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Protein

Thiamine pyrophosphokinase 1

Gene

TPK1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in glycolysis and energy production. Plant leaves require high levels of TPP for photosynthesis and carbohydrate metabolism (By similarity).By similarity

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine pyrophosphokinase 1 (TPK1), Thiamine pyrophosphokinase 2 (TPK2), Thiamine pyrophosphokinase (OsJ_01724), Thiamine pyrophosphokinase 3 (TPK3)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-196819 Vitamin B1 (thiamin) metabolism
UniPathwayiUPA00060; UER00597

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase 1 (EC:2.7.6.2)
Short name:
OsTPK1
Alternative name(s):
Thiamine kinase 1
Gene namesi
Name:TPK1
Ordered Locus Names:Os01g0931400, LOC_Os01g70580
ORF Names:OSJNBa0052O12.42-1, P0506E04.20-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004239681 – 264Thiamine pyrophosphokinase 1Add BLAST264

Proteomic databases

PaxDbiQ5JK24

Expressioni

Gene expression databases

ExpressionAtlasiQ5JK24 differential
GenevisibleiQ5JK24 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g70580.1

Structurei

3D structure databases

ProteinModelPortaliQ5JK24
SMRiQ5JK24
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiKOG3153 Eukaryota
COG1564 LUCA
InParanoidiQ5JK24
KOiK00949
OMAiHEIRIER
OrthoDBiEOG09360JCF

Family and domain databases

CDDicd07995 TPK, 1 hit
Gene3Di3.40.50.10240, 1 hit
InterProiView protein in InterPro
IPR006282 Thi_PPkinase
IPR016966 Thiamin_pyrophosphokinase_euk
IPR007373 Thiamin_PyroPKinase_B1-bd
IPR036371 TPK_B1-bd_sf
IPR007371 TPK_catalytic
IPR036759 TPK_catalytic_sf
PfamiView protein in Pfam
PF04265 TPK_B1_binding, 1 hit
PF04263 TPK_catalytic, 1 hit
PIRSFiPIRSF031057 Thiamin_pyrophosphokinase, 1 hit
SMARTiView protein in SMART
SM00983 TPK_B1_binding, 1 hit
SUPFAMiSSF63862 SSF63862, 1 hit
SSF63999 SSF63999, 1 hit
TIGRFAMsiTIGR01378 thi_PPkinase, 1 hit

Sequencei

Sequence statusi: Complete.

Q5JK24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPTMTHSS SFLRLPATSS PHPPPADDAS AAYAVVVLNQ RLPRFAPLLW
60 70 80 90 100
DRARLRVCAD GGANRVFDGM PELLPAEDPD QVRMRYKPDV IKGDMDSIRP
110 120 130 140 150
EVKEYYSNLG AEIVDESHDQ DTTDLHKCVS FITRNPPGSE ESNLYILVLG
160 170 180 190 200
ALGGRFDHEM GNINVLYRFS NIRIVLLSDD CSIFLLPKTH SHEIHIERSI
210 220 230 240 250
EGPHCGLIPM GSPSASTTTT GLRWNLDNTS MSYGGLISTS NIVEEETVRI
260
TSDSDLIWTI SLRN
Length:264
Mass (Da):29,288
Last modified:February 15, 2005 - v1
Checksum:iD5D19BD6102E2A07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003272 Genomic DNA Translation: BAD87327.1
AP004330 Genomic DNA Translation: BAD88183.1
AP008207 Genomic DNA Translation: BAF07211.1
AP014957 Genomic DNA Translation: BAS76043.1
AK105048 mRNA Translation: BAG97081.1
RefSeqiXP_015621775.1, XM_015766289.1
UniGeneiOs.52120

Genome annotation databases

EnsemblPlantsiOs01t0931400-02; Os01t0931400-02; Os01g0931400
GeneIDi4326818
GrameneiOs01t0931400-02; Os01t0931400-02; Os01g0931400
KEGGiosa:4326818

Similar proteinsi

Entry informationi

Entry nameiTPK1_ORYSJ
AccessioniPrimary (citable) accession number: Q5JK24
Secondary accession number(s): A0A0P0VCI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: February 15, 2005
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

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