UniProtKB - Q5H9I0 (TFDP3_HUMAN)
Protein
Transcription factor Dp family member 3
Gene
TFDP3
Organism
Homo sapiens (Human)
Status
Functioni
Competitive inhibitor of E2F-mediated transactivation activity. Impairs E2F-mediated cell-cycle progression from G1 to S phase.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 121 | Critical for repression of E2F activity | 1 | |
Sitei | 130 | Critical for repression of E2F activity | 1 | |
Sitei | 131 | Critical for repression of E2F activity | 1 | |
Sitei | 140 | Critical for repression of E2F activity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 108 – 190 | Sequence analysisAdd BLAST | 83 |
GO - Molecular functioni
- activating transcription factor binding Source: UniProtKB
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription factor activity Source: InterPro
- DNA-binding transcription factor inhibitor activity Source: ARUK-UCL
- transcription factor binding Source: UniProtKB
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB
- G1/S transition of mitotic cell cycle Source: InterPro
- negative regulation of DNA-binding transcription factor activity Source: UniProtKB
- negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q5H9I0 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor Dp family member 3Alternative name(s): Cancer/testis antigen 30 Short name: CT30 Hepatocellular carcinoma-associated antigen 661 |
Gene namesi | Name:TFDP3 Synonyms:DP4, HCA661 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24603, TFDP3 |
MIMi | 300772, gene |
neXtProti | NX_Q5H9I0 |
VEuPathDBi | HostDB:ENSG00000183434.9 |
Subcellular locationi
Nucleus
- nuclear chromatin Source: NTNU_SB
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
- transcription regulator complex Source: InterPro
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 112 | C → R: No effect on down-regulation of E2F transcriptional activity; when associated with or without R-161 or with V-164. | 1 | |
Mutagenesisi | 121 | T → K: Restores enhanced E2F-mediated transcriptional activity; when associated with Y-130; N-131 and E-145. 1 Publication | 1 | |
Mutagenesisi | 130 | C → Y: Restores enhanced E2F-mediated transcriptional activity; when associated with K-121; N-131 and E-145. 1 Publication | 1 | |
Mutagenesisi | 131 | Q → N: Restores enhanced E2F-mediated transcriptional activity; when associated with K-121; Y-130 and E-145. 1 Publication | 1 | |
Mutagenesisi | 140 | K → E: Restores enhanced E2F-mediated transcriptional activity; when associated with K-121; Y-130 and N-131. 1 Publication | 1 | |
Mutagenesisi | 161 | K → R: No effect on down-regulation of E2F transcriptional activity; when associated with or without R-112. | 1 | |
Mutagenesisi | 164 | T → V: No effect on down-regulation of E2F transcriptional activity; when associated R-112. | 1 |
Organism-specific databases
DisGeNETi | 51270 |
OpenTargetsi | ENSG00000183434 |
PharmGKBi | PA134898258 |
Miscellaneous databases
Pharosi | Q5H9I0, Tbio |
Genetic variation databases
BioMutai | TFDP3 |
DMDMi | 74762180 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000305940 | 1 – 405 | Transcription factor Dp family member 3Add BLAST | 405 |
Proteomic databases
jPOSTi | Q5H9I0 |
MassIVEi | Q5H9I0 |
PaxDbi | Q5H9I0 |
PeptideAtlasi | Q5H9I0 |
PRIDEi | Q5H9I0 |
ProteomicsDBi | 62889 |
PTM databases
iPTMneti | Q5H9I0 |
PhosphoSitePlusi | Q5H9I0 |
Expressioni
Tissue specificityi
Predominantly expressed in testis. Low level of expression in pancreas. Highly expressed in ovarian and colon cancer cell lines.2 Publications
Inductioni
In response to DNA damage.1 Publication
Gene expression databases
Bgeei | ENSG00000183434, Expressed in buccal mucosa cell and 14 other tissues |
Genevisiblei | Q5H9I0, HS |
Organism-specific databases
HPAi | ENSG00000183434, Tissue enriched (testis) |
Interactioni
Subunit structurei
Heterodimer: with E2F family members. TFDP3/E2F heterodimers do not bind DNA and repress E2F-dependent transcriptional activity.
