
Gene3Di | 2.30.29.30, 1 hit 3.40.50.10330, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR037607, DGK IPR000756, Diacylglycerol_kin_accessory IPR001206, Diacylglycerol_kinase_cat_dom IPR016064, NAD/diacylglycerol_kinase_sf IPR002219, PE/DAG-bd IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR11255, PTHR11255, 1 hit |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF00609, DAGK_acc, 1 hit PF00781, DAGK_cat, 1 hit PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 2 hits SM00045, DAGKa, 1 hit SM00046, DAGKc, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
PROSITEi | View protein in PROSITE PS50146, DAGK, 1 hit PS50003, PH_DOMAIN, 1 hit PS00479, ZF_DAG_PE_1, 2 hits PS50081, ZF_DAG_PE_2, 2 hits |
i Sequence
Sequence statusi: Complete.
Q5KSL6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDRGAAAAQG TAPPQDGEQP AESPEPPPPW PPPPPPPAPP PAPPLLSEAS
60 70 80 90 100
PEPIPEPCPE LAPGPCPEAT SESATELYTE PTPEPATEPA SEPAPEPATE
110 120 130 140 150
PAPEPATEPA PEPAPEPATE SAPEPTPEPA LESVPEPAPE LTPEVAPELA
160 170 180 190 200
PEPTPEPVTE LAPEFCPEAA PEFRPSPAPC LLQCPVDTRE RGLKTSPSPS
210 220 230 240 250
PSPSPRTPMS WSRIKKILKE GPMLKNCNSF KRWKLRYFLV QGQKLYFAHH
260 270 280 290 300
PAFAHFETID LSQATVAESS CRNLCHSFCV ITPQRKITLA APNRKDMEEW
310 320 330 340 350
INIIKTIQQG EIYKIPAAEN NPFLVGMHCW YSSYSHRTQH CNVCRESIPA
360 370 380 390 400
LSRDAIICEV CKVKSHRLCA LRASKDCKWN TLSITDDLLL PADEVNMPHQ
410 420 430 440 450
WVEGNMPVSS QCAVCHESCG SYQRLQDFRC LWCNSTVHDD CRRRFSKECC
460 470 480 490 500
FRSHRSSVIP PTALSDPKGD GQLVVSSDFW NLDWSSACSC PLLIFINSKS
510 520 530 540 550
GDHQGIVFLR KFKQYLNPSQ VFDLLKGGPE AGLSMFKNFA RFRILVCGGD
560 570 580 590 600
GSVSWVLSLI DAFGLHEKCQ LAVIPLGTGN DLARVLGWGA FWNKSKSPLD
610 620 630 640 650
ILNRVEQASV RILDRWSVMI RETPRQTPLL KGQVEMDVPR FEAAAIQHLE
660 670 680 690 700
SAATELNKIL KAKYPTEMII ATRFLCSAVE DFVVDIVKAW GQIKQNNTAI
710 720 730 740 750
VSVILKSDLM YDRLSVLIDV LAEEAAATSA EKSATEYADS SKADRKPFIP
760 770 780 790 800
QIDHIAKCKL ELATKAQSLQ KSLKLIIFQV EQALDEESRQ TISVKNFSST
810 820 830 840 850
FFLEDDPEDI NQTSPRRRSR RGTLSSISSL KSEDLDNLNL DHLHFTPESI
860 870 880 890 900
RFKEKCVMNN YFGIGLDAKI SLDFNTRRDE HPGQYNSRLK NKMWYGLLGT
910 920 930 940 950
KELLQRSYRK LEERVHLECD GETISLPNLQ GIVVLNITSY AGGINFWGSN
960 970 980 990 1000
TATTEYEAPA IDDGKLEVVA IFGSVQMAMS RIINLHHHRI AQCHEVMITI
1010 1020 1030 1040 1050
DGEEGIPVQV DGEAWIQRPG LIKIRYKNAA QMLTRDRDFE NSMKMWEYKH
1060 1070 1080 1090 1100
TEIQAAPQPQ LDFQDSQESL SDEEYAQMQH LARLAENLIS KLNDLSKIHQ
1110 1120 1130 1140 1150
HVSVLMGSVN ASANILNDIF YGQDSGNEMG AASCIPIETL SRNDAVDVTF
1160 1170 1180 1190 1200
SLKGLYDDTT AFLDEKLLRS AEDETALQSA LDAMNKEFKK LSEIDWMNPI
1210 1220 1230 1240 1250
FVPEEKSSDT DSRSLRLKIK FPKLGKKKVE EERKPKSGQS VQSFIGNLWH
1260 1270
RRHREDEAEG DDPLTPSRSQ L
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048859 | 1118 | D → N. Corresponds to variant dbSNP:rs4074320Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB183864 mRNA Translation: BAD86792.1 BC137319 mRNA Translation: AAI37320.1 BC137320 mRNA Translation: AAI37321.1 |
CCDSi | CCDS75980.1 |
RefSeqi | NP_001013764.1, NM_001013742.3 |
Genome annotation databases
Ensembli | ENST00000611977; ENSP00000477515; ENSG00000274588 |
GeneIDi | 139189 |
KEGGi | hsa:139189 |
