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Protein

Arylsulfatase I

Gene

ARSI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts.2 Publications

Caution

According to PubMed:16500042, has no arylsulfatase activity.Curated

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55CalciumBy similarity1
Metal bindingi56CalciumBy similarity1
Active sitei93Nucleophile1 Publication1
Metal bindingi93Calcium; via 3-oxoalanineBy similarity1
Binding sitei147SubstrateBy similarity1
Active sitei149By similarity1
Binding sitei239SubstrateBy similarity1
Metal bindingi297CalciumBy similarity1
Metal bindingi298CalciumBy similarity1
Binding sitei315SubstrateBy similarity1

GO - Molecular functioni

  • arylsulfatase activity Source: HGNC
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1660662 Glycosphingolipid metabolism
R-HSA-1663150 The activation of arylsulfatases

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase I (EC:3.1.6.-)
Short name:
ASI
Gene namesi
Name:ARSI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000183876.8
HGNCiHGNC:32521 ARSI
MIMi610009 gene
neXtProtiNX_Q5FYB1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi93C → S: No arylsulfatase activity in the media of retinal epithelium cell. 1 Publication1

Organism-specific databases

DisGeNETi340075
MalaCardsiARSI
OpenTargetsiENSG00000183876
Orphaneti401815 Autosomal recessive spastic paraplegia type 66
PharmGKBiPA143485309

Polymorphism and mutation databases

BioMutaiARSI
DMDMi74722581

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000004221624 – 569Arylsulfatase IAdd BLAST546

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei933-oxoalanine (Cys)1 Publication1
Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi288N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi466N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi496N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The oxidation of Cys-93 residue to 3-oxoalanine (also known as C(alpha)-formylglycine) by SUMF1/Sulfatase-modifying factor 1, seems critical for catalytic activity.1 Publication

Keywords - PTMi

Glycoprotein, Oxidation

Proteomic databases

PaxDbiQ5FYB1
PeptideAtlasiQ5FYB1
PRIDEiQ5FYB1
ProteomicsDBi62821
62822 [Q5FYB1-2]

PTM databases

iPTMnetiQ5FYB1
PhosphoSitePlusiQ5FYB1

Expressioni

Tissue specificityi

Expressed in placenta, in embryonic stem cells, fetal eyes and lens.2 Publications

Gene expression databases

BgeeiENSG00000183876
CleanExiHS_ARSI
ExpressionAtlasiQ5FYB1 baseline and differential
GenevisibleiQ5FYB1 HS

Organism-specific databases

HPAiHPA038386
HPA038398

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT40Q6A1623EBI-10243706,EBI-10171697

Protein-protein interaction databases

BioGridi130992, 6 interactors
IntActiQ5FYB1, 1 interactor
STRINGi9606.ENSP00000333395

Structurei

3D structure databases

ProteinModelPortaliQ5FYB1
SMRiQ5FYB1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi527 – 533Poly-Glu7

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867 Eukaryota
COG3119 LUCA
GeneTreeiENSGT00760000119062
HOGENOMiHOG000135354
HOVERGENiHBG004282
InParanoidiQ5FYB1
KOiK12375
OMAiRYRSMGN
OrthoDBiEOG091G06C3
PhylomeDBiQ5FYB1
TreeFamiTF314186

Family and domain databases

Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR024607 Sulfatase_CS
IPR000917 Sulfatase_N
PfamiView protein in Pfam
PF00884 Sulfatase, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00523 SULFATASE_1, 1 hit
PS00149 SULFATASE_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5FYB1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHTLTGFSLV SLLSFGYLSW DWAKPSFVAD GPGEAGEQPS AAPPQPPHII
60 70 80 90 100
FILTDDQGYH DVGYHGSDIE TPTLDRLAAK GVKLENYYIQ PICTPSRSQL
110 120 130 140 150
LTGRYQIHTG LQHSIIRPQQ PNCLPLDQVT LPQKLQEAGY STHMVGKWHL
160 170 180 190 200
GFYRKECLPT RRGFDTFLGS LTGNVDYYTY DNCDGPGVCG FDLHEGENVA
210 220 230 240 250
WGLSGQYSTM LYAQRASHIL ASHSPQRPLF LYVAFQAVHT PLQSPREYLY
260 270 280 290 300
RYRTMGNVAR RKYAAMVTCM DEAVRNITWA LKRYGFYNNS VIIFSSDNGG
310 320 330 340 350
QTFSGGSNWP LRGRKGTYWE GGVRGLGFVH SPLLKRKQRT SRALMHITDW
360 370 380 390 400
YPTLVGLAGG TTSAADGLDG YDVWPAISEG RASPRTEILH NIDPLYNHAQ
410 420 430 440 450
HGSLEGGFGI WNTAVQAAIR VGEWKLLTGD PGYGDWIPPQ TLATFPGSWW
460 470 480 490 500
NLERMASVRQ AVWLFNISAD PYEREDLAGQ RPDVVRTLLA RLAEYNRTAI
510 520 530 540 550
PVRYPAENPR AHPDFNGGAW GPWASDEEEE EEEGRARSFS RGRRKKKCKI
560
CKLRSFFRKL NTRLMSQRI
Length:569
Mass (Da):64,030
Last modified:March 1, 2005 - v1
Checksum:iD2F33EDD33ED211C
GO
Isoform 2 (identifier: Q5FYB1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-143: Missing.

Show »
Length:426
Mass (Da):48,253
Checksum:i2A12FCB6D9DCFA32
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171L → F in BAG53634 (PubMed:14702039).Curated1
Sequence conflicti211L → P in BAG53634 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0360221 – 143Missing in isoform 2. 1 PublicationAdd BLAST143

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY875937 mRNA Translation: AAW66665.1
AB448735 mRNA Translation: BAH11166.1
AK122641 mRNA Translation: BAG53634.1
BC129995 mRNA Translation: AAI29996.1
BC129996 mRNA Translation: AAI29997.1
CCDSiCCDS34275.1 [Q5FYB1-1]
RefSeqiNP_001012301.1, NM_001012301.3 [Q5FYB1-1]
UniGeneiHs.591252

Genome annotation databases

EnsembliENST00000328668; ENSP00000333395; ENSG00000183876 [Q5FYB1-1]
ENST00000515301; ENSP00000426879; ENSG00000183876 [Q5FYB1-2]
GeneIDi340075
KEGGihsa:340075
UCSCiuc003lrv.3 human [Q5FYB1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARSI_HUMAN
AccessioniPrimary (citable) accession number: Q5FYB1
Secondary accession number(s): A1L3B0, B3KV22, B7XD03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: March 1, 2005
Last modified: June 20, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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