UniProtKB - Q5EBM0 (CMPK2_HUMAN)
UMP-CMP kinase 2, mitochondrial
CMPK2
Functioni
May participate in dUTP and dCTP synthesis in mitochondria. Is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. May be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs. Also displays broad nucleoside diphosphate kinase activity.
2 PublicationsCatalytic activityi
- EC:2.7.4.141 Publication
- EC:2.7.4.141 Publication
- a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP1 PublicationEC:2.7.4.61 Publication
- EC:2.7.4.61 Publication
Kineticsi
- KM=3.09 mM for CMP
- KM=6.3 mM for UMP
- KM=1.31 mM for dCMP
- KM=0.1 mM for dUMP
- Vmax=1.64 µmol/min/mg enzyme towards CMP
- Vmax=0.19 µmol/min/mg enzyme towards UMP
- Vmax=1.77 µmol/min/mg enzyme towards dCMP
- Vmax=0.48 µmol/min/mg enzyme towards dUMP
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 259 – 266 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- CMP kinase activity Source: RHEA
- cytidylate kinase activity Source: MGI
- dCMP kinase activity Source: RHEA
- nucleoside diphosphate kinase activity Source: UniProtKB
- thymidylate kinase activity Source: GO_Central
- UMP kinase activity Source: MGI
- uridylate kinase activity Source: GO_Central
GO - Biological processi
- cellular response to lipopolysaccharide Source: Ensembl
- dTDP biosynthetic process Source: GO_Central
- dTTP biosynthetic process Source: GO_Central
- dUDP biosynthetic process Source: GO_Central
- nucleoside diphosphate phosphorylation Source: UniProtKB
- nucleoside triphosphate biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Pyrimidine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.4.14, 2681 |
PathwayCommonsi | Q5EBM0 |
SABIO-RKi | Q5EBM0 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CMPK2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:27015, CMPK2 |
MIMi | 611787, gene |
neXtProti | NX_Q5EBM0 |
VEuPathDBi | HostDB:ENSG00000134326 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrion Source: HPA
Nucleus
- nucleoplasm Source: HPA
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Organism-specific databases
DisGeNETi | 129607 |
OpenTargetsi | ENSG00000134326 |
PharmGKBi | PA162382556 |
Miscellaneous databases
Pharosi | Q5EBM0, Tbio |
Genetic variation databases
BioMutai | CMPK2 |
DMDMi | 296439392 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 98 | MitochondrionSequence analysisAdd BLAST | 98 | |
ChainiPRO_0000331524 | 99 – 449 | UMP-CMP kinase 2, mitochondrialAdd BLAST | 351 |
Proteomic databases
EPDi | Q5EBM0 |
jPOSTi | Q5EBM0 |
MassIVEi | Q5EBM0 |
MaxQBi | Q5EBM0 |
PaxDbi | Q5EBM0 |
PeptideAtlasi | Q5EBM0 |
PRIDEi | Q5EBM0 |
ProteomicsDBi | 62766 [Q5EBM0-1] 62767 [Q5EBM0-2] 62768 [Q5EBM0-3] 62769 [Q5EBM0-4] |
PTM databases
iPTMneti | Q5EBM0 |
PhosphoSitePlusi | Q5EBM0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000134326, Expressed in epithelial cell of pancreas and 211 other tissues |
Genevisiblei | Q5EBM0, HS |
Organism-specific databases
HPAi | ENSG00000134326, Tissue enhanced (brain, salivary gland) |
