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Protein

Serine/threonine-protein kinase TNNI3K

Gene

Tnni3k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May play a role in cardiac physiology.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei489ATPPROSITE-ProRule annotation1
Active sitei587Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi468 – 476ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase TNNI3K (EC:2.7.11.1)
Alternative name(s):
Cardiac ankyrin repeat kinase
TNNI3-interacting kinase
Gene namesi
Name:Tnni3kImported
Synonyms:Cark
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2443276 Tnni3k

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000867582 – 834Serine/threonine-protein kinase TNNI3KAdd BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ5GIG6
PRIDEiQ5GIG6

PTM databases

iPTMnetiQ5GIG6
PhosphoSitePlusiQ5GIG6

Expressioni

Gene expression databases

BgeeiENSMUSG00000040086 Expressed in 27 organ(s), highest expression level in heart
CleanExiMM_TNNI3K
GenevisibleiQ5GIG6 MM

Interactioni

Subunit structurei

Interacts with TNNI3, ACTC, ACTA1, MYBPC3, AIP, BABP3 and HADHB.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070561

Structurei

3D structure databases

ProteinModelPortaliQ5GIG6
SMRiQ5GIG6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati66 – 96ANK 1Add BLAST31
Repeati100 – 129ANK 2Add BLAST30
Repeati133 – 162ANK 3Add BLAST30
Repeati166 – 195ANK 4Add BLAST30
Repeati199 – 229ANK 5Add BLAST31
Repeati233 – 262ANK 6Add BLAST30
Repeati268 – 297ANK 7Add BLAST30
Repeati303 – 334ANK 8Add BLAST32
Repeati338 – 367ANK 9Add BLAST30
Repeati380 – 409ANK 10Add BLAST30
Domaini462 – 722Protein kinasePROSITE-ProRule annotationAdd BLAST261

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili21 – 49Sequence analysisAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi732 – 745Poly-SerAdd BLAST14

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IT5N Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150972
HOGENOMiHOG000234421
HOVERGENiHBG079513
InParanoidiQ5GIG6
KOiK17535
OMAiKGFFNIA
OrthoDBiEOG091G01QL
TreeFamiTF317710

Family and domain databases

CDDicd00204 ANK, 4 hits
Gene3Di1.25.40.20, 3 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF00023 Ank, 1 hit
PF12796 Ank_2, 3 hits
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 10 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 6 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q5GIG6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGNYKSRPTQ TCSDEWKKKV SESYAIIIER LEDDLQIKEN EFQELRHIFG
60 70 80 90 100
SDEAFSEVSL NYRTERGLSL LHLCCACGGN KSHIRALMLK GLRPSRLTRN
110 120 130 140 150
GFPALHLAVY KDSLELITSL LHSGADVQQA GYGGLTALHI AAIAGHPEAV
160 170 180 190 200
EVLLQHGANV NVQDAVFFTP LHIAAYYGHE QVTSVLLKFG ADVNVSGEVG
210 220 230 240 250
DRPLHLASAK GFFNIVKLLV EGNKADVNAQ DNEDHVPLHF CSRFGHHNIV
260 270 280 290 300
SYLLQSDLEV QPHVINIYGD TPLHLACYNG NFEVAKEIVH VTGTESLTKE
310 320 330 340 350
NIFSETAFHS ACTYGKNIDL VKFLLDQNAV NINHRGRDGH TGLHSACYHG
360 370 380 390 400
HIRLVQFLLD NGADMNLVAC DPSRSSGEKD EQTCLMWAYE KGHDAIVTLL
410 420 430 440 450
KHYKRPQDEL PCNEYSQPGG DGSYVSVPSP LGKIKSMTKE KADVLLLRAE
460 470 480 490 500
LPSRFHLQLS EIEFHEIIGS GSFGKVYKGR CRNKIVAIKR YRANTYCSKS
510 520 530 540 550
DVDMFCREVS ILCQLNHPCV VQFVGACLDD PSQFAIVTQY ISGGSLFSLL
560 570 580 590 600
HEQKRILDLQ SKLIIAVDVA KGMEYLHSLT QPIIHRDLNS HNILLYEDGH
610 620 630 640 650
AVVADFGESR FLQSLDEDNM TKQPGNLRWM APEVFTQCTR YTIKADVFSY
660 670 680 690 700
ALCLWELLTG EIPFAHLKPA AAAADMAYHH IRPPIGYSIP KPISSLLMRG
710 720 730 740 750
WNACPEGRPE FSEVVRKLEE CLCNVELMSP ASSNSSGSLS PSSSSDCLLS
760 770 780 790 800
RGGPGRSHVA ALRSRFELEY ALNARSYTGW PQSVGTHTNP GLSLEEMNRG
810 820 830
AQYSAVDKYG YVSDPMSPMH LHSRRNSGSF EDGN
Length:834
Mass (Da):92,575
Last modified:July 27, 2011 - v4
Checksum:iAC8581BFA7B05216
GO
Isoform 2 (identifier: Q5GIG6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     670-675: AAAAAD → GKTRVL
     676-834: Missing.