UCSCi | uc033edr.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB183864 mRNA Translation: BAD86792.1 BC137319 mRNA Translation: AAI37320.1 BC137320 mRNA Translation: AAI37321.1 |
CCDSi | CCDS75980.1 |
RefSeqi | NP_001013764.1, NM_001013742.3 |
3D structure databases
SMRi | Q5KSL6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 126548, 1 interactor |
IntActi | Q5KSL6, 1 interactor |
STRINGi | 9606.ENSP00000477515 |
Chemistry databases
DrugBanki | DB14001, alpha-Tocopherol succinate |
SwissLipidsi | SLP:000000926 |
PTM databases
iPTMneti | Q5KSL6 |
PhosphoSitePlusi | Q5KSL6 |
Genetic variation databases
BioMutai | DGKK |
DMDMi | 74708075 |
Proteomic databases
EPDi | Q5KSL6 |
MassIVEi | Q5KSL6 |
MaxQBi | Q5KSL6 |
PeptideAtlasi | Q5KSL6 |
PRIDEi | Q5KSL6 |
ProteomicsDBi | 63549 |
Protocols and materials databases
Antibodypediai | 72568, 128 antibodies |
Genome annotation databases
Ensembli | ENST00000611977; ENSP00000477515; ENSG00000274588 |
GeneIDi | 139189 |
KEGGi | hsa:139189 |
UCSCi | uc033edr.2, human |
Organism-specific databases
CTDi | 139189 |
DisGeNETi | 139189 |
GeneCardsi | DGKK |
HGNCi | HGNC:32395, DGKK |
HPAi | ENSG00000274588, Group enriched (adrenal gland, brain, pituitary gland) |
MIMi | 300837, gene |
neXtProti | NX_Q5KSL6 |
OpenTargetsi | ENSG00000274588 |
PharmGKBi | PA142671978 |
VEuPathDBi | HostDB:ENSG00000274588.1 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1170, Eukaryota |
GeneTreei | ENSGT00940000162262 |
HOGENOMi | CLU_001799_3_3_1 |
InParanoidi | Q5KSL6 |
OMAi | ECKHTEI |
OrthoDBi | 43384at2759 |
PhylomeDBi | Q5KSL6 |
Enzyme and pathway databases
UniPathwayi | UPA00230 |
PathwayCommonsi | Q5KSL6 |
Reactomei | R-HSA-114508, Effects of PIP2 hydrolysis |
Miscellaneous databases
BioGRID-ORCSi | 139189, 5 hits in 131 CRISPR screens |
ChiTaRSi | DGKK, human |
GenomeRNAii | 139189 |
Pharosi | Q5KSL6, Tbio |
PROi | PR:Q5KSL6 |
RNActi | Q5KSL6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000274588, Expressed in metanephros and 55 other tissues |
Genevisiblei | Q5KSL6, HS |
Family and domain databases
CDDi | cd00029, C1, 2 hits |
Gene3Di | 2.30.29.30, 1 hit 3.40.50.10330, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR037607, DGK IPR000756, Diacylglycerol_kin_accessory IPR001206, Diacylglycerol_kinase_cat_dom IPR016064, NAD/diacylglycerol_kinase_sf IPR002219, PE/DAG-bd IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR11255, PTHR11255, 1 hit |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF00609, DAGK_acc, 1 hit PF00781, DAGK_cat, 1 hit PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 2 hits SM00045, DAGKa, 1 hit SM00046, DAGKc, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
PROSITEi | View protein in PROSITE PS50146, DAGK, 1 hit PS50003, PH_DOMAIN, 1 hit PS00479, ZF_DAG_PE_1, 2 hits PS50081, ZF_DAG_PE_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DGKK_HUMAN | |
Accessioni | Q5KSL6Primary (citable) accession number: Q5KSL6 Secondary accession number(s): B2RP91 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 13, 2006 |
Last sequence update: | February 15, 2005 | |
Last modified: | April 7, 2021 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families