UniProtKB - Q5FX52 (PLCZ1_RAT)
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
Plcz1
Functioni
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
By similarityCurated1 PublicationCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H+By similarityEC:3.1.4.11By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 177 | PROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 222 | PROSITE-ProRule annotationBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phosphatidylinositol-3-phosphate binding Source: RGD
- phosphatidylinositol-4,5-bisphosphate binding Source: RGD
- phosphatidylinositol-5-phosphate binding Source: RGD
- phosphatidylinositol phospholipase C activity Source: RGD
- phospholipase C activity Source: RGD
GO - Biological processi
- calcium ion transport Source: RGD
- egg activation Source: UniProtKB
- lipid catabolic process Source: UniProtKB-KW
- phosphatidylinositol-mediated signaling Source: GO_Central
- positive regulation of cytosolic calcium ion concentration Source: RGD
- positive regulation of cytosolic calcium ion concentration involved in egg activation Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Hydrolase, Transducer |
Biological process | Fertilization, Lipid degradation, Lipid metabolism |
Ligand | Calcium |
Enzyme and pathway databases
Reactomei | R-RNO-1855204, Synthesis of IP3 and IP4 in the cytosol |
Names & Taxonomyi
Protein namesi | Recommended name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)Alternative name(s): Phosphoinositide phospholipase C-zeta-1 Phospholipase C-zeta-1By similarity Short name: PLC-zeta-1By similarity |
Gene namesi | Name:Plcz1Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1359567, Plcz1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- perinuclear region By similarity
Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis (By similarity).By similarity
Cytosol
- cytosol Source: InterPro
Nucleus
- nucleolus Source: RGD
- nucleoplasm Source: RGD
- nucleus Source: RGD
- pronucleus Source: RGD
Other locations
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- sperm head Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000347248 | 1 – 645 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1Add BLAST | 645 |
Proteomic databases
PaxDbi | Q5FX52 |
PTM databases
iPTMneti | Q5FX52 |
PhosphoSitePlusi | Q5FX52 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000008514, Expressed in testis and 3 other tissues |
Interactioni
Subunit structurei
Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000011376 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 42 – 77 | EF-handPROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 162 – 306 | PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST | 145 | |
Domaini | 385 – 501 | PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST | 117 | |
Domaini | 501 – 625 | C2PROSITE-ProRule annotationAdd BLAST | 125 |
Domaini
Phylogenomic databases
eggNOGi | KOG0169, Eukaryota |
GeneTreei | ENSGT00940000159950 |
HOGENOMi | CLU_002738_0_3_1 |
InParanoidi | Q5FX52 |
OMAi | YLTCTLV |
OrthoDBi | 368239at2759 |
PhylomeDBi | Q5FX52 |
TreeFami | TF313216 |
Family and domain databases
Gene3Di | 2.60.40.150, 1 hit 3.20.20.190, 1 hit |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR011992, EF-hand-dom_pair IPR002048, EF_hand_dom IPR001192, PI-PLC_fam IPR017946, PLC-like_Pdiesterase_TIM-brl IPR028395, PLC-zeta1 IPR015359, PLC_EF-hand-like IPR000909, PLipase_C_PInositol-sp_X_dom IPR001711, PLipase_C_Pinositol-sp_Y |
PANTHERi | PTHR10336, PTHR10336, 1 hit PTHR10336:SF29, PTHR10336:SF29, 1 hit |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF09279, EF-hand_like, 1 hit PF00388, PI-PLC-X, 1 hit PF00387, PI-PLC-Y, 1 hit |