Show »
Length:675
Mass (Da):75,386
Checksum:i772B85A471A60D38
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti659T → I in AAS98608 (Ref. 1) Curated1
Sequence conflicti659T → I in AAS98609 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_051886670 – 675AAAAAD → GKTRVL in isoform 2. 1 Publication6
Alternative sequenceiVSP_051887676 – 834Missing in isoform 2. 1 PublicationAdd BLAST159

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY526095 mRNA Translation: AAS98608.1
AY526096 mRNA Translation: AAS98609.1
AC125097 Genomic DNA No translation available.
AC124174 Genomic DNA No translation available.
AC144762 Genomic DNA No translation available.
BC139368 mRNA Translation: AAI39369.1
BC139394 mRNA Translation: AAI39395.1
CCDSiCCDS17928.1 [Q5GIG6-1]
RefSeqiNP_796040.3, NM_177066.5 [Q5GIG6-1]
UniGeneiMm.210428

Genome annotation databases

EnsembliENSMUST00000064076; ENSMUSP00000070561; ENSMUSG00000040086 [Q5GIG6-1]
ENSMUST00000143410; ENSMUSP00000122478; ENSMUSG00000040086 [Q5GIG6-2]
GeneIDi435766
KEGGimmu:435766
UCSCiuc008rus.2 mouse [Q5GIG6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY526095 mRNA Translation: AAS98608.1
AY526096 mRNA Translation: AAS98609.1
AC125097 Genomic DNA No translation available.
AC124174 Genomic DNA No translation available.
AC144762 Genomic DNA No translation available.
BC139368 mRNA Translation: AAI39369.1
BC139394 mRNA Translation: AAI39395.1
CCDSiCCDS17928.1 [Q5GIG6-1]
RefSeqiNP_796040.3, NM_177066.5 [Q5GIG6-1]
UniGeneiMm.210428

3D structure databases

ProteinModelPortaliQ5GIG6
SMRiQ5GIG6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070561

PTM databases

iPTMnetiQ5GIG6
PhosphoSitePlusiQ5GIG6

Proteomic databases

PaxDbiQ5GIG6
PRIDEiQ5GIG6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064076; ENSMUSP00000070561; ENSMUSG00000040086 [Q5GIG6-1]
ENSMUST00000143410; ENSMUSP00000122478; ENSMUSG00000040086 [Q5GIG6-2]
GeneIDi435766
KEGGimmu:435766
UCSCiuc008rus.2 mouse [Q5GIG6-1]

Organism-specific databases

CTDi51086
MGIiMGI:2443276 Tnni3k

Phylogenomic databases

eggNOGiENOG410IT5N Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150972
HOGENOMiHOG000234421
HOVERGENiHBG079513
InParanoidiQ5GIG6
KOiK17535
OMAiKGFFNIA
OrthoDBiEOG091G01QL
TreeFamiTF317710

Miscellaneous databases

PROiPR:Q5GIG6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040086 Expressed in 27 organ(s), highest expression level in heart
CleanExiMM_TNNI3K
GenevisibleiQ5GIG6 MM

Family and domain databases

CDDicd00204 ANK, 4 hits
Gene3Di1.25.40.20, 3 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF00023 Ank, 1 hit
PF12796 Ank_2, 3 hits
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 10 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 6 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTNI3K_MOUSE
AccessioniPrimary (citable) accession number: Q5GIG6
Secondary accession number(s): B2RTJ7, Q5GIG5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 125 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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