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Entry version 100 (07 Apr 2021)
Sequence version 1 (15 Feb 2005)
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Protein

NAD(P)H sulfur oxidoreductase (CoA-dependent)

Gene

TK1299

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the CoA-dependent reduction of elemental sulfur (S0) to produce hydrogen sulfide. Can use both NADPH and NADH, but shows a preference for NADPH (PubMed:24723088).

CoA-SH may act to solubilize S0 by forming CoA persulfides, followed by the reduction of an enzyme-S-S-CoA intermediate produced after both enzymatic and nonenzymatic evolution of H2S from the CoA persulfide (PubMed:24723088).

Also catalyzes the oxidation of NADPH as well as NADH and predominantly converts O2 to H2O, with a preference for NADPH (PubMed:23453203).

2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

FAD2 PublicationsNote: Binds 1 FAD per subunit.By similarity

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 68 sec(-1) for NADH oxidase activity. kcat is 50 sec(-1) for NADPH oxidase activity.1 Publication
  1. KM=24 µM for NADH1 Publication
  2. KM=25 µM for NADPH1 Publication
  3. KM=260 µM for CoA-SH1 Publication
  1. Vmax=83 µmol/min/mg enzyme for NADH oxidase activity1 Publication
  2. Vmax=61 µmol/min/mg enzyme for NADPH oxidase activity1 Publication

pH dependencei

Optimum pH is 8.0 for NAD(P)H oxidase activity.1 Publication

Temperature dependencei

Highly thermostable.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei24Coenzyme ABy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei45Redox-activeBy similarity1
Binding sitei72Coenzyme ABy similarity1
Binding sitei82FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei280FADBy similarity1
Binding sitei298FAD; via amide nitrogenBy similarity1
Binding sitei302Coenzyme ABy similarity1
Binding sitei358Coenzyme A; via carbonyl oxygenBy similarity1
Binding sitei422FAD; via carbonyl oxygenBy similarity1
Binding sitei430Coenzyme ABy similarity1
Binding sitei438Coenzyme ABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi13 – 14FADBy similarity2
Nucleotide bindingi35 – 36FADBy similarity2
Nucleotide bindingi42 – 44FADBy similarity3

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
TKOD69014:G1G2A-1294-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.6.3.1, 5246
1.6.3.2, 5246
1.8.1.18, 5246

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
NAD(P)H sulfur oxidoreductase (CoA-dependent)Curated (EC:1.8.1.181 Publication)
Short name:
NSR1 Publication
Alternative name(s):
NAD(P)H oxidase (H2O-forming)Curated (EC:1.6.3.21 Publication)
Short name:
NOX1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Ordered Locus Names:TK1299
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri69014 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000536 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001847001 – 442NAD(P)H sulfur oxidoreductase (CoA-dependent)Add BLAST442

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homohexamer.

2 Publications

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
Q5JGP4, 1 interactor

Molecular INTeraction database

More...
MINTi
Q5JGP4

STRING: functional protein association networks

More...
STRINGi
69014.TK1299

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q5JGP4

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni41 – 45Coenzyme A bindingBy similarity5
Regioni62 – 63Coenzyme A bindingBy similarity2

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
arCOG01069, Archaea

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_003291_1_3_2

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q5JGP4

Identification of Orthologs from Complete Genome Data

More...
OMAi
WVSHAPC

Database of Orthologous Groups

More...
OrthoDBi
40511at2157

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.50.50.60, 3 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR017758, CoA_disulphide_reductase
IPR036188, FAD/NAD-bd_sf
IPR023753, FAD/NAD-binding_dom
IPR016156, FAD/NAD-linked_Rdtase_dimer_sf
IPR004099, Pyr_nucl-diS_OxRdtase_dimer

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07992, Pyr_redox_2, 1 hit
PF02852, Pyr_redox_dim, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51905, SSF51905, 1 hit
SSF55424, SSF55424, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR03385, CoA_CoA_reduc, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q5JGP4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERKTVVVIG GGAAGMSTAS RVKRLKPEWD VKVFEATEWV SHAPCGIPYV
60 70 80 90 100
VEGISPKEKL MHYPPEVFIK KRGIDLHLKA EVIEVEQGRV RVREEDGEKT
110 120 130 140 150
YEWDYLVFAN GASPQVPAIE GIDLPGVFTA DLPPDAVAIT EYLEKNPVEN
160 170 180 190 200
VVVIGTGYIA IEMAEAFVER GKNVTLIGRS ERVLRKTFDK EITDIVEEKL
210 220 230 240 250
RNHLNLRLEE VTLRIEGKER VERVVTDAGE YPADLVIVAT GIKPNTELAR
260 270 280 290 300
GLGVRIGETG AIWTNDRMQT SVENVYAAGD VAETKHLITG RRVWMPLAPA
310 320 330 340 350
GNKMGYVAGS NIAGKEIHFP GVLGTSITKF LDLEIGKTGL TEAEAMKEGY
360 370 380 390 400
DVRTAFIKAG TRPHYYPGSK TIWLKGVVDN ETNRLLGVQA VGGDILPRID
410 420 430 440
TAAAMITAGF TTKDVFFTDL AYAPPFAPVW DPLIVLARVL KF
Length:442
Mass (Da):48,610
Last modified:February 15, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA51CC778EA62E476
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AP006878 Genomic DNA Translation: BAD85488.1

NCBI Reference Sequences

More...
RefSeqi
WP_011250250.1, NC_006624.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
BAD85488; BAD85488; TK1299

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
3235301

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
tko:TK1299

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|69014.16.peg.1271

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA Translation: BAD85488.1
RefSeqiWP_011250250.1, NC_006624.1

3D structure databases

SMRiQ5JGP4
ModBaseiSearch...

Protein-protein interaction databases

IntActiQ5JGP4, 1 interactor
MINTiQ5JGP4
STRINGi69014.TK1299

Genome annotation databases

EnsemblBacteriaiBAD85488; BAD85488; TK1299
GeneIDi3235301
KEGGitko:TK1299
PATRICifig|69014.16.peg.1271

Phylogenomic databases

eggNOGiarCOG01069, Archaea
HOGENOMiCLU_003291_1_3_2
InParanoidiQ5JGP4
OMAiWVSHAPC
OrthoDBi40511at2157

Enzyme and pathway databases

BioCyciTKOD69014:G1G2A-1294-MONOMER
BRENDAi1.6.3.1, 5246
1.6.3.2, 5246
1.8.1.18, 5246

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR017758, CoA_disulphide_reductase
IPR036188, FAD/NAD-bd_sf
IPR023753, FAD/NAD-binding_dom
IPR016156, FAD/NAD-linked_Rdtase_dimer_sf
IPR004099, Pyr_nucl-diS_OxRdtase_dimer
PfamiView protein in Pfam
PF07992, Pyr_redox_2, 1 hit
PF02852, Pyr_redox_dim, 1 hit
SUPFAMiSSF51905, SSF51905, 1 hit
SSF55424, SSF55424, 1 hit
TIGRFAMsiTIGR03385, CoA_CoA_reduc, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNSR_THEKO
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q5JGP4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: February 15, 2005
Last modified: April 7, 2021
This is version 100 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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