PRINTSi | PR00390, PHPHLIPASEC |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00148, PLCXc, 1 hit SM00149, PLCYc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49562, SSF49562, 1 hit SSF51695, SSF51695, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS50222, EF_HAND_2, 1 hit PS50007, PIPLC_X_DOMAIN, 1 hit PS50008, PIPLC_Y_DOMAIN, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MESHYELAEA RWFMSKIQDY FRGGKISAGI THKLLEKLDF PCHFAHVKRI
60 70 80 90 100
FKENDRHNQG RITTEDFRTI YRCIVHREEI VEIFNTYTEN RKILPEDSLI
110 120 130 140 150
EFLTQEQYEM EMDESSSVEI IQKYEPIAEV KNERQMSIEG FARYMFSSEC
160 170 180 190 200
LLFKETCNTV YQDMNKPLND YYISSSHNTY LISDQILGPS DIWGYISALV
210 220 230 240 250
KGCRCLEIDC WDGAQNEPIV YHGYTLTSKL LFKTVIQAIN KYAFVTSDYP
260 270 280 290 300
VVLSLENHCS PGQQEVMTDI LQSTFGDFLL SDILDEFPDS LPSPEALKFK
310 320 330 340 350
ILVKNKKVGT LSETRERLGT DKRGIALDLE EEIYENEDED SGKEPETWDD
360 370 380 390 400
FLSRVKEEQE ADPSTLSGIA DAKKKIRKLR VALALSDLVI YTKAEKFRNF
410 420 430 440 450
QYSRVYQQFN ETTSMGESRA RKLSKLRAHE FIFHTAAFIT RVYPKFTRAD
460 470 480 490 500
SSNFNPQEFW NVGCQMVALN FQTPGLPMDL QNGKFLDNGG SGYVLKPDFL
510 520 530 540 550
RDTTLGFNPN EPEGDGHPVT LTIRLISGIQ LPVNVPSNTS DIIVIIEVYG
560 570 580 590 600
VPNDHMKQQS RAVKNNAFSP RWNETFTFLI QVPELALIRF VVETQGFLSG
610 620 630 640
NELLGQYTLP VLCMNKGYRR VPLFSKSGAN LEPSSLFIYV WYYRE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY885259 mRNA Translation: AAW66659.1 |
RefSeqi | NP_001012234.1, NM_001012234.1 XP_006237659.1, XM_006237597.3 XP_017448259.1, XM_017592770.1 |
Genome annotation databases
Ensembli | ENSRNOT00000011376; ENSRNOP00000011376; ENSRNOG00000008514 |
GeneIDi | 497197 |
KEGGi | rno:497197 |
UCSCi | RGD:1359567, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY885259 mRNA Translation: AAW66659.1 |
RefSeqi | NP_001012234.1, NM_001012234.1 XP_006237659.1, XM_006237597.3 XP_017448259.1, XM_017592770.1 |
3D structure databases
SMRi | Q5FX52 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000011376 |
PTM databases
iPTMneti | Q5FX52 |
PhosphoSitePlusi | Q5FX52 |
Proteomic databases
PaxDbi | Q5FX52 |
Genome annotation databases
Ensembli | ENSRNOT00000011376; ENSRNOP00000011376; ENSRNOG00000008514 |
GeneIDi | 497197 |
KEGGi | rno:497197 |
UCSCi | RGD:1359567, rat |
Organism-specific databases
CTDi | 89869 |
RGDi | 1359567, Plcz1 |
Phylogenomic databases
eggNOGi | KOG0169, Eukaryota |
GeneTreei | ENSGT00940000159950 |
HOGENOMi | CLU_002738_0_3_1 |
InParanoidi | Q5FX52 |
OMAi | YLTCTLV |
OrthoDBi | 368239at2759 |
PhylomeDBi | Q5FX52 |
TreeFami | TF313216 |
Enzyme and pathway databases
Reactomei | R-RNO-1855204, Synthesis of IP3 and IP4 in the cytosol |
Miscellaneous databases
PROi | PR:Q5FX52 |
Gene expression databases
Bgeei | ENSRNOG00000008514, Expressed in testis and 3 other tissues |
Family and domain databases
Gene3Di | 2.60.40.150, 1 hit 3.20.20.190, 1 hit |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR011992, EF-hand-dom_pair IPR002048, EF_hand_dom IPR001192, PI-PLC_fam IPR017946, PLC-like_Pdiesterase_TIM-brl IPR028395, PLC-zeta1 IPR015359, PLC_EF-hand-like IPR000909, PLipase_C_PInositol-sp_X_dom IPR001711, PLipase_C_Pinositol-sp_Y |
PANTHERi | PTHR10336, PTHR10336, 1 hit PTHR10336:SF29, PTHR10336:SF29, 1 hit |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF09279, EF-hand_like, 1 hit PF00388, PI-PLC-X, 1 hit PF00387, PI-PLC-Y, 1 hit |
PRINTSi | PR00390, PHPHLIPASEC |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00148, PLCXc, 1 hit SM00149, PLCYc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49562, SSF49562, 1 hit SSF51695, SSF51695, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS50222, EF_HAND_2, 1 hit PS50007, PIPLC_X_DOMAIN, 1 hit PS50008, PIPLC_Y_DOMAIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PLCZ1_RAT | |
Accessioni | Q5FX52Primary (citable) accession number: Q5FX52 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | March 1, 2005 | |
Last modified: | February 23, 2022 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |