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Protein

Ras association domain-containing protein 5

Gene

Rassf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Potental tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing (By similarity). May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri117 – 165Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ras association domain-containing protein 5
Alternative name(s):
New ras effector 1
Regulator for cell adhesion and polarization enriched in lymphoid tissues
Short name:
RAPL
Gene namesi
Name:Rassf5
Synonyms:Nore1, Rapl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1926375 Rassf5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi220C → A: Reduced interaction with HRAS. 1 Publication1
Mutagenesisi220C → D: Strongly reduced interaction with HRAS. 1 Publication1
Mutagenesisi221L → A or D: Strongly reduced interaction with HRAS. 1 Publication1
Mutagenesisi234F → A: Reduced interaction with HRAS. 1 Publication1
Mutagenesisi236K → A: Reduced interaction with HRAS. 1 Publication1
Mutagenesisi280D → A: Reduced interaction with HRAS. 1 Publication1
Mutagenesisi283K → A: Very strong reduction of the interaction with HRAS. 1 Publication1
Mutagenesisi284Q → A: Reduced interaction with HRAS. 1 Publication1
Mutagenesisi302K → D: Reduced specificity for HRAS and diminished discrimination between HRAS and RAP1A. 1 Publication1
Mutagenesisi303K → A: Strongly reduced interaction with HRAS. 1 Publication1

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002404021 – 413Ras association domain-containing protein 5Add BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177PhosphoserineCombined sources1
Modified residuei274PhosphoserineBy similarity1
Modified residuei347PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5EBH1
PRIDEiQ5EBH1

PTM databases

iPTMnetiQ5EBH1
PhosphoSitePlusiQ5EBH1
SwissPalmiQ5EBH1

Expressioni

Gene expression databases

BgeeiENSMUSG00000026430 Expressed in 258 organ(s), highest expression level in thymus
ExpressionAtlasiQ5EBH1 baseline and differential
GenevisibleiQ5EBH1 MM

Interactioni

Subunit structurei

Interacts directly with activated HRAS; a RASSF5-STK4/MST1 complex probably associates with activated HRAS. Interacts with KRAS. Probably interacts with Ras-like GTPases RRAS, RRAS2, MRAS, RAP1B, RAP2A and RALA. Can self-associate. Interacts with RSSF1 isoform A. The RSSF1 isoform A-RSSF5 heterodimer probably mediates the association of RSSF1 with HRAS. Isoform 2 interacts with activated RAP1A and ITGAL/LFA-1. Binds STK4/MST1, inhibiting STK4/MST1 autoactivation (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207620, 1 interactor
DIPiDIP-29107N
IntActiQ5EBH1, 19 interactors
MINTiQ5EBH1
STRINGi10090.ENSMUSP00000027688

Structurei

Secondary structure

1413
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ5EBH1
SMRiQ5EBH1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5EBH1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini265 – 359Ras-associatingPROSITE-ProRule annotationAdd BLAST95
Domaini361 – 408SARAHPROSITE-ProRule annotationAdd BLAST48

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri117 – 165Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST49

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IPAE Eukaryota
ENOG4111HS2 LUCA
GeneTreeiENSGT00390000003367
HOGENOMiHOG000013025
HOVERGENiHBG054362
InParanoidiQ5EBH1
KOiK08015
OrthoDBiEOG091G0EPB
PhylomeDBiQ5EBH1
TreeFamiTF319243

Family and domain databases

CDDicd00029 C1, 1 hit
InterProiView protein in InterPro
IPR033614 C-RASSF
IPR002219 PE/DAG-bd
IPR000159 RA_dom
IPR033623 RASSF5
IPR011524 SARAH_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR22738 PTHR22738, 1 hit
PTHR22738:SF9 PTHR22738:SF9, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF16517 Nore1-SARAH, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00314 RA, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50200 RA, 1 hit
PS50951 SARAH, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q5EBH1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASPAIGQRP YPLLLDPEPP RYLQSLGGTE PPPPARPRRC IPTALIPAAG
60 70 80 90 100
ASEDRGGRRS GRRDPEPTPR DCRHARPVRP GLQPRLRLRP GSHRPRDVRS
110 120 130 140 150
IFEQPQDPRV LAERGEGHRF VELALRGGPG WCDLCGREVL RQALRCANCK
160 170 180 190 200
FTCHSECRSL IQLDCRQKGG PALDRRSPES TLTPTLNQNV CKAVEETQHP
210 220 230 240 250
PTIQEIKQKI DSYNSREKHC LGMKLSEDGT YTGFIKVHLK LRRPVTVPAG
260 270 280 290 300
IRPQSIYDAI KEVNPAATTD KRTSFYLPLD AIKQLHISST TTVSEVIQGL
310 320 330 340 350
LKKFMVVDNP QKFALFKRIH KDGQVLFQKL SIADYPLYLR LLAGPDTDVL
360 370 380 390 400
SFVLKENETG EVEWDAFSIP ELQNFLTILE KEEQDKIHQL QKKYNKFRQK
410
LEEALRESQG KPG
Length:413
Mass (Da):46,714
Last modified:March 15, 2005 - v1
Checksum:iE6FF7DDE6BECB180
GO
Isoform 2 (identifier: Q5EBH1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-142: Missing.
     143-188: ALRCANCKFT...ESTLTPTLNQ → MTVDSSMSSG...RNLQSKQPSK

Show »
Length:265
Mass (Da):30,339
Checksum:iCF0431ED3B2D23D6
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z6G2D3Z6G2_MOUSE
Ras association domain-containing p...
Rassf5
321Annotation score:
A0A1D5RM65A0A1D5RM65_MOUSE
Ras association domain-containing p...
Rassf5
313Annotation score:

Sequence cautioni

The sequence BAD21397 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179E → G in AAC08580 (PubMed:9488663).Curated1
Sequence conflicti251 – 257IRPQSIY → SGPSPSM in AAC08580 (PubMed:9488663).Curated7

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0193681 – 142Missing in isoform 2. 3 PublicationsAdd BLAST142
Alternative sequenceiVSP_019369143 – 188ALRCA…PTLNQ → MTVDSSMSSGYCSLDEELED CFFTAKTTFFRNLQSKQPSK in isoform 2. 3 PublicationsAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053959 mRNA Translation: AAC08580.1
AY261333 mRNA Translation: AAP83361.1
AK131147 mRNA Translation: BAD21397.1 Different initiation.
AK088751 mRNA Translation: BAC40546.1
AK155534 mRNA Translation: BAE33312.1
AK155869 mRNA Translation: BAE33472.1
BC089605 mRNA Translation: AAH89605.1
CCDSiCCDS15268.1 [Q5EBH1-1]
CCDS78675.1 [Q5EBH1-2]
RefSeqiNP_001298023.1, NM_001311094.2 [Q5EBH1-2]
NP_061220.2, NM_018750.4 [Q5EBH1-1]
UniGeneiMm.248291

Genome annotation databases

EnsembliENSMUST00000027688; ENSMUSP00000027688; ENSMUSG00000026430 [Q5EBH1-1]
ENSMUST00000068564; ENSMUSP00000067011; ENSMUSG00000026430 [Q5EBH1-2]
GeneIDi54354
KEGGimmu:54354
UCSCiuc007cnc.1 mouse [Q5EBH1-2]
uc007cnd.1 mouse [Q5EBH1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRASF5_MOUSE
AccessioniPrimary (citable) accession number: Q5EBH1
Secondary accession number(s): O70407, Q6KAR0, Q8C2E8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 15, 2005
Last modified: September 12, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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