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UniProtKB - Q5EB81 (NB5R1_RAT)
Protein
NADH-cytochrome b5 reductase 1
Gene
Cyb5r1
Organism
Rattus norvegicus (Rat)
Status
Functioni
NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
By similarityCatalytic activityi
- EC:1.6.2.2
Cofactori
FADBy similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 136 – 166 | FADBy similarityAdd BLAST | 31 | |
Nucleotide bindingi | 175 – 210 | FADBy similarityAdd BLAST | 36 |
GO - Molecular functioni
- cytochrome-b5 reductase activity, acting on NAD(P)H Source: UniProtKB-EC
- FAD binding Source: GO_Central
GO - Biological processi
- sterol biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | FAD, Flavoprotein, NAD |
Enzyme and pathway databases
Reactomei | R-RNO-114608, Platelet degranulation R-RNO-1237044, Erythrocytes take up carbon dioxide and release oxygen |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Cyb5r1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1306084, Cyb5r1 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 8 – 28 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000287547 | 1 – 305 | NADH-cytochrome b5 reductase 1Add BLAST | 305 |
Proteomic databases
jPOSTi | Q5EB81 |
PaxDbi | Q5EB81 |
PTM databases
iPTMneti | Q5EB81 |
PhosphoSitePlusi | Q5EB81 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 156 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 113 |
Sequence similaritiesi
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0534, Eukaryota |
InParanoidi | Q5EB81 |
OrthoDBi | 1311668at2759 |
PhylomeDBi | Q5EB81 |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR19370, PTHR19370, 1 hit |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q5EB81-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGIQPSPVLL ASLGVGLFTL FGLALGTYLV RRSRRPQVTL QDPDEKYLLR
60 70 80 90 100
LLDKTTVSHN TRRFRFALPT AHHILGLPVG KHVYLSARID GSQVIRPYTP
110 120 130 140 150
VTSDEDQGYV DLVIKVYLKG VHPKFSEGGK MSQYLDSLKI GDVVEFRGPS
160 170 180 190 200
GLLSYAGKGN FNIQPNKKSP PELRVAKKLG MIAGGTGITP MLQLIRAILK
210 220 230 240 250
VPEDPTQCFL LFANQTEKDI ILREDLEELQ AQYPIRFKLW FTLDYPPEDW
260 270 280 290 300
TYSKGFVTAD MIQEHLPAPA EDVLLLLCGP PPMVQLACHP NLDKLGYSQK
MRFTY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V9S0 | G3V9S0_RAT | NADH-cytochrome b5 reductase | Cyb5r1 rCG_46248 | 305 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC089945 mRNA Translation: AAH89945.1 |
RefSeqi | NP_001013144.1, NM_001013126.1 |
Genome annotation databases
GeneIDi | 304805 |
KEGGi | rno:304805 |
UCSCi | RGD:1306084, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC089945 mRNA Translation: AAH89945.1 |
RefSeqi | NP_001013144.1, NM_001013126.1 |
3D structure databases
SMRi | Q5EB81 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000057400 |
PTM databases
iPTMneti | Q5EB81 |
PhosphoSitePlusi | Q5EB81 |
Proteomic databases
jPOSTi | Q5EB81 |
PaxDbi | Q5EB81 |
Genome annotation databases
GeneIDi | 304805 |
KEGGi | rno:304805 |
UCSCi | RGD:1306084, rat |
Organism-specific databases
CTDi | 51706 |
RGDi | 1306084, Cyb5r1 |
Phylogenomic databases
eggNOGi | KOG0534, Eukaryota |
InParanoidi | Q5EB81 |
OrthoDBi | 1311668at2759 |
PhylomeDBi | Q5EB81 |
Enzyme and pathway databases
Reactomei | R-RNO-114608, Platelet degranulation R-RNO-1237044, Erythrocytes take up carbon dioxide and release oxygen |
Miscellaneous databases
PROi | PR:Q5EB81 |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR19370, PTHR19370, 1 hit |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NB5R1_RAT | |
Accessioni | Q5EB81Primary (citable) accession number: Q5EB81 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | March 15, 2005 | |
Last modified: | February 23, 2022 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families