UniProtKB - Q5E971 (TMEDA_BOVIN)
Transmembrane emp24 domain-containing protein 10
TMED10
Functioni
Cargo receptor involved in protein vesicular trafficking and quality control in the endoplasmic reticulum (ER) and Golgi. The p24 protein family is a group of transmembrane proteins that bind coat protein complex I/COPI and coat protein complex II/COPII involved in vesicular trafficking between the membranes. Acts at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and involved in vesicle coat formation at the cytoplasmic side. Mainly functions in the early secretory pathway and cycles between the ER, ER-Golgi intermediate compartment (ERGIC) and Golgi, mediating cargo transport through COPI and COPII-coated vesicles. In COPII vesicle-mediated anterograde transport, involved in the transport of GPI-anchored proteins by acting together with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER (By similarity).
Recognizes GPI anchors structural remodeled in the ER by the GPI inositol-deacylase/PGAP1 and the metallophosphoesterase MPPE1/PGAP5 (By similarity).
In COPI vesicle-mediated retrograde transport, involved in the biogenesis of COPI vesicles and vesicle coat recruitment. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent from the modulation of gamma-secretase activity) (By similarity).
Involved in the KDELR2-mediated retrograde transport of the toxin A subunit (CTX-A-K63)together with COPI and the COOH terminus of KDELR2 (By similarity).
On Golgi membranes, acts as primary receptor for ARF1-GDP, a GTP-binding protein involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2 which mediates the hydrolysis of ARF1-bound GTP and therefore modulates protein trafficking from the Golgi apparatus. Involved in the exocytic trafficking of G protein-coupled receptors F2LR1/PAR2 (trypsin and tryspin-like enzyme receptor), OPRM1 (opioid receptor) and P2RY4 (UTD and UDP receptor) from the Golgi to the plasma membrane, thus contributing to receptor resensitization. In addition to its cargo receptor activity, may also act as a protein channel after oligomerization, facilitating the post-translational entry of leaderless cytoplasmic cargo into the ERGIC. Involved in the translocation into ERGIC, the vesicle entry and the secretion of leaderless cargos (lacking the secretion signal sequence), including the mature form of interleukin 1/IL-1 family members, the alpha-crystallin B chain HSPB5, the carbohydrate-binding proteins galectin-1/LGALS1 and galectin-3/LGALS3, the microtubule-associated protein Tau/MAPT, and the annexin A1/ANXA1; the translocation process is dependent on cargo protein unfolding and enhanced by chaperones HSP90AB1 and HSP90B1/GRP9. Could also associates with the presenilin-dependent gamma-secretase complex in order to regulate gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40/Abeta40 (By similarity).
By similarityMiscellaneous
GO - Molecular functioni
- protein transmembrane transporter activity Source: UniProtKB
- syntaxin binding Source: Ensembl
GO - Biological processi
- COPI-coated vesicle budding Source: UniProtKB
- cytosol to ERGIC protein transport Source: UniProtKB
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
- Golgi organization Source: GO_Central
- intracellular protein transport Source: GO_Central
- negative regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process Source: Ensembl
- positive regulation of interleukin-1 production Source: UniProtKB
- positive regulation of protein secretion Source: UniProtKB
- protein localization to ERGIC Source: UniProtKB
- regulated exocytosis Source: Ensembl
- regulation of amyloid-beta formation Source: UniProtKB
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: UniProtKB
Keywordsi
Biological process | ER-Golgi transport, Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Transmembrane emp24 domain-containing protein 10Short name: Protein TMED10 Alternative name(s): 21 kDa transmembrane-trafficking protein Transmembrane protein Tmp21 p24 family protein delta-1 Short name: p24delta1 |
