UniProtKB - Q5FBB7 (SGO1_HUMAN)
Shugoshin 1
SGO1
Functioni
Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase.
8 PublicationsMiscellaneous
GO - Molecular functioni
- kinase binding Source: MGI
GO - Biological processi
- attachment of spindle microtubules to kinetochore Source: UniProtKB
- cell division Source: UniProtKB-KW
- centriole-centriole cohesion Source: UniProtKB
- chromosome segregation Source: UniProtKB
- homologous chromosome segregation Source: GO_Central
- meiotic chromosome segregation Source: MGI
- meiotic sister chromatid cohesion Source: GO_Central
- mitotic sister chromatid cohesion, centromeric Source: UniProtKB
- mitotic sister chromatid segregation Source: GO_Central
Keywordsi
Biological process | Cell cycle, Cell division, Chromosome partition, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q5FBB7 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q5FBB7 |
SIGNORi | Q5FBB7 |
Names & Taxonomyi
Protein namesi | Recommended name: Shugoshin 1ImportedAlternative name(s): Serologically defined breast cancer antigen NY-BR-85 Shugoshin-like 1 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25088, SGO1 |
MIMi | 609168, gene |
neXtProti | NX_Q5FBB7 |
VEuPathDBi | HostDB:ENSG00000129810 |
Subcellular locationi
Cytoskeleton
- spindle pole 2 Publications
- centrosome 1 Publication
Nucleus
- Nucleus 1 Publication
Other locations
- centromere 5 Publications
- kinetochore 3 Publications
Note: Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase (PubMed:16541025). Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A (PubMed:16580887)(PubMed:16541025)(PubMed:15604152). Colocalizes with NEK2 at the kinetochore (PubMed:17621308). Colocalizes with and SS18L1 at the kinetochore (PubMed:16582621). Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore (PubMed:17617734). Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis (PubMed:16582621). Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent (PubMed:16582621). Isoform 3 does not localize to kinetochores during any stages of the cell cycle (PubMed:16582621).6 Publications
Cytoskeleton
- centrosome Source: UniProtKB
- spindle pole Source: UniProtKB
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
Other locations
- chromosome, centromeric region Source: UniProtKB
- condensed chromosome, centromeric region Source: UniProtKB
- kinetochore Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, NucleusPathology & Biotechi
Involvement in diseasei
Chronic atrial and intestinal dysrhythmia (CAID)1 Publication
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_072709 | 23 | K → E in CAID; patient fibroblasts exhibit significantly faster cell proliferation than controls; during mitosis the mutant protein is localized in an ordered fashion around the centromeres but display a rather homogeneous cytoplasmic localization pattern. 1 PublicationCorresponds to variant dbSNP:rs199815268EnsemblClinVar. | 1 |
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 14 | S → A: Loss of phosphorylation and presence of misaligned chromosomes; when associated with A-507. 1 Publication | 1 | |
Mutagenesisi | 61 | N → I: Loss of interaction with PPP2CA and PPP2R1A and loss of centromeric localization. 1 Publication | 1 | |
