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Protein

Transcription initiation factor TFIID subunit 3

Gene

Taf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri867 – 917PHD-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • p53 binding Source: BHF-UCL
  • protein heterodimerization activity Source: InterPro

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 3
Alternative name(s):
140 kDa TATA box-binding protein-associated factor
TBP-associated factor 3
Transcription initiation factor TFIID 140 kDa subunit
Short name:
TAF(II)140
Short name:
TAF140
Short name:
TAFII-140
Short name:
TAFII140
Gene namesi
Name:Taf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2388097 Taf3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002455291 – 932Transcription initiation factor TFIID subunit 3Add BLAST932

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei183PhosphoserineCombined sources1
Modified residuei199PhosphoserineBy similarity1
Modified residuei229PhosphoserineBy similarity1
Modified residuei243PhosphoserineBy similarity1
Modified residuei266N6-acetyllysineCombined sources1
Modified residuei291PhosphoserineBy similarity1
Modified residuei297PhosphoserineBy similarity1
Modified residuei301PhosphoserineBy similarity1
Modified residuei502PhosphothreonineBy similarity1
Cross-linki582Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei669PhosphoserineBy similarity1
Cross-linki749Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei758PhosphoserineBy similarity1
Modified residuei779N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5HZG4
PaxDbiQ5HZG4
PeptideAtlasiQ5HZG4
PRIDEiQ5HZG4

PTM databases

iPTMnetiQ5HZG4
PhosphoSitePlusiQ5HZG4

Expressioni

Gene expression databases

BgeeiENSMUSG00000025782 Expressed in 254 organ(s), highest expression level in primary oocyte
ExpressionAtlasiQ5HZG4 baseline and differential
GenevisibleiQ5HZG4 MM

Interactioni

Subunit structurei

Belongs to the TFIID complex which is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TAF10 via histone fold. Interacts with TAF13, TBP, SAP130 and GCN5L2 (By similarity). Interacts with TBPL2.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Tbpl2Q6SJ953EBI-1561080,EBI-1571412

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229072, 2 interactors
ComplexPortaliCPX-932 General transcription factor complex TFIID
CPX-959 General transcription factor complex TFIID, Taf4b variant
IntActiQ5HZG4, 10 interactors
MINTiQ5HZG4
STRINGi10090.ENSMUSP00000026888

Structurei

Secondary structure

1932
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ5HZG4
SMRiQ5HZG4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5HZG4

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 141Poly-Glu6
Compositional biasi163 – 168Poly-Glu6
Compositional biasi221 – 367Pro-richAdd BLAST147
Compositional biasi508 – 749Lys-richAdd BLAST242
Compositional biasi782 – 834Pro-richAdd BLAST53

Domaini

The PHD-type zinc finger mediates binding to histone H3 methyllysine at position 4 (H3K4me3).

Sequence similaritiesi

Belongs to the TAF3 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri867 – 917PHD-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1973 Eukaryota
KOG2389 Eukaryota
ENOG410ZS4D LUCA
GeneTreeiENSGT00710000106806
HOGENOMiHOG000231905
HOVERGENiHBG083188
InParanoidiQ5HZG4
KOiK14650
OMAiKANMGTP
OrthoDBiEOG091G0XX7
PhylomeDBiQ5HZG4
TreeFamiTF316513

Family and domain databases

Gene3Di1.10.20.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR006565 BTP
IPR009072 Histone-fold
IPR037815 TAF3
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR12321:SF34 PTHR12321:SF34, 1 hit
PfamiView protein in Pfam
PF07524 Bromo_TP, 1 hit
PF00628 PHD, 1 hit
SMARTiView protein in SMART
SM00576 BTP, 1 hit
SM00249 PHD, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5HZG4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCESYSRSLL RVSVAQICQA LGWDSVQLSA CHLLTDVLQR YLQQLGRGCH
60 70 80 90 100
RYSELYGRTD PILDDVGEAF QLMGVNLHEL EDYIHNIEPV TFPHQIPSFP
110 120 130 140 150
VSKNNVLQFP QPGSKDAEER KDYIPDYLPP IVSSQEEEEE EQVPTDGGTS
160 170 180 190 200
AEAMQVPLEE DDEMEEEEVI NDENFLGKRP LDSPEVEEMP SMKRPRLLST
210 220 230 240 250
KGDSLDVVLL EAREPLSSIN PQKTPPVLSP VRVQDRADLA PPSPQPPMLA
260 270 280 290 300
PFAKSQLPIA KPLETKSFTP KTKTKASSPG QKTKSPKAAL SPARLGSPIR
310 320 330 340 350
SPKTIPKEKK SPGRSKSPKS PKSPKIVAHV PQTPVRPETP NRTPSAMVVE
360 370 380 390 400
KTVKETIPVM KPTQTPPEVV KLNIEMQPKK PVVTDKTIDD SIDAVIARAC
410 420 430 440 450
AEREPDPFEF SSGSESEGDT FTSPKRISGS ECATPKASTS SNNFTKSLAT
460 470 480 490 500
PLPLSSGTSS SDNSWTMDAS IDEVVRKAKL GAPSNMPPTF PYISSPSISP
510 520 530 540 550
PTPEPLHKGY EEKAKLPSSV DVKKKLKKEL KTKLKKKEKQ RDRERERERN
560 570 580 590 600
KERSKEKDKM REREKEKEAG KELKYPWREL MKDEDSDPYK FKIKEFEDID
610 620 630 640 650
AAKVRLKDGI VRREREKHKD KKKDRERSKR EKDKRERERL KEKNREDKIK
660 670 680 690 700
APPTQLVLPP KEMALPLFSP SAVRVPAMLP AFSPMLPEKL FEEKEKPKEK
710 720 730 740 750
ERKKDKKEKK KKKEKEKEKE KKEREREKER REREKREKEK EKHKHEKIKV
760 770 780 790 800
EPVIPAPSPV IPRLTLRVGA GQDKIVISKV VPAPEAKPAP SLNRPKTPPP
810 820 830 840 850
APVPIPVRVS PTPLQPPLLT QAAVCPALMP SPAPALSGIG SAKAPVRSVV
860 870 880 890 900
TETVSTYVIR DEWGNQIWIC PGCNKPDDGS PMIGCDDCDD WYHWPCVGIM
910 920 930
AAPPEEMQWF CPKCANKIKK DKKHKKRKHR AH
Length:932
Mass (Da):105,115
Last modified:July 11, 2006 - v2
Checksum:i8061D8E599A650EE
GO
Isoform 2 (identifier: Q5HZG4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-741: Missing.
     742-747: KHKHEK → MYKFPQ