2 PublicationsGO - Molecular functioni
- activating transcription factor binding Source: UniProtKB
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 119423, 30 interactors |
IntActi | Q5H9I0, 25 interactors |
STRINGi | 9606.ENSP00000385461 |
Miscellaneous databases
RNActi | Q5H9I0, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 175 – 306 | Involved in negatively regulating E2F activityAdd BLAST | 132 | |
Regioni | 209 – 241 | DCB1By similarityAdd BLAST | 33 | |
Regioni | 254 – 310 | DCB2By similarityAdd BLAST | 57 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 156 – 190 | DEF boxBy similarityAdd BLAST | 35 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 386 – 402 | Asp/Glu-rich (acidic; NCB domain)Add BLAST | 17 |
Domaini
The potential DNA-binding domain differs in sequence from that of other DP family members and cannot bind DNA.
Sequence similaritiesi
Belongs to the E2F/DP family.Curated
Phylogenomic databases
eggNOGi | KOG2829, Eukaryota |
GeneTreei | ENSGT00940000167066 |
HOGENOMi | CLU_039874_3_1_1 |
InParanoidi | Q5H9I0 |
OMAi | NIKRRTY |
OrthoDBi | 1046304at2759 |
PhylomeDBi | Q5H9I0 |
TreeFami | TF314396 |
Family and domain databases
CDDi | cd14458, DP_DD, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.20.140.80, 1 hit |
InterProi | View protein in InterPro IPR037241, E2F-DP_heterodim IPR003316, E2F_WHTH_DNA-bd_dom IPR038168, TF_DP_C_sf IPR028315, TFDP3 IPR014889, Transc_factor_DP_C IPR015648, Transcrpt_fac_DP IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12548, PTHR12548, 1 hit PTHR12548:SF13, PTHR12548:SF13, 1 hit |
Pfami | View protein in Pfam PF08781, DP, 1 hit PF02319, E2F_TDP, 1 hit |
PIRSFi | PIRSF009404, Transcription_factor_DP, 1 hit |
SMARTi | View protein in SMART SM01138, DP, 1 hit SM01372, E2F_TDP, 1 hit |
SUPFAMi | SSF144074, SSF144074, 1 hit SSF46785, SSF46785, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5H9I0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKYVSLTEA NEELKVLMDE NQTSRPVAVH TSTVNPLGKQ LLPKTFGQSS
60 70 80 90 100
VNIDQQVVIG MPQRPAASNI PVVGSPNPPS THFASQNQHS YSSPPWAGQH
110 120 130 140 150
NRKGEKNGMG LCRLSMKVWE TVQRKGTTSC QEVVGELVAK FRAASNHASP
160 170 180 190 200
NESAYDVKNI KRRTYDALNV LMAMNIISRE KKKIKWIGLT TNSAQNCQNL
210 220 230 240 250
RVERQKRLER IKQKQSELQQ LILQQIAFKN LVLRNQYVEE QVSQRPLPNS
260 270 280 290 300
VIHVPFIIIS SSKKTVINCS ISDDKSEYLF KFNSSFEIHD DTEVLMWMGM
310 320 330 340 350
TFGLESGSCS AEDLKMARNL VPKALEPYVT EMAQGTFGGV FTTAGSRSNG
360 370 380 390 400
TWLSASDLTN IAIGMLATSS GGSQYSGSRV ETPAVEEEEE EDNNDDDLSE
NDEDD
Sequence cautioni
The sequence AAF37562 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF219119 mRNA Translation: AAF37562.2 Different initiation. Z77249 Genomic DNA No translation available. CH471107 Genomic DNA Translation: EAX11773.1 |
CCDSi | CCDS14636.2 |
RefSeqi | NP_057605.3, NM_016521.2 |
Genome annotation databases
Ensembli | ENST00000310125; ENSP00000385461; ENSG00000183434 |
GeneIDi | 51270 |
