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Protein

Caspase recruitment domain-containing protein 16

Gene

CARD16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.3 Publications

GO - Molecular functioni

  • CARD domain binding Source: UniProtKB
  • caspase binding Source: UniProtKB
  • cysteine-type endopeptidase inhibitor activity Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • kinase binding Source: UniProtKB

GO - Biological processi

  • cellular response to hypoxia Source: UniProtKB
  • cellular response to lipopolysaccharide Source: UniProtKB
  • cellular response to UV-C Source: UniProtKB
  • inhibition of cysteine-type endopeptidase activity Source: UniProtKB
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • negative regulation of interleukin-1 beta secretion Source: UniProtKB
  • negative regulation of lipopolysaccharide-mediated signaling pathway Source: UniProtKB
  • negative regulation of protein binding Source: UniProtKB
  • negative regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB

Keywordsi

Molecular functionProtease inhibitor, Thiol protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase recruitment domain-containing protein 16
Alternative name(s):
Caspase recruitment domain-only protein 1
Short name:
CARD-only protein 1
Caspase-1 inhibitor COP
Pseudo interleukin-1 beta converting enzyme
Short name:
Pseudo-ICE
Short name:
Pseudo-IL1B-converting enzyme
Gene namesi
Name:CARD16
Synonyms:COP, COP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000204397.7
HGNCiHGNC:33701 CARD16
MIMi615680 gene
neXtProtiNX_Q5EG05

Subcellular locationi

Pathology & Biotechi

Organism-specific databases

DisGeNETi114769
OpenTargetsiENSG00000204397
PharmGKBiPA164717628

Polymorphism and mutation databases

BioMutaiCARD16
DMDMi74722547

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003491801 – 197Caspase recruitment domain-containing protein 16Add BLAST197

Proteomic databases

MaxQBiQ5EG05
PaxDbiQ5EG05
PeptideAtlasiQ5EG05
PRIDEiQ5EG05
ProteomicsDBi62773
62774 [Q5EG05-2]

PTM databases

iPTMnetiQ5EG05
PhosphoSitePlusiQ5EG05

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in placenta, spleen, lymph node and bone marrow. Weakly or not expressed in thymus.2 Publications

Inductioni

Down-regulated in patients suffering of Huntington disease.1 Publication

Gene expression databases

BgeeiENSG00000204397
CleanExiHS_CARD16
ExpressionAtlasiQ5EG05 baseline and differential
GenevisibleiQ5EG05 HS

Organism-specific databases

HPAiHPA053981
HPA062805

Interactioni

Subunit structurei

Homooligomer. Interacts with CASP1, CASP4, CARD8 and RIPK2.4 Publications

GO - Molecular functioni

  • CARD domain binding Source: UniProtKB
  • caspase binding Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi125339, 3 interactors
IntActiQ5EG05, 1 interactor
MINTiQ5EG05
STRINGi9606.ENSP00000364858

Structurei

3D structure databases

ProteinModelPortaliQ5EG05
SMRiQ5EG05
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 91CARDPROSITE-ProRule annotationAdd BLAST91

Phylogenomic databases

eggNOGiKOG3573 Eukaryota
ENOG410ZQIE LUCA
GeneTreeiENSGT00510000049857
HOGENOMiHOG000111300
HOVERGENiHBG006113
InParanoidiQ5EG05
KOiK12806
OMAiRTNFIER
OrthoDBiEOG091G0L5Y
PhylomeDBiQ5EG05
TreeFamiTF330675

Family and domain databases

InterProiView protein in InterPro
IPR001315 CARD
IPR011029 DEATH-like_dom_sf
PfamiView protein in Pfam
PF00619 CARD, 1 hit
SMARTiView protein in SMART
SM00114 CARD, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
PROSITEiView protein in PROSITE
PS50209 CARD, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5EG05-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADKVLKEKR KLFIHSMGEG TINGLLDELL QTRVLNQEEM EKVKRENATV
60 70 80 90 100
MDKTRALIDS VIPKGAQACQ ICITYICEED SYLAETLGLS AALQAVQDNP
110 120 130 140 150
AMPTCSSPEG RIKLCFLEDA QRIWKQKLQR CHVQNTIIKW SERYTSGSFE
160 170 180 190
MQWLFLRTNF IERFWRNILL LPLHKGSLYP RIPGLGKELQ TGTHKLS
Length:197
Mass (Da):22,625
Last modified:March 15, 2005 - v1
Checksum:i5DCAC6A9B2FAE82F
GO
Isoform 2 (identifier: Q5EG05-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     92-197: ALQAVQDNPA...ELQTGTHKLS → GPIPGN

Show »
Length:97
Mass (Da):10,737
Checksum:i8697C3B5706B9B91
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04627933R → S. Corresponds to variant dbSNP:rs35966314Ensembl.1
Natural variantiVAR_04628037Q → K. Corresponds to variant dbSNP:rs1042744Ensembl.1
Natural variantiVAR_04628156A → D. Corresponds to variant dbSNP:rs34534919Ensembl.1
Natural variantiVAR_046282167N → I. Corresponds to variant dbSNP:rs542571Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03521692 – 197ALQAV…THKLS → GPIPGN in isoform 2. 3 PublicationsAdd BLAST106

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF367017 mRNA Translation: AAK71682.1
AY885669 mRNA Translation: AAW78563.1
AK311902 mRNA Translation: BAG34843.1
CH471065 Genomic DNA Translation: EAW67062.1
BC117478 mRNA Translation: AAI17479.1
BC117480 mRNA Translation: AAI17481.1
CCDSiCCDS31661.1 [Q5EG05-1]
CCDS41705.1 [Q5EG05-2]
RefSeqiNP_001017534.1, NM_001017534.1 [Q5EG05-1]
NP_443121.1, NM_052889.2 [Q5EG05-2]
UniGeneiHs.348365

Genome annotation databases

EnsembliENST00000375704; ENSP00000364856; ENSG00000204397 [Q5EG05-2]
ENST00000375706; ENSP00000364858; ENSG00000204397 [Q5EG05-1]
ENST00000525374; ENSP00000433700; ENSG00000204397 [Q5EG05-2]
GeneIDi114769
KEGGihsa:114769
UCSCiuc001pio.2 human [Q5EG05-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCAR16_HUMAN
AccessioniPrimary (citable) accession number: Q5EG05
Secondary accession number(s): Q96RJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 15, 2005
Last modified: June 20, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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