UniProtKB - Q5FVP8 (DGAT2_RAT)
Protein
Diacylglycerol O-acyltransferase 2
Gene
Dgat2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides (By similarity). Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (By similarity).By similarity
Catalytic activityi
- EC:2.3.1.20By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.3.1.76By similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O-(9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1,3-di-(9Z-octadecenoyl)-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 2,3-di-(9Z)-octadecenoyl-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- 2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Inhibited by niacin.By similarity
: triacylglycerol biosynthesis Pathwayi
This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.
GO - Molecular functioni
- 2-acylglycerol O-acyltransferase activity Source: RGD
- diacylglycerol O-acyltransferase activity Source: RGD
- O-acyltransferase activity Source: GO_Central
- protein homodimerization activity Source: RGD
- retinol O-fatty-acyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- bile acid signaling pathway Source: RGD
- cellular response to oleic acid Source: RGD
- cellular triglyceride homeostasis Source: RGD
- cholesterol homeostasis Source: RGD
- diacylglycerol biosynthetic process Source: UniProtKB
- diacylglycerol metabolic process Source: RGD
- fat pad development Source: RGD
- fatty acid homeostasis Source: RGD
- glycerol metabolic process Source: UniProtKB-KW
- lipid metabolic process Source: GO_Central
- lipid storage Source: RGD
- long-chain fatty-acyl-CoA metabolic process Source: RGD
- low-density lipoprotein particle clearance Source: RGD
- monoacylglycerol biosynthetic process Source: UniProtKB
- negative regulation of fatty acid oxidation Source: RGD
- positive regulation of gluconeogenesis Source: RGD
- positive regulation of triglyceride biosynthetic process Source: RGD
- regulation of cholesterol metabolic process Source: RGD
- regulation of lipoprotein metabolic process Source: RGD
- regulation of plasma lipoprotein particle levels Source: RGD
- triglyceride biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Glycerol metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-RNO-1482883, Acyl chain remodeling of DAG and TAG R-RNO-75109, Triglyceride biosynthesis |
UniPathwayi | UPA00282 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Dgat2Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620329, Dgat2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Other locations
- Lipid droplet By similarity
- perinuclear region By similarity
Cytosol
- cytosol Source: Ensembl
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
- endoplasmic reticulum membrane Source: UniProtKB
- integral component of endoplasmic reticulum membrane Source: RGD
- perinuclear endoplasmic reticulum membrane Source: UniProtKB
Mitochondrion
- mitochondrion Source: Ensembl
Other locations
- integral component of membrane Source: RGD
- intracellular membrane-bounded organelle Source: RGD
- lipid droplet Source: UniProtKB
- membrane Source: RGD
- perinuclear region of cytoplasm Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 69 | CytoplasmicSequence analysisAdd BLAST | 69 | |
Transmembranei | 70 – 88 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 89 – 92 | LumenalSequence analysis | 4 | |
Transmembranei | 93 – 112 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 113 – 388 | CytoplasmicSequence analysisAdd BLAST | 276 |
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Lipid droplet, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249047 | 1 – 388 | Diacylglycerol O-acyltransferase 2Add BLAST | 388 |
Proteomic databases
PaxDbi | Q5FVP8 |
PRIDEi | Q5FVP8 |
PTM databases
PhosphoSitePlusi | Q5FVP8 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000016573, Expressed in liver and 21 other tissues |
ExpressionAtlasi | Q5FVP8, baseline and differential |
Genevisiblei | Q5FVP8, RN |
Interactioni
Subunit structurei
Forms multimeric complexes consisting of several DGAT2 subunits (By similarity).
Interacts with SLC27A1 and this interaction is enhanced in the presence of ZFYVE1 (By similarity).
By similarityGO - Molecular functioni
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000022557 |
Family & Domainsi
Sequence similaritiesi
Belongs to the diacylglycerol acyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT01010000222418 |
HOGENOMi | CLU_023995_0_1_1 |
InParanoidi | Q5FVP8 |
OMAi | LIYMFCT |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q5FVP8 |
TreeFami | TF314707 |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q5FVP8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTLIAAYSG VLRGERRAEA ARSENKNKGS ALSREGSGRW GTGSSILSAL
60 70 80 90 100
QDIFSVTWLN RSKVEKHLQV ISVLQWVLSF LVLGVACSVI LMYTFCTDCW
110 120 130 140 150
LIAALYFTWL AFDWNTPKKG GRRSQWVRNW AVWRYFRDYF PIQLVKTHNL
160 170 180 190 200
LTTRNYIFGY HPHGIMGLGA FCNFSTEATE VSKKFPGIRP YLATLAGNFR
210 220 230 240 250
MPVLREYLMS GGICPVNRDT IDYLLSKNGS GNAIVIVVGG AAESLSSMPG
260 270 280 290 300
KNAVTLRNRK GFVKLALRHG ADLVPTYSFG ENEVYKQVIF EEGSWGRWVQ
310 320 330 340 350
KKFQKYIGFA PCIFHGRGLF SSDTWGLVPY SKPITTVVGE PITVPKLEHP
360 370 380
TQKDIDLYHT MYMEALVKLF DNHKTKFGLP ETEVLEVN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JUZ8 | A0A0G2JUZ8_RAT | Acyltransferase | Dgat2 | 358 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC089846 mRNA Translation: AAH89846.1 AJ487787 mRNA Translation: CAD32178.1 |
RefSeqi | NP_001012345.1, NM_001012345.1 |
Genome annotation databases
Ensembli | ENSRNOT00000022557; ENSRNOP00000022557; ENSRNOG00000016573 |
GeneIDi | 252900 |
KEGGi | rno:252900 |
UCSCi | RGD:620329, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC089846 mRNA Translation: AAH89846.1 AJ487787 mRNA Translation: CAD32178.1 |
RefSeqi | NP_001012345.1, NM_001012345.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000022557 |
Chemistry databases
ChEMBLi | CHEMBL4295847 |
PTM databases
PhosphoSitePlusi | Q5FVP8 |
Proteomic databases
PaxDbi | Q5FVP8 |
PRIDEi | Q5FVP8 |
Genome annotation databases
Ensembli | ENSRNOT00000022557; ENSRNOP00000022557; ENSRNOG00000016573 |
GeneIDi | 252900 |
KEGGi | rno:252900 |
UCSCi | RGD:620329, rat |
Organism-specific databases
CTDi | 84649 |
RGDi | 620329, Dgat2 |
Phylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT01010000222418 |
HOGENOMi | CLU_023995_0_1_1 |
InParanoidi | Q5FVP8 |
OMAi | LIYMFCT |
OrthoDBi | 1347007at2759 |
PhylomeDBi | Q5FVP8 |
TreeFami | TF314707 |
Enzyme and pathway databases
UniPathwayi | UPA00282 |
Reactomei | R-RNO-1482883, Acyl chain remodeling of DAG and TAG R-RNO-75109, Triglyceride biosynthesis |
Miscellaneous databases
PROi | PR:Q5FVP8 |
Gene expression databases
Bgeei | ENSRNOG00000016573, Expressed in liver and 21 other tissues |
ExpressionAtlasi | Q5FVP8, baseline and differential |
Genevisiblei | Q5FVP8, RN |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DGAT2_RAT | |
Accessioni | Q5FVP8Primary (citable) accession number: Q5FVP8 Secondary accession number(s): Q8K4Y4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | March 1, 2005 | |
Last modified: | April 7, 2021 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families