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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7

Gene

Enpp7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts sphingomyelin to ceramide and phosphocholine (PubMed:15708357). Can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine. Does not have nucleotide pyrophosphatase activity (By similarity).By similarity1 Publication

Catalytic activityi

Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.1 Publication

Cofactori

Zn2+By similarity

pH dependencei

Optimum pH is 9.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi38Zinc 1By similarity1
Active sitei74NucleophileBy similarity1
Metal bindingi74Zinc 1By similarity1
Binding sitei95SubstrateBy similarity1
Metal bindingi198Zinc 2By similarity1
Metal bindingi202Zinc 2; via tele nitrogenBy similarity1
Metal bindingi245Zinc 1By similarity1
Metal bindingi246Zinc 1; via tele nitrogenBy similarity1
Metal bindingi352Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (EC:3.1.4.121 Publication)
Short name:
E-NPP 7
Short name:
NPP-7
Alternative name(s):
Alkaline sphingomyelin phosphodiesterase
Intestinal alkaline sphingomyelinase
Short name:
Alk-SMase
Gene namesi
Name:Enpp7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1359324 Enpp7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 422Extracellular1 PublicationAdd BLAST401
Transmembranei423 – 439HelicalSequence analysisAdd BLAST17

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000003640422 – 439Ectonucleotide pyrophosphatase/phosphodiesterase family member 7Add BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi266N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated; required for activity and transport to the plasma membrane.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5EZ72
PRIDEiQ5EZ72

Expressioni

Tissue specificityi

Detected in small intestine (at protein level) (PubMed:15205117). Highly expressed in the jejunum (PubMed:15708357).2 Publications

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064040

Structurei

3D structure databases

ProteinModelPortaliQ5EZ72
SMRiQ5EZ72
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 77Required for enzyme activityBy similarity7

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2645 Eukaryota
COG1524 LUCA
HOVERGENiHBG106748
InParanoidiQ5EZ72
KOiK12354
PhylomeDBiQ5EZ72

Family and domain databases

Gene3Di3.40.720.10, 2 hits
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR029895 ENPP7
IPR002591 Phosphodiest/P_Trfase
PANTHERiPTHR10151:SF63 PTHR10151:SF63, 1 hit
PfamiView protein in Pfam
PF01663 Phosphodiest, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q5EZ72-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGHSAVLLSV ALVILPACVT GGPVQRQQQH KLLLVSFDGF RWNYDQDVET
60 70 80 90 100
PNLDSMAQEG VKARYMTPAF VTMTSPCHFT LVTGKYIENH GVVHNMFYNT
110 120 130 140 150
TNKVRLPYHA TLGIQRWWDN GSIPIWITAQ RQGLKTGSFF YPGGNVTYQG
160 170 180 190 200
EAVTMSRKEG VLHNYKNETE WRANVDTVMK WFTEEDVSLV TLYFGEPDST
210 220 230 240 250
GHKYGPESQE RKDMVKQVDR TVGYLRDSIK RHHLTDSLNL IITSDHGMTT
260 270 280 290 300
VNKKASDLVE FHKFPNFTFR DIEFELLDYG PNGMLIPKEG MLEKVYSVLK
310 320 330 340 350
DAHPRLHVYK KEDFPKTFHY ANNPRITSLL MYSDLGYVIH GRVNVQFNSG
360 370 380 390 400
EHGFDNQDMD MKTIFRAVGP SFKAGLEVEP FESVHVYELM CQLLGIVPEP
410 420 430
NDGHPGVLQP MLRSGSPLSR QHHLVVVLMG ILTGLAKVV
Length:439
Mass (Da):49,837
Last modified:March 15, 2005 - v1
Checksum:i7CEABF0393C9480F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0R3W7M0R3W7_RAT
Ectonucleotide pyrophosphatase/phos...
Enpp7
439Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY568760 mRNA Translation: AAU03450.1
RefSeqiNP_001012484.1, NM_001012466.1
UniGeneiRn.211790

Genome annotation databases

GeneIDi303729
KEGGirno:303729

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY568760 mRNA Translation: AAU03450.1
RefSeqiNP_001012484.1, NM_001012466.1
UniGeneiRn.211790

3D structure databases

ProteinModelPortaliQ5EZ72
SMRiQ5EZ72
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064040

Proteomic databases

PaxDbiQ5EZ72
PRIDEiQ5EZ72

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi303729
KEGGirno:303729

Organism-specific databases

CTDi339221
RGDi1359324 Enpp7

Phylogenomic databases

eggNOGiKOG2645 Eukaryota
COG1524 LUCA
HOVERGENiHBG106748
InParanoidiQ5EZ72
KOiK12354
PhylomeDBiQ5EZ72

Miscellaneous databases

PROiPR:Q5EZ72

Family and domain databases

Gene3Di3.40.720.10, 2 hits
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR017850 Alkaline_phosphatase_core_sf
IPR029895 ENPP7
IPR002591 Phosphodiest/P_Trfase
PANTHERiPTHR10151:SF63 PTHR10151:SF63, 1 hit
PfamiView protein in Pfam
PF01663 Phosphodiest, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiENPP7_RAT
AccessioniPrimary (citable) accession number: Q5EZ72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 15, 2005
Last modified: June 20, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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