Mutagenesisi | 73 | S → A: Loss of proper localization to spindle pole and mitotic spindle. Significant increase in split spindle poles. 1 Publication | 1 | |
Mutagenesisi | 146 | T → A: Loss of proper localization to spindle pole and mitotic spindle. Significant increase in split spindle poles. 1 Publication | 1 | |
Mutagenesisi | 451 | P → A: Disrupts interaction with CBX5, loss of localization to centromeres in interphase, no effect on localization to centromeres in mitosis; when associated with A-453 and A-455. 1 Publication | 1 | |
Mutagenesisi | 453 | V → A: Disrupts interaction with CBX5, loss of localization to centromeres in interphase, no effect on localization to centromeres in mitosis; when associated with A-451 and A-455. 1 Publication | 1 | |
Mutagenesisi | 455 | I → A: Disrupts interaction with CBX5, loss of localization to centromeres in interphase, no effect on localization to centromeres in mitosis; when associated with A-451 and A-453. 1 Publication | 1 | |
Mutagenesisi | 492 | K → A: Loss of centromeric localization. 1 Publication | 1 | |
Mutagenesisi | 507 | S → A: Loss of phosphorylation; and presence of misaligned chromosomes; when associated with A-14. 1 Publication | 1 |
Keywords - Diseasei
Disease variantOrganism-specific databases
DisGeNETi | 151648 |
MalaCardsi | SGO1 |
MIMi | 616201, phenotype |
OpenTargetsi | ENSG00000129810 |
Orphaneti | 435988, Chronic atrial and intestinal dysrhythmia syndrome |
PharmGKBi | PA134988556 |
Miscellaneous databases
Pharosi | Q5FBB7, Tbio |
Genetic variation databases
BioMutai | SGO1 |
DMDMi | 74741474 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055436 | 1 – 561 | Shugoshin 1Add BLAST | 561 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | Phosphoserine; by NEK21 Publication | 1 | |
Modified residuei | 256 | PhosphoserineCombined sources | 1 | |
Modified residuei | 436 | PhosphoserineCombined sources | 1 | |
Modified residuei | 507 | Phosphoserine; by NEK2Combined sources1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q5FBB7 |
jPOSTi | Q5FBB7 |
MassIVEi | Q5FBB7 |
MaxQBi | Q5FBB7 |
PaxDbi | Q5FBB7 |
PeptideAtlasi | Q5FBB7 |
PRIDEi | Q5FBB7 |
ProteomicsDBi | 62781 [Q5FBB7-1] 62782 [Q5FBB7-2] 62783 [Q5FBB7-3] 62784 [Q5FBB7-4] 62785 [Q5FBB7-5] 62786 [Q5FBB7-6] 62787 [Q5FBB7-7] |
PTM databases
iPTMneti | Q5FBB7 |
PhosphoSitePlusi | Q5FBB7 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSG00000129810, Expressed in ventricular zone and 111 other tissues |
ExpressionAtlasi | Q5FBB7, baseline and differential |
Genevisiblei | Q5FBB7, HS |
Organism-specific databases
HPAi | ENSG00000129810, Tissue enhanced (bone marrow, lymphoid tissue, testis) |
Interactioni
Subunit structurei
Interacts with PPP2CA (or PPP2CB), PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E, SET, LRRC59, RBM10 (or RBM5), RPL10A, RPL28, RPL7, RPL7A and RPLP1. Interaction with protein phosphatase 2A occurs most probably through direct binding to the regulatory B56 subunits: PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E.
Interacts with PPP2R1A and NEK2.
Isoform 3 interacts with PLK1.
Interacts with CDCA8.
