Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine protease inhibitor A3G

Gene

Serpina3g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine and cysteine protease inhibitor. Can inhibit lysosomal papain-like proteases including the cathepsins B, G, H, K, L and V. Ineffective against elastase, granzyme A, granzyme B, or caspases 3, 8 or 9. Inhibition of cytoplasmic cathepsin B following release from the lysosome may protect cells from apoptosis. This may facilitate the survival of progenitor T-cells and the subsequent development of long term memory CD8 T-cells.3 Publications

Miscellaneous

The single human alpha1-antichymotrypsin gene (SERPINA3) is represented by a cluster of 14 individual murine paralogs.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei371 – 372Reactive bondBy similarity2

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • apoptotic process Source: UniProtKB-KW
  • response to cytokine Source: MGI
  • response to peptide hormone Source: MGI

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor, Thiol protease inhibitor
Biological processAdaptive immunity, Apoptosis, Immunity

Protein family/group databases

MEROPSiI04.959

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor A3G
Short name:
Serpin A3G
Alternative name(s):
Serine protease inhibitor 2A
Short name:
Serpin 2A
Gene namesi
Name:Serpina3g
Synonyms:Spi2A
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:105046 Serpina3g

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000940981 – 440Serine protease inhibitor A3GAdd BLAST440

Proteomic databases

MaxQBiQ5I2A0
PaxDbiQ5I2A0
PeptideAtlasiQ5I2A0
PRIDEiQ5I2A0

PTM databases

iPTMnetiQ5I2A0
PhosphoSitePlusiQ5I2A0

Expressioni

Tissue specificityi

Expressed in bone marrow (particularly hematopoietic stem cells), heart, kidney, liver, lung, skeletal muscle, spleen, testis, thymus and T-cells.3 Publications

Developmental stagei

T-cell specific expression rises during the differentiation of CD8 T-cell progenitors into memory CD8 T-cells.1 Publication

Inductioni

Induction during apoptosis requires NF-kappa-B, a heterodimer of RELA- and NFKB1.1 Publication

Interactioni

Protein-protein interaction databases

IntActiQ5I2A0, 2 interactors
MINTiQ5I2A0
STRINGi10090.ENSMUSP00000041250

Structurei

3D structure databases

ProteinModelPortaliQ5I2A0
SMRiQ5I2A0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni357 – 382RCLAdd BLAST26

Domaini

The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable (By similarity). Variability within the reactive center loop (RCL) sequences of Serpina3 paralogs may determine target protease specificity.By similarity

Sequence similaritiesi

Belongs to the serpin family.Curated

Phylogenomic databases

HOGENOMiHOG000238521
HOVERGENiHBG005957
InParanoidiQ5I2A0
KOiK04525
PhylomeDBiQ5I2A0

Family and domain databases

InterProiView protein in InterPro
IPR023795 Serpin_CS
IPR023796 Serpin_dom
IPR000215 Serpin_fam
IPR036186 Serpin_sf
PANTHERiPTHR11461 PTHR11461, 1 hit
PfamiView protein in Pfam
PF00079 Serpin, 1 hit
SMARTiView protein in SMART
SM00093 SERPIN, 1 hit
SUPFAMiSSF56574 SSF56574, 1 hit
PROSITEiView protein in PROSITE
PS00284 SERPIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q5I2A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVSPAVFG CPDVTLGRNT AVREVQENVT SVDSLTLVSS NTDFAFSLYR
60 70 80 90 100
KLVLKNPDEN VVFSPFSICT ALALLSLGAK SNTLKEILEG LKFNLTETPE
110 120 130 140 150
PDIHQGFRYL LDLLSQPGNQ VQISTGSALF IEKHLQILAE FKEKARALYQ
160 170 180 190 200
AEAFTADFQQ PLKATKLIND YVSNHTQGKI KELISGLKES TLMVLVNYIY
210 220 230 240 250
FKGKWKNPFD PNDTFKSEFY LDEKRSVIVS MMKTGYLTTP YFRDEELSCT
260 270 280 290 300
VVELKYTGNA SAMFILPDQG RMQQVEASLQ PETLRKWKNS LKPRMIHELR
310 320 330 340 350
LPKFSISTDY SLEHILPELG IREVFSTQAD LSAITGTKDL RVSQVVHKAV
360 370 380 390 400
LDVAETGTEA AAATGMAGVG CCAVFDFLEI FFNRPFLMII SDTKAHIALF
410 420 430 440
MAKVTNPERS MNFPNGEGAS SQRLESKRLC FGDPLCLIGQ
Length:440
Mass (Da):49,021
Last modified:June 21, 2005 - v2
Checksum:i7F449368DC27DD05
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti328Q → H in AAW56612 (Ref. 1) Curated1
Sequence conflicti356T → K in AAW56612 (Ref. 1) Curated1
Sequence conflicti411M → T in AAW56612 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY862185 mRNA Translation: AAW56612.1
BC057144 mRNA Translation: AAH57144.1
RefSeqiNP_033277.1, NM_009251.1
UniGeneiMm.312628
Mm.483584
Mm.486434

Genome annotation databases

GeneIDi20715
KEGGimmu:20715
UCSCiuc007oxa.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSPA3G_MOUSE
AccessioniPrimary (citable) accession number: Q5I2A0
Secondary accession number(s): Q6PG99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: May 23, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health