UniProtKB - Q5F3T9 (UGDH_CHICK)
Protein
UDP-glucose 6-dehydrogenase
Gene
UGDH
Organism
Gallus gallus (Chicken)
Status
Functioni
Catalyzes the formation of UDP-alpha-D-glucuronate, a constituent of complex glycosaminoglycans (By similarity). Required for the biosynthesis of chondroitin sulfate and heparan sulfate. Required for embryonic development via its role in the biosynthesis of glycosaminoglycans (By similarity).By similarity
Catalytic activityi
- EC:1.1.1.22By similarity
Activity regulationi
UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor. It binds at the same site as the substrate, but functions as allosteric inhibitor by triggering a conformation change that disrupts the active hexameric ring structure and gives rise to an inactive, horseshoe-shaped hexamer.By similarity
: UDP-alpha-D-glucuronate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose.By similarityProteins known to be involved in this subpathway in this organism are:
- UDP-glucose 6-dehydrogenase (UGDH)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose, the pathway UDP-alpha-D-glucuronate biosynthesis and in Nucleotide-sugar biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 36 | NADBy similarity | 1 | |
Binding sitei | 41 | NADBy similarity | 1 | |
Active sitei | 161 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 165 | NADBy similarity | 1 | |
Active sitei | 220 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 260 | SubstrateBy similarity | 1 | |
Active sitei | 276 | NucleophileBy similarity | 1 | |
Binding sitei | 346 | NADBy similarity | 1 | |
Binding sitei | 442 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 16 | NADBy similarity | 6 | |
Nucleotide bindingi | 89 – 93 | NADBy similarity | 5 | |
Nucleotide bindingi | 130 – 132 | NADBy similarity | 3 | |
Nucleotide bindingi | 276 – 279 | NADBy similarity | 4 |
GO - Molecular functioni
- identical protein binding Source: Ensembl
- NAD binding Source: InterPro
- UDP-glucose 6-dehydrogenase activity Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
- chondroitin sulfate biosynthetic process Source: UniProtKB
- gastrulation with mouth forming second Source: AgBase
- glycosaminoglycan biosynthetic process Source: GO_Central
- heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
- neuron development Source: Ensembl
- protein hexamerization Source: UniProtKB
- UDP-glucuronate biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Allosteric enzyme, Oxidoreductase |
Biological process | Carbohydrate metabolism |
Ligand | NAD |
Enzyme and pathway databases
Reactomei | R-GGA-173599, Formation of the active cofactor, UDP-glucuronate |
UniPathwayi | UPA00038;UER00491 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-glucose 6-dehydrogenase (EC:1.1.1.22By similarity)Short name: UDP-Glc dehydrogenase Short name: UDP-GlcDH Short name: UDPGDH |
Gene namesi | Name:UGDH ORF Names:RCJMB04_7d7 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000317478 | 1 – 494 | UDP-glucose 6-dehydrogenaseAdd BLAST | 494 |
Proteomic databases
PaxDbi | Q5F3T9 |
Expressioni
Gene expression databases
Bgeei | ENSGALG00000014320, Expressed in liver and 13 other tissues |
Interactioni
Subunit structurei
Homohexamer.
By similarityGO - Molecular functioni
- identical protein binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000023101 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 88 – 110 | Intrinsically disordered; important for formation of active hexamer structureBy similarityAdd BLAST | 23 | |
Regioni | 129 – 135 | Allosteric switch regionBy similarity | 7 | |
Regioni | 161 – 165 | Substrate bindingBy similarity | 5 | |
Regioni | 220 – 224 | Substrate bindingBy similarity | 5 | |
Regioni | 267 – 273 | Substrate bindingBy similarity | 7 | |
Regioni | 321 – 325 | Important for formation of active hexamer structureBy similarity | 5 | |
Regioni | 338 – 339 | Substrate bindingBy similarity | 2 | |
Regioni | 466 – 494 | Intrinsically disorderedBy similarityAdd BLAST | 29 |
Domaini
The protein goes through several conformation states during the reaction cycle, giving rise to hysteresis. In the initial state, the ligand-free protein is in an inactive conformation (E*). Substrate binding triggers a change to the active conformation (E). UDP-xylose binding triggers the transition to a distinct, inhibited conformation. The presence of an intrinsically disordered C-terminus promotes a more dynamic protein structure and favors a conformation with high affinity for UPD-xylose.By similarity