5 PublicationsBinary interactionsi
Q5FBB7
GO - Molecular functioni
- kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 127395, 105 interactors |
DIPi | DIP-36614N |
IntActi | Q5FBB7, 51 interactors |
MINTi | Q5FBB7 |
STRINGi | 9606.ENSP00000263753 |
Miscellaneous databases
RNActi | Q5FBB7, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q5FBB7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q5FBB7 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 176 | Necessary for interaction with PPP2CA and PPP2R1A1 PublicationAdd BLAST | 176 | |
Regioni | 260 – 331 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 348 – 441 | DisorderedSequence analysisAdd BLAST | 94 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 7 – 89 | Sequence analysisAdd BLAST | 83 | |
Coiled coili | 273 – 313 | Sequence analysisAdd BLAST | 41 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 192 – 200 | D-box 1 | 9 | |
Motifi | 310 – 312 | KEN box | 3 | |
Motifi | 438 – 446 | D-box 2 | 9 | |
Motifi | 451 – 455 | PXVXL/I motif1 Publication | 5 | |
Motifi | 457 – 465 | D-box 3 | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 269 – 294 | Basic and acidic residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 308 – 322 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 403 – 421 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 422 – 441 | Polar residuesSequence analysisAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000154107 |
HOGENOMi | CLU_1015500_0_0_1 |
InParanoidi | Q5FBB7 |
OMAi | CQWNKDQ |
OrthoDBi | 1414582at2759 |
PhylomeDBi | Q5FBB7 |
TreeFami | TF334213 |
Family and domain databases
InterProi | View protein in InterPro IPR028321, Sgo1 IPR038889, Shugoshin IPR011515, Shugoshin_C IPR011516, Shugoshin_N |
PANTHERi | PTHR21577, PTHR21577, 1 hit PTHR21577:SF3, PTHR21577:SF3, 1 hit |
Pfami | View protein in Pfam PF07557, Shugoshin_C, 1 hit PF07558, Shugoshin_N, 1 hit |
s (7+)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 7 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKERCLKKS FQDSLEDIKK RMKEKRNKNL AEIGKRRSFI AAPCQIITNT
60 70 80 90 100
STLLKNYQDN NKMLVLALEN EKSKVKEAQD IILQLRKECY YLTCQLYALK
110 120 130 140 150
GKLTSQQTVE PAQNQEICSS GMDPNSDDSS RNLFVKDLPQ IPLEETELPG
160 170 180 190 200
QGESFQIEDQ IPTIPQDTLG VDFDSGEAKS TDNVLPRTVS VRSSLKKHCN
210 220 230 240 250
SICQFDSLDD FETSHLAGKS FEFERVGFLD PLVNMHIPEN VQHNACQWSK
260 270 280 290 300
DQVNLSPKLI QPGTFTKTKE DILESKSEQT KSKQRDTQER KREEKRKANR
310 320 330 340 350
RKSKRMSKYK ENKSENKKTV PQKKMHKSVS SNDAYNFNLE EGVHLTPFRQ
360 370 380 390 400
KVSNDSNREE NNESEVSLCE SSGSGDDSDD LYLPTCKYIQ NPTSNSDRPV
410 420 430 440 450
TRPLAKRALK YTDEKETEGS KPTKTPTTTP PETQQSPHLS LKDITNVSLY
460 470 480 490 500
PVVKIRRLSL SPKKNKASPA VALPKRRCTA SVNYKEPTLA SKLRRGDPFT
510 520 530 540 550
DLCFLNSPIF KQKKDLRRSK KRALEVSPAK EAIFILYYVR EFVSRFPDCR
560
KCKLETHICL R
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF8WB17 | F8WB17_HUMAN | Shugoshin 1 | SGO1 | 84 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_072709 | 23 | K → E in CAID; patient fibroblasts exhibit significantly faster cell proliferation than controls; during mitosis the mutant protein is localized in an ordered fashion around the centromeres but display a rather homogeneous cytoplasmic localization pattern. 1 PublicationCorresponds to variant dbSNP:rs199815268EnsemblClinVar. | 1 | |
Natural variantiVAR_051968 | 171 | V → A. Corresponds to variant dbSNP:rs6806241EnsemblClinVar. | 1 | |
