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UniProtKB - Q5JCS9 (B3GN3_MOUSE)
Protein
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3
Gene
B3gnt3
Organism
Mus musculus (Mouse)
Status
Functioni
Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides. Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans.
By similarityCatalytic activityi
- 3-O-{β-D-galactosyl-(1→3)-[N-acetyl-β-D-glucosaminyl-(1→6)]-N-acetyl-α-D-galactosaminyl}-L-threonyl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-{β-D-GlcNAc-(1→3)-β-D-Gal-(1→3)-[β-D-GlcNAc-(1→6)]-α-D-GalNAc}-L-threonyl-[protein] + H+ + UDPBy similarityEC:2.4.1.146By similarity
- 3-O-{β-D-galactosyl-(1→3)-[N-acetyl-β-D-glucosaminyl-(1→6)]-N-acetyl-α-D-galactosaminyl}-L-seryl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-{β-D-GlcNAc-(1→3)-β-D-Gal-(1→3)-[β-D-GlcNAc-(1→6)]-α-D-GalNAc}-L-seryl-[protein] + H+ + UDPBy similarityEC:2.4.1.146By similarity
- a β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + UDP-N-acetyl-α-D-glucosamine = an N-acetyl-β-D-glucosaminyl-(1→3)-β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + H+ + UDPBy similarityEC:2.4.1.149By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
- beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity Source: UniProtKB-EC
- glycosyltransferase activity Source: GO_Central
- N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Source: UniProtKB
- UDP-glycosyltransferase activity Source: GO_Central
GO - Biological processi
- poly-N-acetyllactosamine biosynthetic process Source: UniProtKB
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-MMU-2022854, Keratan sulfate biosynthesis R-MMU-913709, O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT31, Glycosyltransferase Family 31 |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 (EC:2.4.1.149By similarity)Alternative name(s): Core 1 extending beta-1,3-N-acetylglucosaminyltransferaseBy similarity (EC:2.4.1.146By similarity) Core1-beta3GlcNAcT UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 Short name: BGnT-3 Short name: Beta-1,3-Gn-T3 Short name: Beta-1,3-N-acetylglucosaminyltransferase 3 Short name: Beta3Gn-T3 |
Gene namesi | Name:B3gnt3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2152535, B3gnt3 |
VEuPathDBi | HostDB:ENSMUSG00000031803 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane Curated; Single-pass type II membrane protein Curated
Golgi apparatus
- Golgi membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 10 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 11 – 31 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 32 – 372 | LumenalSequence analysisAdd BLAST | 341 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219173 | 1 – 372 | N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3Add BLAST | 372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 64 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 184 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 202 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | Q5JCS9 |
PaxDbi | Q5JCS9 |
PRIDEi | Q5JCS9 |
ProteomicsDBi | 265189 |
PTM databases
GlyGeni | Q5JCS9, 3 sites |
PhosphoSitePlusi | Q5JCS9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031803, Expressed in stomach and 98 other tissues |
Genevisiblei | Q5JCS9, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034260 |
Miscellaneous databases
RNActi | Q5JCS9, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 31 – 56 | DisorderedSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Belongs to the glycosyltransferase 31 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2287, Eukaryota |
GeneTreei | ENSGT00940000159134 |
HOGENOMi | CLU_036849_5_3_1 |
InParanoidi | Q5JCS9 |
OMAi | YCAGGGF |
OrthoDBi | 1037602at2759 |
PhylomeDBi | Q5JCS9 |
TreeFami | TF318639 |
Family and domain databases
InterProi | View protein in InterPro IPR002659, Glyco_trans_31 |