Interactioni
Protein-protein interaction databases
STRINGi | 9606.ENSP00000256722 |
Miscellaneous databases
RNActi | Q5EBM0, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 380 – 412 | Sequence analysisAdd BLAST | 33 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transit peptidePhylogenomic databases
eggNOGi | KOG3327, Eukaryota |
GeneTreei | ENSGT00940000154030 |
HOGENOMi | CLU_049896_0_0_1 |
InParanoidi | Q5EBM0 |
OMAi | MAPPRCF |
OrthoDBi | 1450196at2759 |
PhylomeDBi | Q5EBM0 |
TreeFami | TF328875 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR039430, Thymidylate_kin-like_dom IPR014505, UMP-CMP_kinase_2 |
Pfami | View protein in Pfam PF02223, Thymidylate_kin, 1 hit |
PIRSFi | PIRSF019736, dTMP_TKRP1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAFARRLLRG PLSGPLLGRR GVCAGAMAPP RRFVLELPDC TLAHFALGAD
60 70 80 90 100
APGDADAPDP RLAALLGPPE RSYSLCVPVT PDAGCGARVR AARLHQRLLH
110 120 130 140 150
QLRRGPFQRC QLLRLLCYCP GGQAGGAQQG FLLRDPLDDP DTRQALLELL
160 170 180 190 200
GACQEAPRPH LGEFEADPRG QLWQRLWEVQ DGRRLQVGCA QVVPVPEPPL
210 220 230 240 250
HPVVPDLPSS VVFPDREAAR AVLEECTSFI PEARAVLDLV DQCPKQIQKG
260 270 280 290 300
KFQVVAIEGL DATGKTTVTQ SVADSLKAVL LKSPPSCIGQ WRKIFDDEPT
310 320 330 340 350
IIRRAFYSLG NYIVASEIAK ESAKSPVIVD RYWHSTATYA IATEVSGGLQ
360 370 380 390 400
HLPPAHHPVY QWPEDLLKPD LILLLTVSPE ERLQRLQGRG MEKTREEAEL
410 420 430 440
EANSVFRQKV EMSYQRMENP GCHVVDASPS REKVLQTVLS LIQNSFSEP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 448 | E → G in AAH89425 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055997 | 433 | K → R. Corresponds to variant dbSNP:rs6712141Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_033238 | 280 – 303 | LLKSP…PTIIR → PQPITLCTSGQRTCSNLTLS CCSL in isoform 2. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_033239 | 304 – 449 | Missing in isoform 2. 1 PublicationAdd BLAST | 146 | |
Alternative sequenceiVSP_033240 | 332 – 449 | YWHST…SFSEP → SQLGGTLYHPSLHLLGSEVC GTGILDSSHSSQGLE in isoform 3. 1 PublicationAdd BLAST | 118 | |
Alternative sequenceiVSP_033241 | 410 – 449 | Missing in isoform 4. 1 PublicationAdd BLAST | 40 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK127983 mRNA Translation: BAC87217.1 BC016969 mRNA Translation: AAH16969.1 BC141802 mRNA Translation: AAI41803.1 BC132821 mRNA Translation: AAI32822.1 BC089425 mRNA Translation: AAH89425.1 BC144202 mRNA Translation: AAI44203.1 |
CCDSi | CCDS42648.1 [Q5EBM0-1] CCDS58695.1 [Q5EBM0-3] CCDS58696.1 [Q5EBM0-4] |
RefSeqi | NP_001243406.1, NM_001256477.1 [Q5EBM0-4] NP_001243407.1, NM_001256478.1 [Q5EBM0-3] NP_997198.2, NM_207315.3 [Q5EBM0-1] |
Genome annotation databases
Ensembli | ENST00000256722.10; ENSP00000256722.5; ENSG00000134326.12 ENST00000404168.1; ENSP00000384915.1; ENSG00000134326.12 [Q5EBM0-4] ENST00000458098.5; ENSP00000396385.1; ENSG00000134326.12 [Q5EBM0-3] |
GeneIDi | 129607 |
KEGGi | hsa:129607 |
MANE-Selecti | ENST00000256722.10; ENSP00000256722.5; NM_207315.4; NP_997198.2 |