Natural variantiVAR_051969 | 322 | Q → P. Corresponds to variant dbSNP:rs9868701EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016790 | 159 | D → A in isoform 3 and isoform 5. 3 Publications | 1 | |
Alternative sequenceiVSP_016791 | 160 – 428 | Missing in isoform 3 and isoform 5. 3 PublicationsAdd BLAST | 269 | |
Alternative sequenceiVSP_016792 | 176 | G → A in isoform 2 and isoform 4. 2 Publications | 1 | |
Alternative sequenceiVSP_016793 | 177 – 522 | Missing in isoform 7. 1 PublicationAdd BLAST | 346 | |
Alternative sequenceiVSP_016794 | 177 – 428 | Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST | 252 | |
Alternative sequenceiVSP_016795 | 522 – 561 | RALEV…HICLR → SMKQIQ in isoform 4, isoform 5 and isoform 6. 4 PublicationsAdd BLAST | 40 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FGA | X-ray | 2.70 | D | 51-96 | [»] | |
3Q6S | X-ray | 1.93 | E/F | 445-463 | [»] | |
4A0I | X-ray | 2.60 | C/D | 2-6 | [»] | |
SMRi | Q5FBB7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 127395, 105 interactors |
DIPi | DIP-36614N |
IntActi | Q5FBB7, 51 interactors |
MINTi | Q5FBB7 |
STRINGi | 9606.ENSP00000263753 |
PTM databases
iPTMneti | Q5FBB7 |
PhosphoSitePlusi | Q5FBB7 |
Genetic variation databases
BioMutai | SGO1 |
DMDMi | 74741474 |
Proteomic databases
EPDi | Q5FBB7 |
jPOSTi | Q5FBB7 |
MassIVEi | Q5FBB7 |
MaxQBi | Q5FBB7 |
PaxDbi | Q5FBB7 |
PeptideAtlasi | Q5FBB7 |
PRIDEi | Q5FBB7 |
ProteomicsDBi | 62781 [Q5FBB7-1] 62782 [Q5FBB7-2] 62783 [Q5FBB7-3] 62784 [Q5FBB7-4] 62785 [Q5FBB7-5] 62786 [Q5FBB7-6] 62787 [Q5FBB7-7] |
Protocols and materials databases
Antibodypediai | 27089, 239 antibodies from 27 providers |
DNASUi | 151648 |
Genome annotation databases
Organism-specific databases
CTDi | 151648 |
DisGeNETi | 151648 |
GeneCardsi | SGO1 |
HGNCi | HGNC:25088, SGO1 |
HPAi | ENSG00000129810, Tissue enhanced (bone marrow, lymphoid tissue, testis) |
MalaCardsi | SGO1 |
MIMi | 609168, gene 616201, phenotype |
neXtProti | NX_Q5FBB7 |
OpenTargetsi | ENSG00000129810 |
Orphaneti | 435988, Chronic atrial and intestinal dysrhythmia syndrome |
PharmGKBi | PA134988556 |
VEuPathDBi | HostDB:ENSG00000129810 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000154107 |
HOGENOMi | CLU_1015500_0_0_1 |
InParanoidi | Q5FBB7 |
OMAi | CQWNKDQ |
OrthoDBi | 1414582at2759 |
PhylomeDBi | Q5FBB7 |
TreeFami | TF334213 |
Enzyme and pathway databases
PathwayCommonsi | Q5FBB7 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q5FBB7 |
SIGNORi | Q5FBB7 |
Miscellaneous databases
BioGRID-ORCSi | 151648, 694 hits in 1005 CRISPR screens |
ChiTaRSi | SGO1, human |
EvolutionaryTracei | Q5FBB7 |
GeneWikii | SGOL1 |
GenomeRNAii | 151648 |
Pharosi | Q5FBB7, Tbio |
PROi | PR:Q5FBB7 |
RNActi | Q5FBB7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000129810, Expressed in ventricular zone and 111 other tissues |
ExpressionAtlasi | Q5FBB7, baseline and differential |
Genevisiblei | Q5FBB7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR028321, Sgo1 IPR038889, Shugoshin IPR011515, Shugoshin_C IPR011516, Shugoshin_N |
PANTHERi | PTHR21577, PTHR21577, 1 hit PTHR21577:SF3, PTHR21577:SF3, 1 hit |
Pfami | View protein in Pfam PF07557, Shugoshin_C, 1 hit PF07558, Shugoshin_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SGO1_HUMAN | |
Accessioni | Q5FBB7Primary (citable) accession number: Q5FBB7 Secondary accession number(s): Q588H5 Q9H275 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2006 |
Last sequence update: | March 15, 2005 | |
Last modified: | February 23, 2022 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families