KEGGi | hsa:51270 |
UCSCi | uc004exb.1, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF219119 mRNA Translation: AAF37562.2 Different initiation. Z77249 Genomic DNA No translation available. CH471107 Genomic DNA Translation: EAX11773.1 |
CCDSi | CCDS14636.2 |
RefSeqi | NP_057605.3, NM_016521.2 |
3D structure databases
SMRi | Q5H9I0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 119423, 30 interactors |
IntActi | Q5H9I0, 25 interactors |
STRINGi | 9606.ENSP00000385461 |
PTM databases
iPTMneti | Q5H9I0 |
PhosphoSitePlusi | Q5H9I0 |
Genetic variation databases
BioMutai | TFDP3 |
DMDMi | 74762180 |
Proteomic databases
jPOSTi | Q5H9I0 |
MassIVEi | Q5H9I0 |
PaxDbi | Q5H9I0 |
PeptideAtlasi | Q5H9I0 |
PRIDEi | Q5H9I0 |
ProteomicsDBi | 62889 |
Protocols and materials databases
Antibodypediai | 30265, 171 antibodies |
DNASUi | 51270 |
Genome annotation databases
Ensembli | ENST00000310125; ENSP00000385461; ENSG00000183434 |
GeneIDi | 51270 |
KEGGi | hsa:51270 |
UCSCi | uc004exb.1, human |
Organism-specific databases
CTDi | 51270 |
DisGeNETi | 51270 |
GeneCardsi | TFDP3 |
HGNCi | HGNC:24603, TFDP3 |
HPAi | ENSG00000183434, Tissue enriched (testis) |
MIMi | 300772, gene |
neXtProti | NX_Q5H9I0 |
OpenTargetsi | ENSG00000183434 |
PharmGKBi | PA134898258 |
VEuPathDBi | HostDB:ENSG00000183434.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2829, Eukaryota |
GeneTreei | ENSGT00940000167066 |
HOGENOMi | CLU_039874_3_1_1 |
InParanoidi | Q5H9I0 |
OMAi | NIKRRTY |
OrthoDBi | 1046304at2759 |
PhylomeDBi | Q5H9I0 |
TreeFami | TF314396 |
Enzyme and pathway databases
PathwayCommonsi | Q5H9I0 |
Miscellaneous databases
BioGRID-ORCSi | 51270, 0 hits in 528 CRISPR screens |
GenomeRNAii | 51270 |
Pharosi | Q5H9I0, Tbio |
PROi | PR:Q5H9I0 |
RNActi | Q5H9I0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000183434, Expressed in buccal mucosa cell and 14 other tissues |
Genevisiblei | Q5H9I0, HS |
Family and domain databases
CDDi | cd14458, DP_DD, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.20.140.80, 1 hit |
InterProi | View protein in InterPro IPR037241, E2F-DP_heterodim IPR003316, E2F_WHTH_DNA-bd_dom IPR038168, TF_DP_C_sf IPR028315, TFDP3 IPR014889, Transc_factor_DP_C IPR015648, Transcrpt_fac_DP IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12548, PTHR12548, 1 hit PTHR12548:SF13, PTHR12548:SF13, 1 hit |
Pfami | View protein in Pfam PF08781, DP, 1 hit PF02319, E2F_TDP, 1 hit |
PIRSFi | PIRSF009404, Transcription_factor_DP, 1 hit |
SMARTi | View protein in SMART SM01138, DP, 1 hit SM01372, E2F_TDP, 1 hit |
SUPFAMi | SSF144074, SSF144074, 1 hit SSF46785, SSF46785, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TFDP3_HUMAN | |
Accessioni | Q5H9I0Primary (citable) accession number: Q5H9I0 Secondary accession number(s): Q6DK49, Q9NZ54 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | February 15, 2005 | |
Last modified: | February 10, 2021 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families