The allosteric switch region moves by about 5 Angstroms when UDP-xylose is bound, and occupies part of the UDP-glucose binding site. At the same time it promotes domain movements that disrupt the active hexameric ring structure and lead to the formation of a horseshoe-shaped, inactive hexamer.By similarity
Sequence similaritiesi
Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG2666, Eukaryota |
GeneTreei | ENSGT00390000015355 |
HOGENOMi | CLU_023810_7_2_1 |
InParanoidi | Q5F3T9 |
OMAi | LCTEWDE |
OrthoDBi | 915490at2759 |
PhylomeDBi | Q5F3T9 |
Family and domain databases
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf IPR017476, UDP-Glc/GDP-Man IPR014027, UDP-Glc/GDP-Man_DH_C IPR036220, UDP-Glc/GDP-Man_DH_C_sf IPR014026, UDP-Glc/GDP-Man_DH_dimer IPR001732, UDP-Glc/GDP-Man_DH_N IPR028356, UDPglc_DH_euk |
PANTHERi | PTHR11374, PTHR11374, 1 hit |
Pfami | View protein in Pfam PF00984, UDPG_MGDP_dh, 1 hit PF03720, UDPG_MGDP_dh_C, 1 hit PF03721, UDPG_MGDP_dh_N, 1 hit |
PIRSFi | PIRSF500133, UDPglc_DH_euk, 1 hit PIRSF000124, UDPglc_GDPman_dh, 1 hit |
SMARTi | View protein in SMART SM00984, UDPG_MGDP_dh_C, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit SSF52413, SSF52413, 1 hit |
TIGRFAMsi | TIGR03026, NDP-sugDHase, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5F3T9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFEIKKICCI GAGYVGGPTC SVIAQMCPKI QVTVVDVNEA RINAWNSDTL
60 70 80 90 100
PIYEPGLKEV VESCRGRNLF FSTSIDDAIR EADLVFISVN TPTKTYGMGK
110 120 130 140 150
GRAADLKYIE ACARRIVQNS NGYKIVTEKS TVPVRAAESI RRIFDANTKP
160 170 180 190 200
NLDLQVLSNP EFLAEGTAIK DLKNPDRVLI GGDDSPEGQK AVRALCAVYE
210 220 230 240 250
HWVPKEKILT TNTWSSELSK LAANAFLAQR ISSINSISAL CEATGADVEE
260 270 280 290 300
VARAIGTDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW
310 320 330 340 350
QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS
360 370 380 390 400
IYISKYLMDE GAKLHIYDPK VPKEQIILDL SHPGVSEDNQ VSRLVTISQD
410 420 430 440 450
PYEACDGAHA LVICTEWDMF KELDYERIHK KMLKPAFIFD GRRVLDDLHN
460 470 480 490
ELQVIGFQIE TIGKKVSAKR IPFASSCEIP KFSLQDPPVK KPRV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ851561 mRNA Translation: CAH65195.1 |
RefSeqi | NP_001012599.1, NM_001012581.1 |
Genome annotation databases
Ensembli | ENSGALT00000090339; ENSGALP00000060119; ENSGALG00000041993 |
GeneIDi | 422792 |
KEGGi | gga:422792 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ851561 mRNA Translation: CAH65195.1 |
RefSeqi | NP_001012599.1, NM_001012581.1 |
3D structure databases
SMRi | Q5F3T9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000023101 |
Proteomic databases
PaxDbi | Q5F3T9 |
Genome annotation databases
Ensembli | ENSGALT00000090339; ENSGALP00000060119; ENSGALG00000041993 |
GeneIDi | 422792 |
KEGGi | gga:422792 |
Organism-specific databases
CTDi | 7358 |
Phylogenomic databases
eggNOGi | KOG2666, Eukaryota |
GeneTreei | ENSGT00390000015355 |
HOGENOMi | CLU_023810_7_2_1 |
InParanoidi | Q5F3T9 |
OMAi | LCTEWDE |
OrthoDBi | 915490at2759 |
PhylomeDBi | Q5F3T9 |
Enzyme and pathway databases
UniPathwayi | UPA00038;UER00491 |
Reactomei | R-GGA-173599, Formation of the active cofactor, UDP-glucuronate |
Miscellaneous databases
PROi | PR:Q5F3T9 |
Gene expression databases
Bgeei | ENSGALG00000014320, Expressed in liver and 13 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf IPR017476, UDP-Glc/GDP-Man IPR014027, UDP-Glc/GDP-Man_DH_C IPR036220, UDP-Glc/GDP-Man_DH_C_sf IPR014026, UDP-Glc/GDP-Man_DH_dimer IPR001732, UDP-Glc/GDP-Man_DH_N IPR028356, UDPglc_DH_euk |
PANTHERi | PTHR11374, PTHR11374, 1 hit |
Pfami | View protein in Pfam PF00984, UDPG_MGDP_dh, 1 hit PF03720, UDPG_MGDP_dh_C, 1 hit PF03721, UDPG_MGDP_dh_N, 1 hit |
PIRSFi | PIRSF500133, UDPglc_DH_euk, 1 hit PIRSF000124, UDPglc_GDPman_dh, 1 hit |
SMARTi | View protein in SMART SM00984, UDPG_MGDP_dh_C, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit SSF52413, SSF52413, 1 hit |
TIGRFAMsi | TIGR03026, NDP-sugDHase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UGDH_CHICK | |
Accessioni | Q5F3T9Primary (citable) accession number: Q5F3T9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | March 15, 2005 | |
Last modified: | December 2, 2020 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families