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Protein

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3

Gene

B3gnt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides. Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + 3-O-(beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein] = UDP + 3-O-(N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein].By similarity
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-2022854 Keratan sulfate biosynthesis
R-MMU-913709 O-linked glycosylation of mucins
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 (EC:2.4.1.149By similarity)
Alternative name(s):
Core 1 extending beta-1,3-N-acetylglucosaminyltransferaseBy similarity (EC:2.4.1.146By similarity)
Core1-beta3GlcNAcT
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Short name:
BGnT-3
Short name:
Beta-1,3-Gn-T3
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 3
Short name:
Beta3Gn-T3
Gene namesi
Name:B3gnt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2152535 B3gnt3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10CytoplasmicSequence analysis10
Transmembranei11 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini32 – 372LumenalSequence analysisAdd BLAST341

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191731 – 372N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi184N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi202N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ5JCS9
PaxDbiQ5JCS9
PRIDEiQ5JCS9

PTM databases

PhosphoSitePlusiQ5JCS9

Expressioni

Gene expression databases

BgeeiENSMUSG00000031803 Expressed in 60 organ(s), highest expression level in jejunum
GenevisibleiQ5JCS9 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034260

Structurei

3D structure databases

ProteinModelPortaliQ5JCS9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287 Eukaryota
ENOG410ZZ1B LUCA
GeneTreeiENSGT00760000118879
HOGENOMiHOG000232195
HOVERGENiHBG050653
InParanoidiQ5JCS9
KOiK07970
OMAiYKHCRDF
OrthoDBiEOG091G0AXM
PhylomeDBiQ5JCS9
TreeFamiTF318639

Family and domain databases

InterProiView protein in InterPro
IPR002659 Glyco_trans_31
PANTHERiPTHR11214 PTHR11214, 1 hit
PfamiView protein in Pfam
PF01762 Galactosyl_T, 1 hit

Sequencei

Sequence statusi: Complete.

Q5JCS9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLPRQSPYE ILLLVLVALL VLLLLLTSKS PPSCSAPERS KEPEDNPGWA
60 70 80 90 100
TGHPRTHPAR CRANLSVSSH PDFAGLPLHV RDFLFYRHCR DFPVLREPRV
110 120 130 140 150
TKCAEPVFLL LAIKSSPANY GRRQMLRTTW ARERRVRGAP LRRLFLVGSD
160 170 180 190 200
RDPQQARKYN RLLELEAQKY GDILQWDFHD SFFNLTLKQV LFLEWQLTYC
210 220 230 240 250
TNASFVLNGD DDVFAHTDNM VTYLQDHDPD QHLFVGHLIQ NVGPIRVPWS
260 270 280 290 300
KYFIPALVMA EDRYPPYCGG GGFLLSRFTV AALRRAARVL PMFPIDDVFL
310 320 330 340 350
GMCLQQQGLA PGTHSGVRTA GVFPPSPRVS SFDPCFYRDL LLVHRFLPFE
360 370
MLLMWDALNQ PQLLCGRQSP AY
Length:372
Mass (Da):42,586
Last modified:February 15, 2005 - v1
Checksum:i484771C8054DE265
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256A → T in AAH26418 (PubMed:15489334).Curated1
Sequence conflicti283L → I in AAK68855 (Ref. 1) Curated1
Sequence conflicti283L → I in BAB25824 (PubMed:16141072).Curated1
Sequence conflicti295I → V in AAK68855 (Ref. 1) Curated1
Sequence conflicti295I → V in BAB25824 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037785 mRNA Translation: AAK68855.1
AY039028 mRNA Translation: AAK72481.1
AK008674 mRNA Translation: BAB25824.1
BC026418 mRNA Translation: AAH26418.1
CCDSiCCDS22405.1
RefSeqiNP_082465.3, NM_028189.3
XP_006509815.1, XM_006509752.3
XP_006509816.1, XM_006509753.3
UniGeneiMm.290837

Genome annotation databases

EnsembliENSMUST00000034260; ENSMUSP00000034260; ENSMUSG00000031803
GeneIDi72297
KEGGimmu:72297
UCSCiuc009mer.3 mouse

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

beta 3 GlcNAc-T III

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037785 mRNA Translation: AAK68855.1
AY039028 mRNA Translation: AAK72481.1
AK008674 mRNA Translation: BAB25824.1
BC026418 mRNA Translation: AAH26418.1
CCDSiCCDS22405.1
RefSeqiNP_082465.3, NM_028189.3
XP_006509815.1, XM_006509752.3
XP_006509816.1, XM_006509753.3
UniGeneiMm.290837

3D structure databases

ProteinModelPortaliQ5JCS9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034260

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

PTM databases

PhosphoSitePlusiQ5JCS9

Proteomic databases

MaxQBiQ5JCS9
PaxDbiQ5JCS9
PRIDEiQ5JCS9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034260; ENSMUSP00000034260; ENSMUSG00000031803
GeneIDi72297
KEGGimmu:72297
UCSCiuc009mer.3 mouse

Organism-specific databases

CTDi10331
MGIiMGI:2152535 B3gnt3

Phylogenomic databases

eggNOGiKOG2287 Eukaryota
ENOG410ZZ1B LUCA
GeneTreeiENSGT00760000118879
HOGENOMiHOG000232195
HOVERGENiHBG050653
InParanoidiQ5JCS9
KOiK07970
OMAiYKHCRDF
OrthoDBiEOG091G0AXM
PhylomeDBiQ5JCS9
TreeFamiTF318639

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-MMU-2022854 Keratan sulfate biosynthesis
R-MMU-913709 O-linked glycosylation of mucins

Miscellaneous databases

PROiPR:Q5JCS9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031803 Expressed in 60 organ(s), highest expression level in jejunum
GenevisibleiQ5JCS9 MM

Family and domain databases

InterProiView protein in InterPro
IPR002659 Glyco_trans_31
PANTHERiPTHR11214 PTHR11214, 1 hit
PfamiView protein in Pfam
PF01762 Galactosyl_T, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiB3GN3_MOUSE
AccessioniPrimary (citable) accession number: Q5JCS9
Secondary accession number(s): Q8R0U2, Q9D7Z2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: February 15, 2005
Last modified: September 12, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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