Gene namesi | Name:TMED10 Synonyms:TMP21 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000005694 |
VGNCi | VGNC:35935, TMED10 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type I membrane protein Sequence analysis
- cis-Golgi network membrane By similarity; Single-pass type I membrane protein Sequence analysis
- trans-Golgi network membrane By similarity; Single-pass type I membrane protein Sequence analysis
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein Sequence analysis
Other locations
- Endoplasmic reticulum-Golgi intermediate compartment membrane By similarity; Single-pass type I membrane protein Sequence analysis
- secretory vesicle membrane By similarity; Single-pass type I membrane protein Sequence analysis
- Melanosome By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- gamma-secretase complex Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- COPI-coated vesicle Source: UniProtKB
- COPII-coated ER to Golgi transport vesicle Source: GO_Central
- endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
- endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
- melanosome Source: UniProtKB-SubCell
- secretory granule membrane Source: UniProtKB
- trans-Golgi network transport vesicle Source: UniProtKB
- transport vesicle membrane Source: UniProtKB-SubCell
- zymogen granule membrane Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 32 – 185 | LumenalCuratedAdd BLAST | 154 | |
Transmembranei | 186 – 206 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 207 – 219 | CytoplasmicCuratedAdd BLAST | 13 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | By similarityAdd BLAST | 31 | |
ChainiPRO_0000010398 | 32 – 219 | Transmembrane emp24 domain-containing protein 10Add BLAST | 188 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 171 | Dimethylated arginineBy similarity | 1 | |
Modified residuei | 176 | Dimethylated arginineBy similarity | 1 | |
Glycosylationi | 179 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, MethylationProteomic databases
PaxDbi | Q5E971 |
PeptideAtlasi | Q5E971 |
PRIDEi | Q5E971 |
Interactioni
Subunit structurei
Predominantly dimeric and to a lesser extent monomeric in the ER. Monomer and dimer in ERGIC and cis-Golgi network. Forms homooligomer (via GOLD domain); the assembly is promoted by direct binding with leaderless cargos and may form a protein channel that facilitates cargo entry into the ERGIC. Forms heterooligomeric complexes with other members of the p24 family such as TMED2, TMED7 and TMED9.
Interacts (via GOLD domain) with TMED2 (via GOLD domain); the complex is required for export of TMED10 from the ER to the cis-Golgi network; the complex is proposed to be involved in cis-Golgi network dynamics and / or biogenesis. Associates with the COPI vesicle coat subunits (coatomer) (By similarity). Tetramerization of the cytoplasmic domain at the Golgi membrane in vitro; the complex is proposed to interact with COPI coatomer and induce budding of the vesicles (By similarity).
Interacts with COPG1; the interaction involves TMED10 homodimer.
Interacts with ARF1 (GDP-bound); the interaction probably involves a TMED10 oligomer.
Interacts with SEC23A, SEC24B, SEC24C and SEC24D components of the coat protein complex II/COPII, indicative of an association of TMED10 with the COPII vesicle coat.
Interacts with CD59.
Interacts with MPPE1/PGAP5; the complex might recruit and sort GPI-anchored proteins to the ER-exit site, or the interaction might lead to recycling of PGAP5 between the ER and the Golgi.
Interacts with F2LR1/PAR2 (By similarity).
Interacts with KDELR2/ERD2; the interaction is disrupted by KDELR2 ligand (By similarity).
Found in a complex composed at least of SURF4, TMED2 and TMED10. Associates with the presenilin-dependent gamma-secretase complex.
Interacts with STX17; the interaction is direct.
Interacts with IL-1; the interaction is direct.
Interacts with RAB21 (active GTP-bound form); the interaction is indirect and regulates TMED10 abundance and localization at the Golgi (By similarity).
