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Protein

Protein-glutamate methylesterase/protein-glutamine glutaminase

Gene

cheB

Organism
Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.UniRule annotation

Catalytic activityi

Protein L-glutamate O5-methyl ester + H2O = protein L-glutamate + methanol.UniRule annotation
Protein L-glutamine + H2O = protein L-glutamate + NH3.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei162UniRule annotation1
Active sitei188UniRule annotation1
Active sitei287UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processChemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamate methylesterase/protein-glutamine glutaminaseUniRule annotation (EC:3.1.1.61UniRule annotation, EC:3.5.1.44UniRule annotation)
Gene namesi
Name:cheBUniRule annotation
Ordered Locus Names:GOX1555
OrganismiGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Taxonomic identifieri290633 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconobacter
Proteomesi
  • UP000006375 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002254621 – 351Protein-glutamate methylesterase/protein-glutamine glutaminaseAdd BLAST351

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei594-aspartylphosphateUniRule annotation1

Post-translational modificationi

Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi290633.GOX1555

Structurei

3D structure databases

ProteinModelPortaliQ5FQQ0
SMRiQ5FQQ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 125Response regulatoryUniRule annotationAdd BLAST118
Domaini151 – 345CheB-type methylesteraseUniRule annotationAdd BLAST195

Domaini

Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.UniRule annotation

Sequence similaritiesi

Belongs to the CheB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CMP Bacteria
COG2201 LUCA
HOGENOMiHOG000151423
KOiK03412
OMAiAMVKIRQ
OrthoDBiPOG091H045J

Family and domain databases

CDDicd16432 CheB_Rec, 1 hit
cd00156 REC, 1 hit
Gene3Di3.40.50.180, 1 hit
HAMAPiMF_00099 CheB_chemtxs, 1 hit
InterProiView protein in InterPro
IPR035909 CheB_C
IPR011006 CheY-like_superfamily
IPR008248 Sig_transdc_resp-reg_CheB
IPR000673 Sig_transdc_resp-reg_Me-estase
IPR001789 Sig_transdc_resp-reg_receiver
PfamiView protein in Pfam
PF01339 CheB_methylest, 1 hit
PF00072 Response_reg, 1 hit
PIRSFiPIRSF000876 RR_chemtxs_CheB, 1 hit
SMARTiView protein in SMART
SM00448 REC, 1 hit
SUPFAMiSSF52172 SSF52172, 1 hit
SSF52738 SSF52738, 1 hit
PROSITEiView protein in PROSITE
PS50122 CHEB, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

Q5FQQ0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGARPLTVV VVDDSLTTRA LIAAALRRDP RVKIVGTAGT PFEARDLIVR
60 70 80 90 100
LNPDVLTLDF EMPSMNGLQF LEKIMRLRPM PVVMVSGHLS SHRGLVEQSL
110 120 130 140 150
RMGAAEWYSK TTTANGEDPF AGLGDAIVRA AAMKQQAPVP VATDSANRTV
160 170 180 190 200
PKIGTVVGIG ASTGGVEALT DVLGHFPVAC PPTVIVQHMP RQFSESFARR
210 220 230 240 250
LDRIVKPEVR LAEEGDMLKP GTVYVASGGE RHLRVRCGAR SGEYVCQLVA
260 270 280 290 300
APPVNGHRPS VDELFYSLAT TVGKNAVGVI LTGMECDGAQ GLLAMRHAGA
310 320 330 340 350
STIGQDRASS VIYGMPRAAY ELGAVETQLP LSAIGPAILN LCERRSLETY

P
Length:351
Mass (Da):37,388
Last modified:March 1, 2005 - v1
Checksum:iB6F76C0DD3EAFB0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000009 Genomic DNA Translation: AAW61296.1
RefSeqiWP_011253081.1, NZ_LT900338.1

Genome annotation databases

EnsemblBacteriaiAAW61296; AAW61296; GOX1555
GeneIDi29629138
KEGGigox:GOX1555

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000009 Genomic DNA Translation: AAW61296.1
RefSeqiWP_011253081.1, NZ_LT900338.1

3D structure databases

ProteinModelPortaliQ5FQQ0
SMRiQ5FQQ0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290633.GOX1555

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW61296; AAW61296; GOX1555
GeneIDi29629138
KEGGigox:GOX1555

Phylogenomic databases

eggNOGiENOG4105CMP Bacteria
COG2201 LUCA
HOGENOMiHOG000151423
KOiK03412
OMAiAMVKIRQ
OrthoDBiPOG091H045J

Family and domain databases

CDDicd16432 CheB_Rec, 1 hit
cd00156 REC, 1 hit
Gene3Di3.40.50.180, 1 hit
HAMAPiMF_00099 CheB_chemtxs, 1 hit
InterProiView protein in InterPro
IPR035909 CheB_C
IPR011006 CheY-like_superfamily
IPR008248 Sig_transdc_resp-reg_CheB
IPR000673 Sig_transdc_resp-reg_Me-estase
IPR001789 Sig_transdc_resp-reg_receiver
PfamiView protein in Pfam
PF01339 CheB_methylest, 1 hit
PF00072 Response_reg, 1 hit
PIRSFiPIRSF000876 RR_chemtxs_CheB, 1 hit
SMARTiView protein in SMART
SM00448 REC, 1 hit
SUPFAMiSSF52172 SSF52172, 1 hit
SSF52738 SSF52738, 1 hit
PROSITEiView protein in PROSITE
PS50122 CHEB, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHEB_GLUOX
AccessioniPrimary (citable) accession number: Q5FQQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 1, 2005
Last modified: November 7, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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