PANTHERi | PTHR11214, PTHR11214, 1 hit |
Pfami | View protein in Pfam PF01762, Galactosyl_T, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5JCS9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLPRQSPYE ILLLVLVALL VLLLLLTSKS PPSCSAPERS KEPEDNPGWA
60 70 80 90 100
TGHPRTHPAR CRANLSVSSH PDFAGLPLHV RDFLFYRHCR DFPVLREPRV
110 120 130 140 150
TKCAEPVFLL LAIKSSPANY GRRQMLRTTW ARERRVRGAP LRRLFLVGSD
160 170 180 190 200
RDPQQARKYN RLLELEAQKY GDILQWDFHD SFFNLTLKQV LFLEWQLTYC
210 220 230 240 250
TNASFVLNGD DDVFAHTDNM VTYLQDHDPD QHLFVGHLIQ NVGPIRVPWS
260 270 280 290 300
KYFIPALVMA EDRYPPYCGG GGFLLSRFTV AALRRAARVL PMFPIDDVFL
310 320 330 340 350
GMCLQQQGLA PGTHSGVRTA GVFPPSPRVS SFDPCFYRDL LLVHRFLPFE
360 370
MLLMWDALNQ PQLLCGRQSP AY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 256 | A → T in AAH26418 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 283 | L → I in AAK68855 (Ref. 1) Curated | 1 | |
Sequence conflicti | 283 | L → I in BAB25824 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 295 | I → V in AAK68855 (Ref. 1) Curated | 1 | |
Sequence conflicti | 295 | I → V in BAB25824 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY037785 mRNA Translation: AAK68855.1 AY039028 mRNA Translation: AAK72481.1 AK008674 mRNA Translation: BAB25824.1 BC026418 mRNA Translation: AAH26418.1 |
CCDSi | CCDS22405.1 |
RefSeqi | NP_082465.3, NM_028189.3 XP_006509815.1, XM_006509752.3 XP_006509816.1, XM_006509753.3 |
Genome annotation databases
Ensembli | ENSMUST00000034260; ENSMUSP00000034260; ENSMUSG00000031803 |
GeneIDi | 72297 |
KEGGi | mmu:72297 |
UCSCi | uc009mer.3, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase beta 3 GlcNAc-T III |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY037785 mRNA Translation: AAK68855.1 AY039028 mRNA Translation: AAK72481.1 AK008674 mRNA Translation: BAB25824.1 BC026418 mRNA Translation: AAH26418.1 |
CCDSi | CCDS22405.1 |
RefSeqi | NP_082465.3, NM_028189.3 XP_006509815.1, XM_006509752.3 XP_006509816.1, XM_006509753.3 |
3D structure databases
SMRi | Q5JCS9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034260 |
Protein family/group databases
CAZyi | GT31, Glycosyltransferase Family 31 |
PTM databases
GlyGeni | Q5JCS9, 3 sites |
PhosphoSitePlusi | Q5JCS9 |
Proteomic databases
MaxQBi | Q5JCS9 |
PaxDbi | Q5JCS9 |
PRIDEi | Q5JCS9 |
ProteomicsDBi | 265189 |
Protocols and materials databases
Antibodypediai | 14471, 104 antibodies from 23 providers |
DNASUi | 72297 |
Genome annotation databases
Ensembli | ENSMUST00000034260; ENSMUSP00000034260; ENSMUSG00000031803 |
GeneIDi | 72297 |
KEGGi | mmu:72297 |
UCSCi | uc009mer.3, mouse |
Organism-specific databases
CTDi | 10331 |
MGIi | MGI:2152535, B3gnt3 |
VEuPathDBi | HostDB:ENSMUSG00000031803 |
Phylogenomic databases
eggNOGi | KOG2287, Eukaryota |
GeneTreei | ENSGT00940000159134 |
HOGENOMi | CLU_036849_5_3_1 |
InParanoidi | Q5JCS9 |
OMAi | YCAGGGF |
OrthoDBi | 1037602at2759 |
PhylomeDBi | Q5JCS9 |
TreeFami | TF318639 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-MMU-2022854, Keratan sulfate biosynthesis R-MMU-913709, O-linked glycosylation of mucins |
Miscellaneous databases
BioGRID-ORCSi | 72297, 1 hit in 65 CRISPR screens |
PROi | PR:Q5JCS9 |
RNActi | Q5JCS9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031803, Expressed in stomach and 98 other tissues |
Genevisiblei | Q5JCS9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR002659, Glyco_trans_31 |
PANTHERi | PTHR11214, PTHR11214, 1 hit |
Pfami | View protein in Pfam PF01762, Galactosyl_T, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | B3GN3_MOUSE | |
Accessioni | Q5JCS9Primary (citable) accession number: Q5JCS9 Secondary accession number(s): Q8R0U2, Q9D7Z2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | February 15, 2005 | |
Last modified: | February 23, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families