By similarityGO - Molecular functioni
- syntaxin binding Source: Ensembl
Protein-protein interaction databases
IntActi | Q5E971, 1 interactor |
MINTi | Q5E971 |
STRINGi | 9913.ENSBTAP00000007481 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 193 | GOLDPROSITE-ProRule annotationAdd BLAST | 153 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 142 | Required for interaction with STX17By similarityAdd BLAST | 142 | |
Regioni | 147 – 178 | Required for TMED10 and TMED2 cis-Golgi network localizationBy similarityAdd BLAST | 32 | |
Regioni | 204 – 219 | Interaction with COPG1By similarityAdd BLAST | 16 | |
Regioni | 207 – 219 | Interaction with ARF1 and IL1BBy similarityAdd BLAST | 13 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 211 – 219 | COPI vesicle coat-bindingSequence analysis | 9 | |
Motifi | 211 – 212 | COPII vesicle coat-bindingSequence analysis | 2 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1691, Eukaryota |
GeneTreei | ENSGT00550000074954 |
InParanoidi | Q5E971 |
OMAi | AMGNDYH |
OrthoDBi | 1370889at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR015720, Emp24-like IPR009038, GOLD_dom |
PANTHERi | PTHR22811, PTHR22811, 1 hit |
Pfami | View protein in Pfam PF01105, EMP24_GP25L, 1 hit |
SMARTi | View protein in SMART SM01190, EMP24_GP25L, 1 hit |
PROSITEi | View protein in PROSITE PS50866, GOLD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSGSSGPQAQ RGPCPFALLL LLLLGPSSVL AISFHLPVNS RKCLREEIHK
60 70 80 90 100
DLLVTGAYEI TDQSGGAGGL RTHLKITDSA GHILYSKEDA TKGKFAFTTE
110 120 130 140 150
DYDMFEVCFE SKGTGRIPDQ LVILDMKHGV EAKNYEEIAK VEKLKPLEVE
160 170 180 190 200
LRRLEDLSES IVNDFAYMKK REEEMRDTNE STNTRVLYFS IFSMFCLIGL
210
ATWQVFYLRR FFKAKKLIE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT021049 mRNA Translation: AAX09066.1 BC123529 mRNA Translation: AAI23530.1 |
RefSeqi | NP_001035639.1, NM_001040549.2 |
Genome annotation databases
Ensembli | ENSBTAT00000007481; ENSBTAP00000007481; ENSBTAG00000005694 |
GeneIDi | 529761 |
KEGGi | bta:529761 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT021049 mRNA Translation: AAX09066.1 BC123529 mRNA Translation: AAI23530.1 |
RefSeqi | NP_001035639.1, NM_001040549.2 |
3D structure databases
AlphaFoldDBi | Q5E971 |
SMRi | Q5E971 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q5E971, 1 interactor |
MINTi | Q5E971 |
STRINGi | 9913.ENSBTAP00000007481 |
Proteomic databases
PaxDbi | Q5E971 |
PeptideAtlasi | Q5E971 |
PRIDEi | Q5E971 |
Genome annotation databases
Ensembli | ENSBTAT00000007481; ENSBTAP00000007481; ENSBTAG00000005694 |
GeneIDi | 529761 |
KEGGi | bta:529761 |
Organism-specific databases
CTDi | 10972 |
VEuPathDBi | HostDB:ENSBTAG00000005694 |
VGNCi | VGNC:35935, TMED10 |
Phylogenomic databases
eggNOGi | KOG1691, Eukaryota |
GeneTreei | ENSGT00550000074954 |
InParanoidi | Q5E971 |
OMAi | AMGNDYH |
OrthoDBi | 1370889at2759 |
Gene expression databases
ExpressionAtlasi | Q5E971, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR015720, Emp24-like IPR009038, GOLD_dom |
PANTHERi | PTHR22811, PTHR22811, 1 hit |
Pfami | View protein in Pfam PF01105, EMP24_GP25L, 1 hit |
SMARTi | View protein in SMART SM01190, EMP24_GP25L, 1 hit |
PROSITEi | View protein in PROSITE PS50866, GOLD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TMEDA_BOVIN | |
Accessioni | Q5E971Primary (citable) accession number: Q5E971 Secondary accession number(s): A4FV04 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | March 15, 2005 | |
Last modified: | May 25, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families