UniProtKB - Q5FWY4 (MCM6M_XENLA)
Maternal DNA replication licensing factor mcm6
mmcm6
Functioni
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 160 – 187 | C4-typeSequence analysisAdd BLAST | 28 | |
Nucleotide bindingi | 398 – 405 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- DNA replication initiation Source: InterPro
- DNA unwinding involved in DNA replication Source: UniProtKB
- regulation of DNA-dependent DNA replication initiation Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Maternal DNA replication licensing factor mcm6 (EC:3.6.4.12)Alternative name(s): Maternal minichromosome maintenance protein 6 Short name: mMCM6 Short name: xMCM6 |
Gene namesi | Name:mmcm61 Publication |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-5870555, mcm6.L |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Other locations
- Chromosome 2 Publications
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.2 Publications
Nucleus
- CMG complex Source: UniProtKB
Other locations
- chromatin Source: UniProtKB
- MCM complex Source: UniProtKB
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 404 | K → A: Reduces ATPase activity of the mcm2-mcm7 complex by 50% and disrupts DNA replication. Does not disrupt formation of the pre-replicative complex onto chromatin. Abolishes ATPase activity of the mcm2-mcm7 complex; when associated with A-386 in mcm7. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000235887 | 1 – 821 | Maternal DNA replication licensing factor mcm6Add BLAST | 821 |
Proteomic databases
MaxQBi | Q5FWY4 |
Expressioni
Developmental stagei
Interactioni
Subunit structurei
Component of the mcm2-7 complex (RLF-M) (PubMed:16369567, PubMed:9214646, PubMed:9214647, PubMed:9851868). The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (PubMed:16369567, PubMed:9214646, PubMed:9214647, PubMed:9851868). The heterodimer of mmcm3/mcm5 interacts with mcm4, mmcm6, mcm7 and weakly with mcm2 (PubMed:16369567, PubMed:9214646, PubMed:9214647, PubMed:9851868).
Component of the CMG helicase complex, composed of the mcm2-7 complex, the GINS complex and cdc45 (PubMed:30979826, PubMed:30842657).
6 PublicationsProtein-protein interaction databases
BioGRIDi | 99406, 1 interactor |
IntActi | Q5FWY4, 2 interactors |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 348 – 555 | MCMSequence analysisAdd BLAST | 208 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 530 – 533 | Arginine finger | 4 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 160 – 187 | C4-typeSequence analysisAdd BLAST | 28 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
OMAi | VQDENMA |
OrthoDBi | 266497at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008049, MCM6 IPR041024, Mcm6_C IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF18263, MCM6_C, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01662, MCMPROTEIN6 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MELGGPAAAG DTDIAGQQLF KDELSDKCQK LFLEFLEECK GKDGSNLYVS
60 70 80 90 100
AAEELIRPER NTLAVNFTDI EYYNQQLATT IQEEYYRVYP HLCRAVRSFA
110 120 130 140 150
RQMGNIPANK EFYIAFSDFP ARQKIRELSS AKIGTLLRIS GQVVRTHPVH
160 170 180 190 200
PELVSGTFLC MDCQSIVKDV EQQFRYTQPT ICKNPVCANR RRFTLDTNKS
210 220 230 240 250
RFVDFQKVRI QETQAELPRG AIPRSVEIIL RAEAVESAMA GDRCDFTGTL
260 270 280 290 300
IVVPDVSALA AGDARMETGA KVTGGEGFNS EGVQGLKALG VRDLSYRLAF
310 320 330 340 350
LACYVGATNP RFGGKDLREE DQTAESIKNQ MTVQEWEKVF EMSQDKNLYH
360 370 380 390 400
NLCTSLFPTI HGNDEIKRGV LLMLFGGVPK TTMEGTSLRG DINVCIVGDP
410 420 430 440 450
STSKSQFLKH VEEFSPRAVY TSGKASSAAG LTAAVVKDEE SHEFVIEAGA
460 470 480 490 500
LMLADNGVCC IDEFDKMDLK DQVAIHEAME QQTISITKAG VKATLNARTS
510 520 530 540 550
ILAAANPVGG RYERSKSLKH NVNLSAPIMS RFDLFFILVD ECNEVTDYAI
560 570 580 590 600
ARRIVDLHAR NEESIERVYS IEDIQRYLLF ARQFQPKITK EAEEFIVEQY
610 620 630 640 650
RRLRQRDGSG VAKSSWRITV RQLESLIRLS ESMARMHCSD EVQPKHVKEA
660 670 680 690 700
FRLLSKSIIR VDTPDVSFDQ GEDEKNIEGE NNGNLNNGEE AMETNQDEPI
710 720 730 740 750
NEKPSSNAGL KMSFAEYKQI SNLLVLYMQK MEETEEECHL TTTDLVNWYL
760 770 780 790 800
KEMEAEIETE TELILKKRLI EKVIHRLIYY DHILIELNKS ELKTMDDTKE
810 820
TGEDAAEDRI LVVNPNYMLE D
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 | L → F in AAC60226 (PubMed:9214647).Curated | 1 | |
Sequence conflicti | 259 | L → F in AAC60226 (PubMed:9214647).Curated | 1 | |
Sequence conflicti | 786 – 821 | ELNKS…YMLED → RTEQIGIKNNG in AAC60226 (PubMed:9214647).CuratedAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U44050 mRNA Translation: AAC60226.1 BC089118 mRNA Translation: AAH89118.1 |
PIRi | T47222 |
RefSeqi | NP_001081822.1, NM_001088353.1 |
Genome annotation databases
GeneIDi | 398071 |
KEGGi | xla:398071 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U44050 mRNA Translation: AAC60226.1 BC089118 mRNA Translation: AAH89118.1 |
PIRi | T47222 |
RefSeqi | NP_001081822.1, NM_001088353.1 |
3D structure databases
SMRi | Q5FWY4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 99406, 1 interactor |
IntActi | Q5FWY4, 2 interactors |
Proteomic databases
MaxQBi | Q5FWY4 |
Genome annotation databases
GeneIDi | 398071 |
KEGGi | xla:398071 |
Organism-specific databases
CTDi | 398071 |
Xenbasei | XB-GENE-5870555, mcm6.L |
Phylogenomic databases
OMAi | VQDENMA |
OrthoDBi | 266497at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR008049, MCM6 IPR041024, Mcm6_C IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF18263, MCM6_C, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01662, MCMPROTEIN6 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM6M_XENLA | |
Accessioni | Q5FWY4Primary (citable) accession number: Q5FWY4 Secondary accession number(s): O42590 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | March 1, 2005 | |
Last modified: | December 2, 2020 | |
This is version 85 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |