B3gnt3

Functionⁱ

Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides. Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans.By similarity

Catalytic activityⁱ

UDP-N-acetyl-alpha-D-glucosamine + 3-O-(beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein] = UDP + 3-O-(N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein].By similarity

UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R.By similarity

Pathwayⁱ: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functionⁱ

beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
galactosyltransferase activity Source: InterPro
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

poly-N-acetyllactosamine biosynthetic process Source: UniProtKB
protein glycosylation Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase

Molecular function

Glycosyltransferase, Transferase

Enzyme and pathway databases

Reactomeⁱ	R-MMU-2022854 Keratan sulfate biosynthesis R-MMU-913709 O-linked glycosylation of mucins
UniPathwayⁱ	UPA00378

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT31 Glycosyltransferase Family 31

CAZyⁱ

GT31 Glycosyltransferase Family 31

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 (EC:2.4.1.149By similarity) Alternative name(s): Core 1 extending beta-1,3-N-acetylglucosaminyltransferaseBy similarity (EC:2.4.1.146By similarity) Core1-beta3GlcNAcT UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 Short name: BGnT-3 Short name: Beta-1,3-Gn-T3 Short name: Beta-1,3-N-acetylglucosaminyltransferase 3 Short name: Beta3Gn-T3
Gene namesⁱ	Name:B3gnt3
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 8

Organism-specific databases

MGIⁱ	MGI:2152535 B3gnt3

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 10	CytoplasmicSequence analysis	10
Transmembraneⁱ	11 – 31	Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST	21
Topological domainⁱ	32 – 372	LumenalSequence analysisAdd BLAST	341

Keywords - Cellular componentⁱ

Golgi apparatus, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000219173	1 – 372	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3Add BLAST		372

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	64	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	184	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	202	N-linked (GlcNAc...) asparagineSequence analysis	1

Keywords - PTMⁱ

Glycoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q5JCS9
PaxDbⁱ	Q5JCS9
PRIDEⁱ	Q5JCS9

PTM databases

PhosphoSitePlusⁱ	Q5JCS9

PhosphoSitePlusⁱ

Q5JCS9

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000031803 Expressed in 60 organ(s), highest expression level in jejunum
Genevisibleⁱ	Q5JCS9 MM

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000034260

STRINGⁱ

10090.ENSMUSP00000034260

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q5JCS9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domainⁱ

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG2287 Eukaryota ENOG410ZZ1B LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118879
HOGENOMⁱ	HOG000232195
HOVERGENⁱ	HBG050653
InParanoidⁱ	Q5JCS9
KEGG Orthology (KO) More...KOⁱ	K07970
OMAⁱ	YKHCRDF
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0AXM
PhylomeDBⁱ	Q5JCS9
TreeFamⁱ	TF318639

Family and domain databases

InterProⁱ	View protein in InterPro IPR002659 Glyco_trans_31
PANTHERⁱ	PTHR11214 PTHR11214, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01762 Galactosyl_T, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q5JCS9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRLPRQSPYE ILLLVLVALL VLLLLLTSKS PPSCSAPERS KEPEDNPGWA 
        60         70         80         90        100
TGHPRTHPAR CRANLSVSSH PDFAGLPLHV RDFLFYRHCR DFPVLREPRV 
       110        120        130        140        150
TKCAEPVFLL LAIKSSPANY GRRQMLRTTW ARERRVRGAP LRRLFLVGSD 
       160        170        180        190        200
RDPQQARKYN RLLELEAQKY GDILQWDFHD SFFNLTLKQV LFLEWQLTYC 
       210        220        230        240        250
TNASFVLNGD DDVFAHTDNM VTYLQDHDPD QHLFVGHLIQ NVGPIRVPWS 
       260        270        280        290        300
KYFIPALVMA EDRYPPYCGG GGFLLSRFTV AALRRAARVL PMFPIDDVFL 
       310        320        330        340        350
GMCLQQQGLA PGTHSGVRTA GVFPPSPRVS SFDPCFYRDL LLVHRFLPFE 
       360        370 
MLLMWDALNQ PQLLCGRQSP AY

Length:372

Mass (Da):42,586

Last modified:February 15, 2005 - v1

Checksum:ⁱ484771C8054DE265

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	256	A → T in AAH26418 (PubMed:15489334).Curated	1
Sequence conflictⁱ	283	L → I in AAK68855 (Ref. 1) Curated	1
Sequence conflictⁱ	283	L → I in BAB25824 (PubMed:16141072).Curated	1
Sequence conflictⁱ	295	I → V in AAK68855 (Ref. 1) Curated	1
Sequence conflictⁱ	295	I → V in BAB25824 (PubMed:16141072).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY037785 mRNA Translation: AAK68855.1 AY039028 mRNA Translation: AAK72481.1 AK008674 mRNA Translation: BAB25824.1 BC026418 mRNA Translation: AAH26418.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS22405.1
NCBI Reference Sequences More...RefSeqⁱ	NP_082465.3, NM_028189.3 XP_006509815.1, XM_006509752.3 XP_006509816.1, XM_006509753.3
UniGeneⁱ	Mm.290837

Genome annotation databases

Ensemblⁱ	ENSMUST00000034260; ENSMUSP00000034260; ENSMUSG00000031803
GeneIDⁱ	72297
KEGGⁱ	mmu:72297
UCSC genome browser More...UCSCⁱ	uc009mer.3 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q5JCS9	Hexosyltransferase		MOUSE		372	UniRef90_Q5JCS9
UPI000A314F4E		MUSCR		373
UPI000A30799F		MUSPA		372
Hexosyltransferase		MOUSE		372

Protein	Similar proteins		Species	Score	Length	Source
Q5JCS9	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3		HUMAN		372	UniRef50_Q9Y2A9
Hexosyltransferase		CERAT		373
Hexosyltransferase		MANLE		373
Hexosyltransferase		CHLSB		373
Hexosyltransferase		MACFA		373
+69

Cross-referencesⁱ

Web resourcesⁱ

Functional Glycomics Gateway - GTase

beta 3 GlcNAc-T III

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY037785 mRNA Translation: AAK68855.1 AY039028 mRNA Translation: AAK72481.1 AK008674 mRNA Translation: BAB25824.1 BC026418 mRNA Translation: AAH26418.1
CCDSⁱ	CCDS22405.1
RefSeqⁱ	NP_082465.3, NM_028189.3 XP_006509815.1, XM_006509752.3 XP_006509816.1, XM_006509753.3
UniGeneⁱ	Mm.290837

3D structure databases

ProteinModelPortalⁱ	Q5JCS9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000034260

STRINGⁱ

10090.ENSMUSP00000034260

Protein family/group databases

CAZyⁱ	GT31 Glycosyltransferase Family 31

CAZyⁱ

GT31 Glycosyltransferase Family 31

PTM databases

PhosphoSitePlusⁱ	Q5JCS9

PhosphoSitePlusⁱ

Q5JCS9

Proteomic databases

MaxQBⁱ	Q5JCS9
PaxDbⁱ	Q5JCS9
PRIDEⁱ	Q5JCS9

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000034260; ENSMUSP00000034260; ENSMUSG00000031803
GeneIDⁱ	72297
KEGGⁱ	mmu:72297
UCSCⁱ	uc009mer.3 mouse

Organism-specific databases

CTDⁱ	10331
MGIⁱ	MGI:2152535 B3gnt3

Phylogenomic databases

eggNOGⁱ	KOG2287 Eukaryota ENOG410ZZ1B LUCA
GeneTreeⁱ	ENSGT00760000118879
HOGENOMⁱ	HOG000232195
HOVERGENⁱ	HBG050653
InParanoidⁱ	Q5JCS9
KOⁱ	K07970
OMAⁱ	YKHCRDF
OrthoDBⁱ	EOG091G0AXM
PhylomeDBⁱ	Q5JCS9
TreeFamⁱ	TF318639

Enzyme and pathway databases

UniPathwayⁱ	UPA00378
Reactomeⁱ	R-MMU-2022854 Keratan sulfate biosynthesis R-MMU-913709 O-linked glycosylation of mucins

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q5JCS9
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000031803 Expressed in 60 organ(s), highest expression level in jejunum
Genevisibleⁱ	Q5JCS9 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR002659 Glyco_trans_31
PANTHERⁱ	PTHR11214 PTHR11214, 1 hit
Pfamⁱ	View protein in Pfam PF01762 Galactosyl_T, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	B3GN3_MOUSE
Accessionⁱ	Q5JCS9Primary (citable) accession number: Q5JCS9 Secondary accession number(s): Q8R0U2, Q9D7Z2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
	Last sequence update:	February 15, 2005
	Last modified:	September 12, 2018
	This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q5JCS9 (B3GN3_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein glycosylation

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Golgi apparatus

Golgi apparatus

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

Pathwayⁱ: protein glycosylation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