Note: No experimental confirmation available.
Show »
Length:191
Mass (Da):20,794
Checksum:i55D0EC92119F1C03
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2ASY0A2ASY0_MOUSE
Transcription initiation factor TFI...
Taf3
779Annotation score:
A2ASX8A2ASX8_MOUSE
Transcription initiation factor TFI...
Taf3
51Annotation score:
A2ASX9A2ASX9_MOUSE
Transcription initiation factor TFI...
Taf3
108Annotation score:

Sequence cautioni

The sequence AAH89030 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti545R → G in BAE32543 (PubMed:16141072).Curated1
Sequence conflicti721K → E in BAE22792 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0197361 – 741Missing in isoform 2. 1 PublicationAdd BLAST741
Alternative sequenceiVSP_019737742 – 747KHKHEK → MYKFPQ in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292189 mRNA Translation: CAC34476.1
AK084574 mRNA Translation: BAC39218.1
AK136045 mRNA Translation: BAE22792.1
AK154372 mRNA Translation: BAE32543.1
BC089030 mRNA Translation: AAH89030.1 Different initiation.
CCDSiCCDS15675.1 [Q5HZG4-1]
RefSeqiNP_082024.2, NM_027748.3 [Q5HZG4-1]
UniGeneiMm.86343

Genome annotation databases

EnsembliENSMUST00000026888; ENSMUSP00000026888; ENSMUSG00000025782 [Q5HZG4-1]
GeneIDi209361
KEGGimmu:209361
UCSCiuc008ihk.2 mouse [Q5HZG4-2]
uc008ihl.2 mouse [Q5HZG4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292189 mRNA Translation: CAC34476.1
AK084574 mRNA Translation: BAC39218.1
AK136045 mRNA Translation: BAE22792.1
AK154372 mRNA Translation: BAE32543.1
BC089030 mRNA Translation: AAH89030.1 Different initiation.
CCDSiCCDS15675.1 [Q5HZG4-1]
RefSeqiNP_082024.2, NM_027748.3 [Q5HZG4-1]
UniGeneiMm.86343

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K16NMR-A857-924[»]
2K17NMR-A857-924[»]
ProteinModelPortaliQ5HZG4
SMRiQ5HZG4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229072, 2 interactors
ComplexPortaliCPX-932 General transcription factor complex TFIID
CPX-959 General transcription factor complex TFIID, Taf4b variant
IntActiQ5HZG4, 10 interactors
MINTiQ5HZG4
STRINGi10090.ENSMUSP00000026888

PTM databases

iPTMnetiQ5HZG4
PhosphoSitePlusiQ5HZG4

Proteomic databases

EPDiQ5HZG4
PaxDbiQ5HZG4
PeptideAtlasiQ5HZG4
PRIDEiQ5HZG4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026888; ENSMUSP00000026888; ENSMUSG00000025782 [Q5HZG4-1]
GeneIDi209361
KEGGimmu:209361
UCSCiuc008ihk.2 mouse [Q5HZG4-2]
uc008ihl.2 mouse [Q5HZG4-1]

Organism-specific databases

CTDi83860
MGIiMGI:2388097 Taf3

Phylogenomic databases

eggNOGiKOG1973 Eukaryota
KOG2389 Eukaryota
ENOG410ZS4D LUCA
GeneTreeiENSGT00710000106806
HOGENOMiHOG000231905
HOVERGENiHBG083188
InParanoidiQ5HZG4
KOiK14650
OMAiKANMGTP
OrthoDBiEOG091G0XX7
PhylomeDBiQ5HZG4
TreeFamiTF316513

Enzyme and pathway databases

ReactomeiR-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Miscellaneous databases

ChiTaRSiTaf3 mouse
EvolutionaryTraceiQ5HZG4
PROiPR:Q5HZG4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025782 Expressed in 254 organ(s), highest expression level in primary oocyte
ExpressionAtlasiQ5HZG4 baseline and differential
GenevisibleiQ5HZG4 MM

Family and domain databases

Gene3Di1.10.20.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR006565 BTP
IPR009072 Histone-fold
IPR037815 TAF3
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR12321:SF34 PTHR12321:SF34, 1 hit
PfamiView protein in Pfam
PF07524 Bromo_TP, 1 hit
PF00628 PHD, 1 hit
SMARTiView protein in SMART
SM00576 BTP, 1 hit
SM00249 PHD, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTAF3_MOUSE
AccessioniPrimary (citable) accession number: Q5HZG4
Secondary accession number(s): Q3U490
, Q3UWX2, Q8BIU8, Q99JH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: November 7, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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