UCSCi | uc002qyo.5, human [Q5EBM0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK127983 mRNA Translation: BAC87217.1 BC016969 mRNA Translation: AAH16969.1 BC141802 mRNA Translation: AAI41803.1 BC132821 mRNA Translation: AAI32822.1 BC089425 mRNA Translation: AAH89425.1 BC144202 mRNA Translation: AAI44203.1 |
CCDSi | CCDS42648.1 [Q5EBM0-1] CCDS58695.1 [Q5EBM0-3] CCDS58696.1 [Q5EBM0-4] |
RefSeqi | NP_001243406.1, NM_001256477.1 [Q5EBM0-4] NP_001243407.1, NM_001256478.1 [Q5EBM0-3] NP_997198.2, NM_207315.3 [Q5EBM0-1] |
3D structure databases
AlphaFoldDBi | Q5EBM0 |
SMRi | Q5EBM0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9606.ENSP00000256722 |
PTM databases
iPTMneti | Q5EBM0 |
PhosphoSitePlusi | Q5EBM0 |
Genetic variation databases
BioMutai | CMPK2 |
DMDMi | 296439392 |
Proteomic databases
EPDi | Q5EBM0 |
jPOSTi | Q5EBM0 |
MassIVEi | Q5EBM0 |
MaxQBi | Q5EBM0 |
PaxDbi | Q5EBM0 |
PeptideAtlasi | Q5EBM0 |
PRIDEi | Q5EBM0 |
ProteomicsDBi | 62766 [Q5EBM0-1] 62767 [Q5EBM0-2] 62768 [Q5EBM0-3] 62769 [Q5EBM0-4] |
Protocols and materials databases
Antibodypediai | 26409, 86 antibodies from 20 providers |
DNASUi | 129607 |
Genome annotation databases
Ensembli | ENST00000256722.10; ENSP00000256722.5; ENSG00000134326.12 ENST00000404168.1; ENSP00000384915.1; ENSG00000134326.12 [Q5EBM0-4] ENST00000458098.5; ENSP00000396385.1; ENSG00000134326.12 [Q5EBM0-3] |
GeneIDi | 129607 |
KEGGi | hsa:129607 |
MANE-Selecti | ENST00000256722.10; ENSP00000256722.5; NM_207315.4; NP_997198.2 |
UCSCi | uc002qyo.5, human [Q5EBM0-1] |
Organism-specific databases
CTDi | 129607 |
DisGeNETi | 129607 |
GeneCardsi | CMPK2 |
HGNCi | HGNC:27015, CMPK2 |
HPAi | ENSG00000134326, Tissue enhanced (brain, salivary gland) |
MIMi | 611787, gene |
neXtProti | NX_Q5EBM0 |
OpenTargetsi | ENSG00000134326 |
PharmGKBi | PA162382556 |
VEuPathDBi | HostDB:ENSG00000134326 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3327, Eukaryota |
GeneTreei | ENSGT00940000154030 |
HOGENOMi | CLU_049896_0_0_1 |
InParanoidi | Q5EBM0 |
OMAi | MAPPRCF |
OrthoDBi | 1450196at2759 |
PhylomeDBi | Q5EBM0 |
TreeFami | TF328875 |
Enzyme and pathway databases
BRENDAi | 2.7.4.14, 2681 |
PathwayCommonsi | Q5EBM0 |
SABIO-RKi | Q5EBM0 |
Miscellaneous databases
BioGRID-ORCSi | 129607, 16 hits in 1084 CRISPR screens |
GenomeRNAii | 129607 |
Pharosi | Q5EBM0, Tbio |
PROi | PR:Q5EBM0 |
RNActi | Q5EBM0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000134326, Expressed in epithelial cell of pancreas and 211 other tissues |
Genevisiblei | Q5EBM0, HS |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR039430, Thymidylate_kin-like_dom IPR014505, UMP-CMP_kinase_2 |
Pfami | View protein in Pfam PF02223, Thymidylate_kin, 1 hit |
PIRSFi | PIRSF019736, dTMP_TKRP1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CMPK2_HUMAN | |
Accessioni | Q5EBM0Primary (citable) accession number: Q5EBM0 Secondary accession number(s): A2RUB0 Q96AL8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | May 18, 2010 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families